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Autor:
Claire E. Naylor, Jane L Holley, Martin Keyte, Nicola J. Walker, Ajit K. Basak, Julie Miller, Richard W. Titball, Graham Carter, Carr Frank J, David S. Moss, Alberto Alape-Girón, Monica Thelestam, Marietta Flores-Díaz
Publikováno v:
Archives of Biochemistry and Biophysics. 384:24-30
A panel of random mutants within the DNA encoding the carboxy-terminal domain of Clostridium perfringens alpha-toxin was constructed. Three mutants were identified which encoded alpha-toxin variants (Lys330Glu, Asp305Gly, and Asp293Ser) with reduced