Conservation of the unusual dimeric JmjC fold of JMJD7 from Drosophila melanogaster to humans

Autor: Rasheduzzaman Chowdhury, Martine I. Abboud, James Wiley, Anthony Tumber, Suzana Markolovic, Christopher J. Schofield

Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022)

Abstract The JmjC family of 2-oxoglutarate dependent oxygenases catalyse a range of hydroxylation and demethylation reactions in humans and other animals. Jumonji domain-containing 7 (JMJD7) is a JmjC (3S)-lysyl-hydroxylase that catalyses the modific

Externí odkaz: https://doaj.org/article/77195d9f25814b63ac7af57680c41a8a

Use of cyclic peptides to induce crystallization: case study with prolyl hydroxylase domain 2

Autor: Rasheduzzaman Chowdhury, Martine I. Abboud, Tom E. McAllister, Biswadip Banerji, Bhaskar Bhushan, John L. Sorensen, Akane Kawamura, Christopher J. Schofield

Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)

Abstract Crystallization is the bottleneck in macromolecular crystallography; even when a protein crystallises, crystal packing often influences ligand-binding and protein–protein interaction interfaces, which are the key points of interest for fun

Externí odkaz: https://doaj.org/article/7ffb0667b88f4bb4a12b5e6a229217a5

Lack of activity of recombinant HIF prolyl hydroxylases (PHDs) on reported non-HIF substrates

Autor: Matthew E Cockman, Kerstin Lippl, Ya-Min Tian, Hamish B Pegg, William D Figg Jnr, Martine I Abboud, Raphael Heilig, Roman Fischer, Johanna Myllyharju, Christopher J Schofield, Peter J Ratcliffe

Publikováno v: eLife, Vol 8 (2019)

Human and other animal cells deploy three closely related dioxygenases (PHD 1, 2 and 3) to signal oxygen levels by catalysing oxygen regulated prolyl hydroxylation of the transcription factor HIF. The discovery of the HIF prolyl-hydroxylase (PHD) enz

Externí odkaz: https://doaj.org/article/70d88ea4f82c4443ab41453328102032

Exploiting Electrode Nanoconfinement to Investigate the Catalytic Properties of Isocitrate Dehydrogenase (IDH1) and a Cancer-Associated Variant

Autor: Martine I. Abboud, Ryan A. Herold, Raphael Reinbold, Clare F. Megarity, Christopher J. Schofield, Fraser A. Armstrong

Publikováno v: The Journal of Physical Chemistry Letters
Herold, R A, Reinbold, R, Megarity, C F, Abboud, M I, Schofield, C J & Armstrong, F A 2021, ' Exploiting Electrode Nanoconfinement to Investigate the Catalytic Properties of Isocitrate Dehydrogenase (IDH1) and a Cancer-Associated Variant ', Journal of Physical Chemistry Letters, vol. 12, pp. 6095-6101 . https://doi.org/10.1021/acs.jpclett.1c01517

Human isocitrate dehydrogenase (IDH1) and its cancer-associated variant (IDH1 R132H) are rendered electroactive through coconfinement with a rapid NADP(H) recycling enzyme (ferredoxin-NADP+ reductase) in nanopores formed within an indium tin oxide el

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a33cc3289dfc656e1f183b5fa39b0b1e
https://doi.org/10.1021/acs.jpclett.1c01517

Biochemical and biophysical analyses of hypoxia sensing prolyl hydroxylases from Dictyostelium discoideum and Toxoplasma gondii

Autor: James Wickens, Adam P. Hardy, Anthony Tumber, Martine I. Abboud, Michael A. McDonough, Christopher T. Lohans, Rasheduzzaman Chowdhury, Christopher M. West, Kerstin Lippl, Christopher J. Schofield, Elisabete Pires, Tongri Liu

Publikováno v: The Journal of Biological Chemistry

In animals, the response to chronic hypoxia is mediated by prolyl hydroxylases (PHDs) that regulate the levels of hypoxia-inducible transcription factor α (HIFα). PHD homologues exist in other types of eukaryotes and prokaryotes where they act on n

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58832b590e90128d122e6f594a718649
https://doi.org/10.1074/jbc.ra120.013998