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Publikováno v:
CELLULAR AND MOLECULAR LIFE SCIENCES
r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
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r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
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Spinal muscular atrophy (SMA) is a genetic disease resulting in the loss of alpha-motoneurons followed by muscle atrophy. It is caused by knock-out mutations in the survival of motor neuron 1 (SMN1) gene, which has an unaffected, but due to preferent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=RECOLECTA___::af72f90ef2e05946649e9243ebbaa4d7
https://www.fundanet.incliva.es/publicaciones/ProdCientif/PublicacionFrw.aspx?id=16836
https://www.fundanet.incliva.es/publicaciones/ProdCientif/PublicacionFrw.aspx?id=16836
Autor:
de Lamotte, Juliette Duchesne, Polentes, J��r��me, Roussange, Florine, Lesueur, L��a, Feurgard, Pauline, Perrier, Anselme, Nicoleau, Camille, Martinat, C��cile
Additional file 6: Figure S6. hMNs sensitivity to mBoNT. a EC50 curve for hMNs treated with serial doses of mBoNT for 24 h, compared to toxin-free control dose (untreated) using an antibody recognizing cleaved and uncleaved form of SNAP25 protein. DI
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49a3266c808a00f745684b318ba14367
Autor:
de Lamotte, Juliette Duchesne, Polentes, J��r��me, Roussange, Florine, Lesueur, L��a, Feurgard, Pauline, Perrier, Anselme, Nicoleau, Camille, Martinat, C��cile
Additional file 3: Figure S3. Muscle-nerve coculture protocol. Immortalized human myoblasts were seeded in specific myogenic induction medium to induce their proliferation. Day1-post-plating myoblasts, hMNs progenitors DIV 10 previously obtained with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::857afdb02face90f50ca90c7d66e98c7
Autor:
de Lamotte, Juliette Duchesne, Polentes, J��r��me, Roussange, Florine, Lesueur, L��a, Feurgard, Pauline, Perrier, Anselme, Nicoleau, Camille, Martinat, C��cile
Additional file 7: Figure S7. BoNTs effects on another line of motor neurons (56c2) connected to muscle cells and optogenetically controlled. Quantification of Ca2+ oscillations after rBoNT/A (5 nM or 0.0016 nM), mBoNT (5 nM or 0.0016 nM) or no BoNT
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f0854bb39331da300b5bcd586dc8ead
Autor:
de Lamotte, Juliette Duchesne, Polentes, J��r��me, Roussange, Florine, Lesueur, L��a, Feurgard, Pauline, Perrier, Anselme, Nicoleau, Camille, Martinat, C��cile
Additional file 5: Figure S5. Setup for optogenetic stimulation. a Myoblasts were transduced at DIV 1 with AAV2-CMV-GCaMP6f and hMNs were transduced at DIV 8 with AAV2-hSyn-ReaChR. The muscle-nerve coculture grew for two weeks and then to perform opt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::678608f056fdb401fdabaefe31e205b4
Autor:
de Lamotte, Juliette Duchesne, Polentes, J��r��me, Roussange, Florine, Lesueur, L��a, Feurgard, Pauline, Perrier, Anselme, Nicoleau, Camille, Martinat, C��cile
Additional file 1: Figure S1. hiPSCs WTSli020-B characterization and quality control. a Schematic representation of the culture and the amplification (manual and automated) of hiPSCs WTSli020-B. b Immunofluorescence analysis of common pluripotency ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc640f6ea106fcd728b9d2cf3781ae17
Autor:
de Lamotte, Juliette Duchesne, Polentes, J��r��me, Roussange, Florine, Lesueur, L��a, Feurgard, Pauline, Perrier, Anselme, Nicoleau, Camille, Martinat, C��cile
Additional file 4: Figure S4. hMNs sensitivity to rBoNT/A in a single dose. a Representative Western blot showing the cleavage of SNAP25 protein from hMNs in coculture with myotubes treated with four doses of rBoNT/A (5 nM, 1 nM, 0.001 nM, 0.00001 nM
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fc36ff52a50cae557494482ee5e730d
Autor:
de Lamotte, Juliette Duchesne, Polentes, J��r��me, Roussange, Florine, Lesueur, L��a, Feurgard, Pauline, Perrier, Anselme, Nicoleau, Camille, Martinat, C��cile
Additional file 2: Figure S2. hMNs progenitors��� differentiation. a Schematic representation of the differentiation protocol based on Maury et al. (2015) to generate hMNs progenitors DIV 10. b Gene expression analysis in hMNs DIV 14 of relevan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12991f8d01082651468edcf8afa61c15
Autor:
Lamy, C., Marret, G., Diallo, H., Berger, D., Kadi, A., Plissonnier, A-S., Vacher, S., El Beaino, Z., Klijanienko, J., Martinat, C., Jeannot, E., Martin, J., Badois, N., Lesnik, M., Dubray Vautrin, A., Choussy, O., Taouachi, R., Bieche, I., Kamal, M., Le Tourneau, C.
Publikováno v:
In Annals of Oncology October 2023 34 Supplement 2:S563-S563
