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pro vyhledávání: '"Martin Šafránek"'
Autor:
Ján Krahulec, Martin Šafránek
Publikováno v:
BMC Biotechnology, Vol 21, Iss 1, Pp 1-9 (2021)
Abstract Background The aim of this study was to provide an information about the homogeneity on the level of enterokinase productivity in P. pastoris depending on different suppliers of the media components. Results In previous studies, we performed
Externí odkaz:
https://doaj.org/article/c7d135e3d4a447aba7f7fd03edefd3e2
Autor:
Martin Šafránek, Ján Krahulec
Publikováno v:
BMC Biotechnology, Vol 21, Iss 1, Pp 1-9 (2021)
BMC Biotechnology
BMC Biotechnology
Background The aim of this study was to provide an information about the homogeneity on the level of enterokinase productivity in P. pastoris depending on different suppliers of the media components. Results In previous studies, we performed the opti
Publikováno v:
Current Plant Biology, Vol 40, Iss , Pp 100385- (2024)
Adventitious roots (ARs) are post-embryonic roots that develop from non-root organs. These roots are vital for plant survival and are crucial for the clonal reproduction of valuable horticultural and forestry species. Despite their significance, ARs
Externí odkaz:
https://doaj.org/article/822e8ff621f04f6aa1e01af673a3e436
Publikováno v:
Journal of applied genetics. 61(2)
Yeast Candida utilis is considered to be a potentially advantageous expression system for production of recombinant proteins utilizable for industrial and pharmaceutical purposes. As the scientific literature is not consistent in the ploidy of this y
Autor:
Ján Krahulec, Veronika Lišková, Diana Széliová, Kristína Melicherová, Ján Turňa, Diana Hopkova, Martin Šafránek
Publikováno v:
Applied Microbiology and Biotechnology. 101:1927-1934
Enterokinase is one of the most frequently used enzymes for the removal of affinity tags from target recombinant proteins. In this study, several fermentation strategies were assayed for the production of human enterokinase in Pichia pastoris under c
Autor:
Martin Šafranek, Alain Shumbusho, Wenche Johansen, Júlia Šarkanová, Stanislav Voško, Boris Bokor, Ján Jásik, Viktor Demko
Publikováno v:
Frontiers in Plant Science, Vol 14 (2023)
Calpains are modulatory proteases that modify diverse cellular substrates and play essential roles in eukaryots. The best studied are animal cytosolic calpains. Here, we focus on enigmatic membrane-anchored calpains, their structural and functional f
Externí odkaz:
https://doaj.org/article/09ff405ffee54bcc96f6afaeec735daf
Publikováno v:
Journal of biotechnology. 236
Promoter PBAD is frequently used for heterologous gene expression due to several advantages, such as moderately high expression levels, induction by an inexpensive and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which
Publikováno v:
New Biotechnology. 31:S191