In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors.

Autor: Ramiro Olivera, Lucia Natalia Moro, Roberto Jordan, Carlos Luzzani, Santiago Miriuka, Martin Radrizzani, F Xavier Donadeu, Gabriel Vichera

Publikováno v: PLoS ONE, Vol 11, Iss 10, p e0164049 (2016)

The demand for equine cloning as a tool to preserve high genetic value is growing worldwide; however, nuclear transfer efficiency is still very low. To address this issue, we first evaluated the effects of time from cell fusion to activation (

Externí odkaz: https://doaj.org/article/73fd33fc34194ba39b2cd8623985070b

Disposable Micropipette Tip for Purifying DNA Fragments

Autor: Claudia Gimenez, Leandro J. Martínez, Martin Radrizzani, Mariano Grasselli, Mirna L. Sánchez

Publikováno v: Biotechnology and Bioprocess Engineering. 25:215-223

A novel functional micropipette tip for DNA purification, called DNAtip, is described based on a novel coating process. The functional layer is prepared by an internal coating with silica particles supported into a polyvinyl alcohol (PVA) film. The c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fcbb6eb9144853d3e0487e2717afe13b
https://doi.org/10.1007/s12257-019-0141-3

One Step Histological Detection and Staining of the PTEN Tumor Suppressor Protein by a Single Strand DNA

Autor: Mariano Grasselli, Martin Radrizzani, Gloria Longinotti, Susana Vighi, Claudia Perandones, Gabriel Ybarra, Juan Sebastian Yakisich, Javier Marcelo Montserrat

Publikováno v: Diagnostics
Diagnostics, Vol 11, Iss 171, p 171 (2021)
Volume 11
Issue 2

Antibodies are the most used technological tool in histochemistry. However, even with monoclonal antibodies, their standardization is difficult due to variation of biological systems as well as to variability due to the affinity and amplification of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb92985b40b985e277929e1dd25cd86b
https://pubmed.ncbi.nlm.nih.gov/33530289

In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors

Autor: Carlos Luzzani, R. Olivera, Lucía Natalia Moro, G. Vichera, Santiago Gabriel Miriuka, F. Xavier Donadeu, Martin Radrizzani, Roberto Jordan

Publikováno v: PLoS ONE
PLoS ONE, Vol 11, Iss 10, p e0164049 (2016)
Olivera, R, Moro, L N, Jordan, R, Luzzani, C, Miriuka, S, Radrizzani, M, Donadeu, F X & Vichera, G 2016, ' In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors ', PLoS ONE, vol. 11, no. 10, e0164049 . https://doi.org/10.1371/journal.pone.0164049

The demand for equine cloning as a tool to preserve high genetic value is growing worldwide; however, nuclear transfer efficiency is still very low. To address this issue, we first evaluated the effects of time from cell fusion to activation (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c0fce63dcfdc8d05b3cdb5865dc9ef7
http://europepmc.org/articles/PMC5061425

Improvement of bovine semen quality by removal of membrane-damaged sperm cells with DNA aptamers and magnetic nanoparticles

Autor: Gabriel Vichera, Juan Sebastian Yakisich, Veronica L. Farini, Gabriel Ybarra, Carla V. Camaño, Martin Radrizzani, Diego Luis Viale

Publikováno v: Journal of biotechnology. 229

In cattle, cryopreservation of semen and sex-sorting kill up to 50% of spermatozoa and decrease the success of assisted insemination (AI). Therefore, significant efforts are being carried out to improve the quality of semen prior to AI. In this work

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ce273ee5310af1fdcfe808af0bef692
https://pubmed.ncbi.nlm.nih.gov/27164256