Controlling T cells spreading, mechanics and activation by micropatterning

Autor: Anaïs Sadoun, Martine Biarnes-Pelicot, Laura Ghesquiere-Dierickx, Ambroise Wu, Olivier Théodoly, Laurent Limozin, Yannick Hamon, Pierre-Henri Puech

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)

Abstract We designed a strategy, based on a careful examination of the activation capabilities of proteins and antibodies used as substrates for adhering T cells, coupled to protein microstamping to control at the same time the position, shape, sprea

Externí odkaz: https://doaj.org/article/ff1e48ef684a4b078137f5fce273a6b2

Naïve T lymphocytes chemotax to CCL21 but not to S1P-rich serum

Autor: Nicolas Garcia-Seyda, Solene Song, Luc David-Broglio, Christoph Matti, Marc Artinger, Martine Biarnes-Pelicot, Marie-Pierre Valignat, Daniel F. Legler, Marc Bajénoff, Olivier Theodoly

SummaryNaïve T lymphocytes traffic through the organism in their search for antigen, alternating between blood and secondary lymphoid organs. Lymphocyte homing to lymph nodes relies on the chemokine CCL21, while exit into efferent lymphatics relies

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b4d39fd115863797fd54e2b525cc4813
https://doi.org/10.1101/2023.02.03.526993

Leukocyte transmigration and longitudinal forward-thrusting force in a microfluidic Transwell device

Autor: Sophie Brustlein, Valentine Seveau de Noray, Laurene Aoun, Martine Biarnes-Pelicot, Marie-Pierre Valignat, Paulin Nègre, Cristina Gonsales, Olivier Theodoly

Publikováno v: Biophys J

Transmigration of leukocytes across blood vessels walls is a critical step of the immune response. Transwell assays examine transmigration properties in vitro by counting cells passages through a membrane; however, the difficulty of in situ imaging h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9dc0595acaea7b9c974d2078a5a9457
https://doi.org/10.1016/j.bpj.2021.03.037

Functional Mapping of Adhesiveness on Live Cells Reveals How Guidance Phenotypes Can Emerge From Complex Spatiotemporal Integrin Regulation

Autor: Sophie Brustlein, Olivier Theodoly, Martine Biarnes-Pelicot, Bernard Malissen, Philippe Robert, Dominique Touchard, Marie-Pierre Valignat, Philippe Nicolas, Nicolas Garcia-Seyda, Petra Hatoum

Publikováno v: Frontiers in Bioengineering and Biotechnology
Frontiers in Bioengineering and Biotechnology, Frontiers, 2021, 9, ⟨10.3389/fbioe.2021.625366⟩
Frontiers in Bioengineering and Biotechnology, Vol 9 (2021)
Frontiers in Bioengineering and Biotechnology, 2021, 9, ⟨10.3389/fbioe.2021.625366⟩

Immune cells have the ubiquitous capability to migrate disregarding the adhesion properties of the environment, which requires a versatile adaptation of their adhesiveness mediated by integrins, a family of specialized adhesion proteins. Each subtype

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40592890bca9e94031b9a682a53c6250
https://pubmed.ncbi.nlm.nih.gov/33898401

Amoeboid Swimming Is Propelled by Molecular Paddling in Lymphocytes

Autor: Xuan Luo, Nicolas Garcia-Seyda, Martine Biarnes-Pelicot, Mohd Suhail Rizvi, Alphée Michelot, Claire Hivroz, Jean-Baptiste Sibarita, Marie-Pierre Valignat, Chaouqi Misbah, Laurene Aoun, Alexander Farutin, Olivier Theodoly, Paulin Nègre, Sham Tlili, Rémi Galland, Solene Song, Salima Rafaï

Publikováno v: Biophysical Journal
Biophysical Journal, Biophysical Society, 2020, 119 (6), pp.1157-1177. ⟨10.1016/j.bpj.2020.07.033⟩
Biophysical Journal, 2020, 119 (6), pp.1157-1177. ⟨10.1016/j.bpj.2020.07.033⟩
Biophys J

Mammalian cells developed two main migration modes. The slow mesenchymatous mode, like crawling of fibroblasts, relies on maturation of adhesion complexes and actin fiber traction, whereas the fast amoeboid mode, observed exclusively for leukocytes a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bc84228145c1e412c1d899d51720774
https://hal.archives-ouvertes.fr/hal-03006653