Zobrazeno 1 - 10
of 41
pro vyhledávání: '"Martin Siemann-Herzberg"'
Publikováno v:
Microbial Cell Factories, Vol 18, Iss 1, Pp 1-10 (2019)
Abstract Background In vivo protein formation is a crucial part of cellular life. The process needs to adapt to growth conditions and is exploited for the production of technical and pharmaceutical proteins in microbes such as Escherichia coli. Accor
Externí odkaz:
https://doaj.org/article/d550e71c2af24e3aa942ca67a549fa8e
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
In vitro systems are ideal setups to investigate the basic principles of biochemical reactions and subsequently the bricks of life. Cell-free protein synthesis (CFPS) systems mimic the transcription and translation processes of whole cells in a contr
Externí odkaz:
https://doaj.org/article/a8ea7fdc641e4de8ae39270103fa2546
Publikováno v:
Frontiers in Microbiology, Vol 9 (2018)
Vibrio natriegens constitutes one of the fastest-growing nonpathogenic bacteria and a potential novel workhorse for many biotechnological applications. Here, we report the development of a Vibrio-based cell-free protein synthesis system (CFPS). Speci
Externí odkaz:
https://doaj.org/article/1210ef623a1b4354b9d8f7dcd594cc76
Autor:
Jurek Failmezger, Robert Nitschel, Andrés Sánchez-Kopper, Michael Kraml, Martin Siemann-Herzberg
Publikováno v:
PLoS ONE, Vol 11, Iss 12, p e0168764 (2016)
Cell-free protein synthesis, which mimics the biological protein production system, allows rapid expression of proteins without the need to maintain a viable cell. Nevertheless, cell-free protein expression relies on active in vivo translation machin
Externí odkaz:
https://doaj.org/article/2a811dd4f8de4fa0a63ef196d92ae57e
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2433
Cell-free extracts have been researched and continuously streamlined for around 50 years. It is believed that these extracts work best when routinely obtained from exponentially growing cells to capture the most active translation system. Here we rep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::640209b8d84c81ffdcb7dacd9ab415ea
https://pubmed.ncbi.nlm.nih.gov/34985737
https://pubmed.ncbi.nlm.nih.gov/34985737
Publikováno v:
Cell-Free Gene Expression ISBN: 9781071619971
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aff0efa18fe7d31cf0385e4f6bca4f11
https://doi.org/10.1007/978-1-0716-1998-8_3
https://doi.org/10.1007/978-1-0716-1998-8_3
Autor:
Oliver Simon, Martin Siemann-Herzberg, Sabine Frana, Tobias Vallon, Björn Mückschel, Alexander Broicher, Bernhard Hauer, Ralf Takors, Beate Rendgen-Heugle, Jens Pfannenstiel, Michael Breuer, Achim Huber
Publikováno v:
Engineering in Life Sciences. 15:760-771
To smoothen the process of n-butanol formation in Pseudomonas putida KT2440, detailed knowledge of the impact of this organic solvent on cell physiology and regulation is of outmost importance. Here, we conducted a detailed systems biology study to e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6e02941ffb2aaf144e359724f0c17c44
https://doi.org/10.1002/elsc.201400051
https://doi.org/10.1002/elsc.201400051
Autor:
Rudolf Hausmann, Marius Henkel, Anke Neumann, Christoph Syldatk, Michaela Zwick, Florian Oswald, Janina Beuker, Judit Willenbacher, Sandra Baumann, Martin Siemann-Herzberg
Publikováno v:
Biochemistry and Molecular Biology Education
Bioprocess engineering is a highly interdisciplinary field of study which is strongly benefited by practical courses where students can actively experience the interconnection between biology, engineering, and physical sciences. This work describes a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95e6966c03b1405048f2167a05ab25cb
https://doi.org/10.1002/bmb.20860
https://doi.org/10.1002/bmb.20860
Publikováno v:
Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Cell-free protein synthesis is a versatile protein production system. Performance of the protein synthesis depends on highly active cytoplasmic extracts. Extracts from E. coli are believed to work best; they are routinely obtained from exponential gr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::1481e4bfc9121bc4773ce74bffd511ec
http://europepmc.org/articles/PMC5705671
http://europepmc.org/articles/PMC5705671
Publikováno v:
ACS synthetic biology. 6(10)
Cell-free (in vitro) protein synthesis (CFPS) systems provide a versatile tool that can be used to investigate different aspects of the transcription-translation machinery by reducing cells to the basic functions of protein formation. Recent improvem
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fac9844d7f60e4d226e40b2ef886d81
https://pubmed.ncbi.nlm.nih.gov/28627886
https://pubmed.ncbi.nlm.nih.gov/28627886