Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Mary C. Betlach"'
Autor:
Rainer Ziermann, Mary C. Betlach
Publikováno v:
BioTechniques, Vol 26, Iss 1, Pp 106-110 (1999)
Efficient polyketide synthesis derived from plasmid-borne heterologous Streptomyces polyketide synthase (PKS) gene clusters necessitates a suitable host strain. Wellcharacterized laboratory strains such as Streptomyces coelicolor or Streptomyces livi
Externí odkaz:
https://doaj.org/article/f2174c0c927a4a7a890c2a456ea2ff52
Publikováno v:
Biochemistry. 37:14937-14942
The post-polyketide synthase (PKS) biosynthetic tailoring of macrolide antibiotics usually involves one or more oxidation reactions catalyzed by cytochrome P450 monooxygenases. As the specificities of members from this class of enzymes vary significa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3985f5df366acf218bd04841de6660d
https://doi.org/10.1021/bi981699c
https://doi.org/10.1021/bi981699c
Publikováno v:
Molecular Microbiology. 16:357-364
Summary The bacterio-opsin gene (bop) of Halobacterium halobium is located within a cluster with three other genes. Growth conditions of high light intensity and low oxygen tension induce bop gene cluster expression. To identify putative regulatory f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21636b58c92b1e508fda6e83d67fa273
https://doi.org/10.1111/j.1365-2958.1995.tb02307.x
https://doi.org/10.1111/j.1365-2958.1995.tb02307.x
Autor:
Felix Gropp, Mary C. Betlach
Publikováno v:
Proceedings of the National Academy of Sciences. 91:5475-5479
Oxygen and light affect the expression of the bacterioopsin gene (bop), which encodes a light-driven proton pump in the purple membrane of Halobacterium halobium. This response is thought to be mediated by a set of genes located adjacent to the bop g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da4b21b8d3205b7b4e8a1af8b61e2b57
https://doi.org/10.1073/pnas.91.12.5475
https://doi.org/10.1073/pnas.91.12.5475
Autor:
Richard F. Shand, Alok K. Mitra, Mary C. Betlach, Robert M. Stroud, George J. Turner, Larry J. W. Miercke
Publikováno v:
Scopus-Elsevier
Highly ordered two-dimensional (2-D) crystals of Escherichia coli-expressed bacteriorhodopsin analog (e-bR) and its D96N variant (e-D96N) reconstituted in Halobacterium halobium lipids have been obtained by starting with the opsin protein purified in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c2091b1d08f690c901c565375dca445
Publikováno v:
Systematic and Applied Microbiology. 16:716-724
Summary We have detected an additional gene in the bop gene cluster of the halophilic archaeon, Halobacterium halobium . This gene, denoted blp ( bacterio - o psin l inked p roduct) is located directly beyond the 3′ terminus of the bat gene and is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ae2d44421885cb72d944fbbad5980bb7
https://doi.org/10.1016/s0723-2020(11)80345-0
https://doi.org/10.1016/s0723-2020(11)80345-0
Autor:
Felix Gropp, Mary C. Betlach
Publikováno v:
Systematic and Applied Microbiology. 16:712-715
Summary The protein, bacterio-opsin, complexed with retinal functions as a light-driven proton pump in the purple membrane of the halophilic archaeon, Halobacterium halobium . The gene encoding bacterio-opsin ( bop ) is located within a cluster of ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::46e85f4e78e5a8507550800e8df26a79
https://doi.org/10.1016/s0723-2020(11)80344-9
https://doi.org/10.1016/s0723-2020(11)80344-9
Autor:
Mary C. Betlach, Steven J. Milder, Thorgeir E. Thorgeirsson, Larry J. W. Miercke, Robert M. Stroud, Richard F. Shand, David S. Kliger
Publikováno v:
Biochemistry. 30:9133-9142
Bacteriorhodopsin (BR) with the single-site substitutions Arg-82----Gln (R82Q), Asp-85----Asn (D85N), and Asp-96----Asn (D96N) is studied with time-resolved absorption spectroscopy in the time regime from nanoseconds to seconds. Time-resolved spectra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3bc14c977fe12ebc1badb3385cb322a5
https://doi.org/10.1021/bi00102a003
https://doi.org/10.1021/bi00102a003
Autor:
Mary C. Betlach, R F Shand
Publikováno v:
Journal of Bacteriology. 173:4692-4699
The bop gene cluster consists of at least three genes: bop (bacterio-opsin), brp (bacterio-opsin-related protein), and bat (bacterio-opsin activator). We have quantitated transcript levels from these genes in a wild-type and bacterioruberin-deficient
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22370482c6b5fd6a0dfcaf51a24ae1fc
https://doi.org/10.1128/jb.173.15.4692-4699.1991
https://doi.org/10.1128/jb.173.15.4692-4699.1991
Autor:
Mary C. Betlach, Robert M. Stroud, Larry J. W. Miercke, Susan K. Fong, Richard F. Shand, Alok K. Mitra
Publikováno v:
Biochemistry. 30:3082-3088
The integral membrane protein bacterioopsin, found in the extremely halophilic archaebacterium Halobacterium halobium, was expressed in Escherichia coli as a fusion protein containing 13 heterologous amino acids at the amino terminus. The expressed p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe22bd21ff1263314f8a0f209811c893
https://doi.org/10.1021/bi00226a015
https://doi.org/10.1021/bi00226a015