Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Martin Poe"'
Publikováno v:
Journal of Immunological Methods. 192:37-41
The lysis of susceptible targets by efficient cytotoxic T lymphocytes (CTL) increases both with time and with the ratio of CTL to target. Simple methods for calculating a killing rate constant from the time dependence of killing and for calculating t
Autor:
H J Zweerink, D. Boulton, J T Blake, Maureen C. Gammon, Martin Poe, Joseph K. Wu, Nolan H. Sigal
Publikováno v:
Journal of Biological Chemistry. 266:98-103
Granzyme B has been purified to homogeneity from the granules of a human cytolytic lymphocyte line, Q31, in an enzymatically active form by a three-step procedure. Q31 granzyme B hydrolyzed Na-t-butyloxycarbonyl-L-alanyl-L-alanyl-L-aspartyl (Boc-Ala-
Publikováno v:
Journal of Medicinal Chemistry. 34:46-54
The inhibition constants (Kiapp) were obtained from the action of 68 2,4-diamino-5-(substituted-benzyl)pyrimidines on dihydrofolate reductase from an Escherichia coli strain MB 1428. Subsequently, these results were used to formulate appropriate quan
Publikováno v:
Archives of Biochemistry and Biophysics. 280:416-420
Electron paramagnetic resonance (EPR) spectroscopy has been used to determine the hydration numbers of Mn(II) in complexes with GDP and three forms of ras p21. EPR signals of Mn(II) in the GDP complex with viral-Harvey p21pRAS1 (Arg 12, Thr 59), p21E
Publikováno v:
Archives of Biochemistry and Biophysics. 298:757-759
The adaptation of an analytical procedure for aggrecan based upon gel-permeation chromatography to an FPLC-based protocol has significantly sped up the analysis. The faster assay has permitted determination of the kinetic constants for digestion of h
Autor:
Yvonne Luk, Paul A. Fischer, Martin Poe, J Tom Blake, Gloria C. Koo, William H. Parsons, A Talento, Joseph K. Wu, Anna Sirotina, M Nguyen
Publikováno v:
Cellular immunology. 174(2)
The precise role of the granular enzyme A (granzyme A), a serine protease, in the lytic process of cytotoxic T lymphocytes (CTL) is not clear. We have recently constructed a CTL line transfected with the antisense gene of granzyme A (a-GrA). These a-
Publikováno v:
Biochemistry. 31(21)
The number of water molecules bound to Mn2+ in the complex with a variant of Ha ras p21 and GDP has been determined by electron paramagnetic resonance (EPR) measurements in 17O-enriched water. A resolution enhancement method has been used to improve
Publikováno v:
Archives of biochemistry and biophysics. 284(1)
The synthetic antiprotease, FUT-175 (6-amidino-2-naphthyl-4-guanidinobenzoate), was found to be an extraordinarily potent and rapid inhibitor of human Q31 cytotoxic T-lymphocyte granzyme A. The granzyme A was inhibited in a time-dependent manner with
Publikováno v:
Journal of Biological Chemistry. 254:8143-8152
The effects of pH upon the C-2 resonances of the 5 histidine residues of Escherichia coli MB 1428 dihydrofolate reductase in binary complexes with methotrexate, aminopterin, folate, methopterin, and trimethoprim were studied by 300-MHz 1H nmr spectro
Publikováno v:
Journal of Biological Chemistry. 249:2710-2716
Upon binding of pyridine nucleotides to Escherichia coli MB 1428 dihydrofolate reductase, absorption bands of both enzyme and pyridine nucleotide are changed. Titration of enzyme with TPNH confirms the presence of two TPNH binding sites. Similar titr