Zobrazeno 1 - 10
of 130
pro vyhledávání: '"Martin Marek"'
Autor:
Michal Nemergut, Daniel Pluskal, Jana Horackova, Tereza Sustrova, Jan Tulis, Tomas Barta, Racha Baatallah, Glwadys Gagnot, Veronika Novakova, Marika Majerova, Karolina Sedlackova, Sérgio M. Marques, Martin Toul, Jiri Damborsky, Zbynek Prokop, David Bednar, Yves L. Janin, Martin Marek
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-20 (2023)
Abstract NanoLuc, a superior β-barrel fold luciferase, was engineered 10 years ago but the nature of its catalysis remains puzzling. Here experimental and computational techniques are combined, revealing that imidazopyrazinone luciferins bind to an
Externí odkaz:
https://doaj.org/article/02510bf81fd243d0bfed12a6fdc366d5
Autor:
Michal Nemergut, Sérgio M. Marques, Lukas Uhrik, Tereza Vanova, Marketa Nezvedova, Darshak Chandulal Gadara, Durga Jha, Jan Tulis, Veronika Novakova, Joan Planas-Iglesias, Antonin Kunka, Anthony Legrand, Hana Hribkova, Veronika Pospisilova, Jiri Sedmik, Jan Raska, Zbynek Prokop, Jiri Damborsky, Dasa Bohaciakova, Zdenek Spacil, Lenka Hernychova, David Bednar, Martin Marek
Publikováno v:
Molecular Neurodegeneration, Vol 18, Iss 1, Pp 1-25 (2023)
Abstract Background Apolipoprotein E (ApoE) ε4 genotype is the most prevalent risk factor for late-onset Alzheimer’s Disease (AD). Although ApoE4 differs from its non-pathological ApoE3 isoform only by the C112R mutation, the molecular mechanism o
Externí odkaz:
https://doaj.org/article/3de537d320b7425ab0b3c676809f76bd
Publikováno v:
Magnetochemistry, Vol 9, Iss 12, p 232 (2023)
This work aims to characterize 3D-printed structures composed of a thermoplastic material (polylactic acid (PLA)) containing a combination of magnetic particles composed of iron(III) oxide (hematite) and iron(II)–iron (III) oxide (magnetite) with v
Externí odkaz:
https://doaj.org/article/d82ee0b070d2480ca28e2fb88ebb73a9
Publikováno v:
Lubricants, Vol 11, Iss 12, p 504 (2023)
Scaled rolling contact fatigue tests, used to practically simulate the wear of the wheel and rail material under laboratory conditions, are typically classified into two categories. Tests in the first category use twin-disc stands, while the second g
Externí odkaz:
https://doaj.org/article/5467679bb965425897e02ac45ac049df
Autor:
Eloise Pavão Guerra-Slompo, Gisele Fernanda Assine Picchi-Constante, Martin Marek, Christophe Romier, Wolfgang Sippl, Nilson Ivo Tonin Zanchin
Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 102058- (2023)
Summary: Here, we describe a combined in cellulo and in vivo approach to identify compounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while ph
Externí odkaz:
https://doaj.org/article/a6ce12f930fd45ce85f6f3a2270026cc
Autor:
Dmitri Nikitin, Jan Mican, Martin Toul, David Bednar, Michaela Peskova, Patricia Kittova, Sandra Thalerova, Jan Vitecek, Jiri Damborsky, Robert Mikulik, Sarel J. Fleishman, Zbynek Prokop, Martin Marek
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 20, Iss , Pp 1366-1377 (2022)
Cardio- and cerebrovascular diseases are leading causes of death and disability, resulting in one of the highest socio-economic burdens of any disease type. The discovery of bacterial and human plasminogen activators and their use as thrombolytic dru
Externí odkaz:
https://doaj.org/article/190a12128de241f4a06bec66f667421d
Publikováno v:
Materials, Vol 16, Iss 13, p 4780 (2023)
Designing a composite, possibly strengthened by a dispersion of (fine) oxides, is a favorable way to improve the mechanical characteristics of Cu while maintaining its advantageous electric conductivity. The aim of this study was to perform mechanica
Externí odkaz:
https://doaj.org/article/8a6579130c6c455489b2ed4f52687dc1
Autor:
Andrea Schenkmayerova, Gaspar P. Pinto, Martin Toul, Martin Marek, Lenka Hernychova, Joan Planas-Iglesias, Veronika Daniel Liskova, Daniel Pluskal, Michal Vasina, Stephane Emond, Mark Dörr, Radka Chaloupkova, David Bednar, Zbynek Prokop, Florian Hollfelder, Uwe T. Bornscheuer, Jiri Damborsky
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Directed evolution commonly relies on point mutations but InDels frequently occur in evolution. Here the authors report a protein-engineering framework based on InDel mutagenesis and fragment transplantation resulting in greater catalysis and longer
Externí odkaz:
https://doaj.org/article/3c18b7a5373d42f0b49ea5eb1b1dcbad
Autor:
Gisele Fernanda Assine Picchi-Constante, Priscila Mazzocchi Hiraiwa, Martin Marek, Vanessa Zulkievicz Rogerio, Eloise Pavão Guerra-Slompo, Christophe Romier, Nilson Ivo Tonin Zanchin
Publikováno v:
STAR Protocols, Vol 3, Iss 2, Pp 101324- (2022)
Summary: This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene contain
Externí odkaz:
https://doaj.org/article/5233e0e1faa14a838c7f1926e6da97ab
Autor:
Martin Marek, Radka Chaloupkova, Tatyana Prudnikova, Yukari Sato, Pavlina Rezacova, Yuji Nagata, Ivana Kuta Smatanova, Jiri Damborsky
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 18, Iss , Pp 1352-1362 (2020)
Engineering enzyme catalytic properties is important for basic research as well as for biotechnological applications. We have previously shown that the reshaping of enzyme access tunnels via the deletion of a short surface loop element may yield a ha
Externí odkaz:
https://doaj.org/article/bfe8011d3233457283bc3c18963bc420