Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Marybeth Maloney"'
Autor:
Liang Tian, Peter M. Conway, Nicholas D. Cervenka, Jingxuan Cui, Marybeth Maloney, Daniel G. Olson, Lee R. Lynd
Publikováno v:
Biotechnology for Biofuels, Vol 12, Iss 1, Pp 1-13 (2019)
Abstract Background Biofuel production from plant cell walls offers the potential for sustainable and economically attractive alternatives to petroleum-based products. In particular, Clostridium thermocellum is a promising host for consolidated biopr
Externí odkaz:
https://doaj.org/article/55d97db947c44bc683c80351f73f11cb
Autor:
Ayşenur Eminoğlu, Sean Jean-Loup Murphy, Marybeth Maloney, Anthony Lanahan, Richard J. Giannone, Robert L. Hettich, Shital A. Tripathi, Ali Osman Beldüz, Lee R. Lynd, Daniel G. Olson
Publikováno v:
Biotechnology for Biofuels, Vol 10, Iss 1, Pp 1-11 (2017)
Abstract Background With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20–31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenting organisms to
Externí odkaz:
https://doaj.org/article/217b7814e549411fa3dba26bbe1bcdd0
Autor:
Daniel G. Olson, Marybeth Maloney, Anthony A. Lanahan, Shuen Hon, Loren J. Hauser, Lee R. Lynd
Publikováno v:
Metabolic Engineering Communications, Vol 2, Iss , Pp 23-29 (2015)
A key tool for metabolic engineering is the ability to express heterologous genes. One obstacle to gene expression in non-model organisms, and especially in relatively uncharacterized bacteria, is the lack of well-characterized promoters. Here we tes
Externí odkaz:
https://doaj.org/article/4e77930198a348569cc263c7a75ba496
Autor:
Nicholas D. Cervenka, Peter M. Conway, Daniel G. Olson, Lee R. Lynd, Jingxuan Cui, Marybeth Maloney, Liang Tian
Publikováno v:
Biotechnology for Biofuels, Vol 12, Iss 1, Pp 1-13 (2019)
Biotechnology for Biofuels
Biotechnology for Biofuels
Background Biofuel production from plant cell walls offers the potential for sustainable and economically attractive alternatives to petroleum-based products. In particular, Clostridium thermocellum is a promising host for consolidated bioprocessing
Autor:
Marybeth Maloney, Daniel G. Olson, Aron W. Fenton, Qingling Tang, Lee R. Lynd, Jeffrey L. Bose, Christopher A. Fenton
Publikováno v:
Frontiers in Microbiology
Frontiers in Microbiology, Vol 12 (2021)
Frontiers in Microbiology, Vol 12 (2021)
The pyruvate kinase (PYK) isozyme from Thermoanaerobacterium saccharolyticum (TsPYK) has previously been used in metabolic engineering for improved ethanol production. This isozyme belongs to a subclass of PYK isozymes that include an extra C-domain.
This protocol describes a 96-well-plate-based, enzymatic assay for reliably estimating ethanol concentrations in experimental samples in one hour. In the presence of excess NAD+, alcohol dehydrogenase (ADH) is employed to convert ethanol to acetaldeh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::51307160c9fdc76a3fe53e44837ffd45
https://doi.org/10.17504/protocols.io.brvcm62w
https://doi.org/10.17504/protocols.io.brvcm62w
Autor:
David M. Stevenson, Sean Jean-Loup Murphy, Marybeth Maloney, Daniel G. Olson, Anthony A. Lanahan, Natalie M. Ruppertsberger, Lee R. Lynd, Evert K. Holwerda, Daniel Amador-Noguez
Publikováno v:
Biotechnology for Biofuels
Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-20 (2020)
Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-20 (2020)
Background Engineering efforts targeted at increasing ethanol by modifying the central fermentative metabolism of Clostridium thermocellum have been variably successful. Here, we aim to understand this variation by a multifaceted approach including g
Publikováno v:
Metabolic Engineering Communications, Vol 2, Iss, Pp 23-29 (2015)
A key tool for metabolic engineering is the ability to express heterologous genes. One obstacle to gene expression in non-model organisms, and especially in relatively uncharacterized bacteria, is the lack of well-characterized promoters. Here we tes
Publikováno v:
Metabolic Engineering Communications, Vol 10, Iss, Pp e00122-(2020)
Thermoanaerobacterium saccharolyticum is an anaerobic thermophile that can ferment hemicellulose to produce biofuels, such as ethanol. It has been engineered to produce ethanol at high yield and titer. T. saccharolyticum uses the Embden-Meyerhof-Parn
Autor:
Daniel G. Olson, Paul P. Lin, Marybeth Maloney, Liang Tian, Shuen Hon, Lee R. Lynd, Evert K. Holwerda, Robert S. Worthen, Jingxuan Cui
Publikováno v:
Biotechnology for Biofuels
Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-11 (2018)
Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-11 (2018)
Background Clostridium thermocellum has been the subject of multiple metabolic engineering strategies to improve its ability to ferment cellulose to ethanol, with varying degrees of success. For ethanol production in C. thermocellum, the conversion o