Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Martin Gawlitzek"'
Autor:
Szu-Han Wang, Danming Tang, Martin Gawlitzek, Yifeng Jing, Karen Kiplinger, Dejin Zhan, Shu Pan, Patrick Ahyow, Amy Shen, Shahram Misaghi, Meg Tung, Brad Snedecor
Publikováno v:
Biotechnology Journal. 13:1700746
Consistent cell culture performance is a prerequisite to ensure product quality consistency and achieve productivity goals for the manufacture of recombinant protein therapeutics, including monoclonal antibodies. Here a peculiar observation is report
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42f99e08aaec8094019e472cf52d0664
https://doi.org/10.1002/biot.201700746
https://doi.org/10.1002/biot.201700746
Publikováno v:
Biotechnology and Bioengineering. 103:1164-1175
The effect of different cell culture conditions on N-glycosylation site-occupancy has been elucidated for two different recombinant glycoproteins expressed in Chinese hamster ovary (CHO) cells, recombinant human tissue plasminogen activator (t-PA) an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5458302c815201dd1ece1fe8e7b1b44
https://doi.org/10.1002/bit.22348
https://doi.org/10.1002/bit.22348
Autor:
Stephen Russell, Jun Luo, Martin Gawlitzek, Yun Tang, Wei-Ting Hsu, John C. Joly, Rigzen P. S. Aulakh, Inn H. Yuk, Jacob B. Mauger
Publikováno v:
Biotechnology progress. 31(1)
Recent reports highlight the impact of copper on lactate metabolism: CHO cell cultures with higher initial copper levels shift to net lactate consumption and yield lower final lactate and higher titers. These studies investigated the effects of coppe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8652c34594200e7b40b3862f552415d0
https://pubmed.ncbi.nlm.nih.gov/25311542
https://pubmed.ncbi.nlm.nih.gov/25311542
Publikováno v:
Biotechnology and Bioengineering. 68:637-646
The effect of ammonium on the glycosylation pattern of the recombinant immunoadhesin tumor necrosis factor-IgG (TNFR-IgG) produced by Chinese hamster ovary cells is elucidated in this study. TNFR-IgG is a chimeric IgG fusion protein bearing one N-lin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4adb838353de174c4b0a6074d3240bdd
https://doi.org/10.1002/(sici)1097-0290(20000620)68:6<637::aid-bit6>3.0.co
https://doi.org/10.1002/(sici)1097-0290(20000620)68:6<637::aid-bit6>3.0.co
Publikováno v:
Biotechnology and Bioengineering. 70:25-31
Human tissue-type plasminogen activator (t-PA) contains a variably occupied glycosylation site at Asn-184 in naturally produced t-PA and in t-PA produced in recombinant Chinese hamster ovary (CHO) cells. The presence of an oligosaccharide at this sit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::477b675ef3de50a0f7676c201d58fcff
https://doi.org/10.1002/1097-0290(20001005)70:1<25::aid-bit4>3.0.co
https://doi.org/10.1002/1097-0290(20001005)70:1<25::aid-bit4>3.0.co
Engineering Chinese hamster ovary cells to maximize sialic acid content of recombinant glycoproteins
Autor:
Lynne A. Krummen, David H. Peers, K. Carroll, S. Mehta, Damon I. Papac, Vanessa Chisholm, Nishit B. Modi, Martin Gawlitzek, S. Weikert, S. Tom, Steven M. Chamow, Phillip W. Berman, D. Cowfer, J. Briggs, J. Lofgren, S. Eppler
Publikováno v:
Nature Biotechnology. 17:1116-1121
We have engineered two Chinese hamster ovary cell lines secreting different recombinant glycoproteins to express high levels of human beta1,4-galactosyltransferase (GT, E.C. 2.4.1.38) and/or alpha2, 3-sialyltransferase (ST, E.C. 2.4.99.6). N-linked o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e918a6a35e9824dce64b3e05017ef032
https://doi.org/10.1038/15104
https://doi.org/10.1038/15104
Publikováno v:
Glycobiology. 9:125-131
Elevated ammonium concentrations in the medium of cultivated cells have been shown to increase the intracellular levels of uridine-5'-diphospho-N-acetylglucosamine (UDP-GlcNAc) and uridine-5'-diphospho-N-acetylgalactosamine (UDP-GalNAc; Ryll et al.,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::288d5092d1a12976ef80fa03baa13769
https://doi.org/10.1093/glycob/9.2.125
https://doi.org/10.1093/glycob/9.2.125
Publikováno v:
Biotechnology and Bioengineering. 57:518-528
The effect of different ammonium concentrations and glucosamine on baby hamster kidney (BHK)-21 cell cultures grown in continuously perfused double membrane bioreactors was investigated with respect to the final carbohydrate structures of a secretory
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99130061671530eb233d340d11a74ee9
https://doi.org/10.1002/(sici)1097-0290(19980305)57:5<518::aid-bit3>3.0.co
https://doi.org/10.1002/(sici)1097-0290(19980305)57:5<518::aid-bit3>3.0.co
Autor:
Boyan Zhang, Natarajan Vijayasankaran, Robert Kiss, Steve Meier, Yi Yang, Amy Lim, Martin Gawlitzek, Feng Li, Sharat Varma, Melissa Mun, Silvana R. Arevalo, Trevor E. Swartz
Publikováno v:
Biotechnology progress. 29(5)
As the industry moves toward subcutaneous delivery as a preferred route of drug administration, high drug substance concentrations are becoming the norm for monoclonal antibodies. At such high concentrations, the drug substance may display a more int
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7024764cbe1169772f115cc58c0a7cba
https://pubmed.ncbi.nlm.nih.gov/23804462
https://pubmed.ncbi.nlm.nih.gov/23804462
Publikováno v:
Journal of Biotechnology. 42:117-131
The N-glycosylation patterns of a genetically engineered human interleukin-2 variant glycoprotein (IL-Mu6), produced by BHK-21 cells from long-term suspension and microcarrier cultures in the presence and absence of fetal calf serum were compared. IL
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::173e444b2a5b579affbb86760fefe9f6
https://doi.org/10.1016/0168-1656(95)00065-x
https://doi.org/10.1016/0168-1656(95)00065-x