Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Mary Shank-Retzlaff"'
Autor:
Shruti Amle, Shari Radford, Zhuoran Wang, Laura Bronsart, Pravansu Mohanty, Sankar Renu, Mary Shank-Retzlaff
Publikováno v:
Journal of Immunological Methods. 516:113460
Publikováno v:
The AAPS Journal. 24
RNA is a fundamental tool for molecular and cellular biology research. The recent COVID-19 pandemic has proved it is also invaluable in vaccine development. However, the need for cold storage to maintain RNA integrity and the practical and economic b
Autor:
Mary Shank-Retzlaff, Yolanda Peris Taverner, Pallavi Joshi, Sankar Renu, Arun Chitikela, Animesh Koneru, Zhuoran Wang, Laura Bronsart, Pravansu Mohanty
Publikováno v:
Journal of pharmaceutical sciences. 111(8)
Capillary-mediated vitrification (CMV) is a novel method for stabilizing biological molecules and complexes. CMV leverages capillary evaporation to enable rapid desiccation of aqueous solutions while avoiding both freezing and boiling. In the CMV pro
Autor:
Thorsten Verch, Mary Shank-Retzlaff, Richard R. Rustandi, Malorie Fink, Matthew C. Troutman, Carl Hofmann, Erin Marie Cannon, John W. Loughney, Cindy Pauley, Nisarg Patel
Publikováno v:
The AAPS journal. 22(6)
Monoclonal antibodies (mAbs) are widely used as critical reagents in analytical assays. While regulatory guidelines exist for stability monitoring of biopharmaceutical antibodies, they do not apply directly to the stability of mAbs used as assay reag
Publikováno v:
Bioanalysis. 10:163-180
Compared with biologics, vaccine potency assays represent a special challenge due to their unique compositions, multivalency, long life cycles and global distribution. Historically, vaccines were released using in vivo potency assays requiring immuni
Publikováno v:
Vaccine. 35:5495-5502
Vaccine in vitro potency assays are vital regulatory tests that are used to confirm the presence and concentration of an antigen of interest in a form that directly or indirectly relates to protective activity in patients. Current assays come in many
Publikováno v:
Analytical Chemistry. 89:3554-3561
Measuring vaccine potency is critical for vaccine release and is often accomplished using antibody-based ELISAs. Antibodies can be associated with significant drawbacks that are often overlooked including lot-to-lot variability, problems with cell-li
Autor:
Christopher Roselle, Dana Whitehouse, Mary Shank-Retzlaff, Sha-Ke Wang, Thorsten Verch, Silikhone Bouaraphan, Liming Guan, Thy Follmer, Fran Ansbro
Publikováno v:
Journal of Immunological Methods. 442:20-28
Dilutions are a common source of analytical error, both in terms of accuracy and precision, and a common source of analyst mistakes. When serial dilutions are used, errors compound, even when employing laboratory automation. Direct point dilutions in
Publikováno v:
Bioanalysis. 8:1451-1464
Background: Dilution bias is a major cause of immunoassay variability due to the lack of an internal standard to determine the true versus the expected dilution value. Methodology: We used an internal control to measure dilution bias in an ELISA. Acr
Publikováno v:
Analytical and Bioanalytical Chemistry. 408:3969-3979
Microtiter plate-based assays are a common tool in biochemical and analytical labs. Despite widespread use, results generated in microtiter plate-based assays are often impacted by positional bias, in which variability in raw signal measurements are