Overlapping regions of Caf20 mediate its interactions with the mRNA-5′cap-binding protein eIF4E and with ribosomes

Autor: Ebelechukwu C. Nwokoye, Eiman AlNaseem, Robert A. Crawford, Lydia M. Castelli, Martin D. Jennings, Christopher J. Kershaw, Graham D. Pavitt

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

Abstract By interacting with the mRNA 5′ cap, the translation initiation factor eIF4E plays a critical role in selecting mRNAs for protein synthesis in eukaryotic cells. Caf20 is a member of the family of proteins found across eukaryotes termed 4E-

Externí odkaz: https://doaj.org/article/a170f8d568954ea3bc06d0d1845d3a9b

GTP binding to translation factor eIF2B stimulates its guanine nucleotide exchange activity

Autor: Christopher J. Kershaw, Martin D. Jennings, Francesco Cortopassi, Margherita Guaita, Hawra Al-Ghafli, Graham D. Pavitt

Publikováno v: iScience, Vol 24, Iss 12, Pp 103454- (2021)

Summary: eIF2B is the guanine nucleotide exchange factor (GEF) required for cytoplasmic protein synthesis initiation in eukaryotes and its regulation within the integrated stress response (ISR). It activates its partner factor eIF2, thereby promoting

Externí odkaz: https://doaj.org/article/63576347261d43b39ec105640f9c4851

Interaction of the La-related protein Slf1 with colliding ribosomes maintains translation of oxidative-stress responsive mRNAs

Autor: Martin D Jennings, Priya Srivastava, Christopher J Kershaw, David Talavera, Christopher M Grant, Graham D Pavitt

Publikováno v: Nucleic Acids Research.

In response to oxidative stress cells reprogram gene expression to enhance levels of antioxidant enzymes and promote survival. In Saccharomyces cerevisiae the polysome-interacting La-related proteins (LARPs) Slf1 and Sro9 aid adaptation of protein sy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3af404b8973844e50e1fb43a1e6aaa2c
https://doi.org/10.1093/nar/gkad272

Fail-safe control of translation initiation by dissociation of eIF2α phosphorylated ternary complexes

Autor: Martin D Jennings, Christopher J Kershaw, Tomas Adomavicius, Graham D Pavitt

Publikováno v: eLife, Vol 6 (2017)

Phosphorylation of eIF2α controls translation initiation by restricting the levels of active eIF2-GTP/Met-tRNAi ternary complexes (TC). This modulates the expression of all eukaryotic mRNAs and contributes to the cellular integrated stress response.

Externí odkaz: https://doaj.org/article/75f8611e47d04d95b6a8f582950959d1

The structural basis of translational control by eIF2 phosphorylation

Autor: Yu Zhou, Zakia Latif, Margherita Guaita, Martin D. Jennings, Graham D. Pavitt, Tomas Adomavicius, Alan M. Roseman

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
Adomavicius, T, Guaita, M, Zhou, Y, Jennings, M, Latif, Z, Roseman, A & Pavitt, G D 2019, ' The Structural Basis of Translational Control by eIF2 Phosphorylation ', Nature Communications, vol. 10, no. 1, 2136 . https://doi.org/10.1038/s41467-019-10167-3
Nature Communications

Protein synthesis in eukaryotes is controlled by signals and stresses via a common pathway, called the integrated stress response (ISR). Phosphorylation of the translation initiation factor eIF2 alpha at a conserved serine residue mediates translatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22e073439f82b95c3f6992c7381d3b52
http://link.springer.com/article/10.1038/s41467-019-10167-3