Bottom–up protein identifications from microliter quantities of individual human tear samples. Important steps towards clinical relevance

Autor: Peter Verhaert, Bharath Raghuraman Kumar, Peter P M Raus, Martijn W. H. Pinkse

Publikováno v: EuPA Open Proteomics, Vol 9, Iss, Pp 8-13 (2015)

A relatively simple combination of Schirmer strip sampling with straightforward sensitive nanoLC quadrupole-Orbitrap tandem mass spectrometry after a minimum of sample processing steps allows for replicate proteomic analysis of single human tears, i.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56dbfa552e517dfe0bc481c7ac8450a0
https://doi.org/10.1016/j.euprot.2015.06.005

The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways

Autor: Alfons J. M. Stams, Steffen Kümmel, Steffi Franke, Sjef Boeren, Antonie H. van Gelder, Diana Z. Sousa, Peter D. E. M. Verhaert, Martijn W. H. Pinkse, Carsten Vogt, Michael Visser, Mervin Pieterse

Publikováno v: Nature Communications, 9(1)
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Nature Communications, 9:239. Nature Publishing Group
Nature Communications 9 (2018) 1
Nature Communications
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP

Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14cd74d4572957ce356a949c2327539d
https://doi.org/10.1038/s41467-017-02518-9

Perchlorate and chlorate reduction by the CrenarchaeonAeropyrum pernixand two thermophilic Firmicutes

Autor: Nicolas Tsesmetzis, Bart P. Lomans, Martin G. Liebensteiner, Peter Verhaert, Alfons J. M. Stams, Martijn W. H. Pinkse, Bart Nijsse

Publikováno v: Environmental Microbiology Reports. 7:936-945

This study reports the ability of one hyperthermophilic and two thermophilic microorganisms to grow anaerobically by the reduction of chlorate and perchlorate. Physiological, genomic and proteome analyses suggest that the Crenarchaeon Aeropyrum perni

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e562aaa34f2bb149f5702b0b870be909
https://doi.org/10.1111/1758-2229.12335

Cysteine cathepsins as digestive enzymes in the spider Nephilengys cruentata

Autor: Peter D. E. M. Verhaert, Felipe J. Fuzita, Martijn W. H. Pinkse, Adriana R. Lopes

Publikováno v: Insect Biochemistry and Molecular Biology. 60:47-58

Cysteine cathepsins are widely spread on living organisms associated to protein degradation in lysosomes, but some groups of Arthropoda (Heteroptera, Coleoptera, Crustacea and Acari) present these enzymes related to digestion of the meal proteins. Al

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73337cfe415647915d8e6af3893f4d03
https://doi.org/10.1016/j.ibmb.2015.03.005

Analytical characterization of complex, biotechnological feedstocks by pH gradient ion exchange chromatography for purification process development

Autor: Martijn W. H. Pinkse, Beckley K. Nfor, Marcel Ottens, Peter Verhaert, Alexander T. Hanke, Frieder Kröner, Jürgen Hubbuch

Publikováno v: Journal of Chromatography A. 1311:55-64

The accelerating growth of the market for proteins and the growing interest in new, more complex molecules are bringing new challenges to the downstream process development of these proteins. This results in a demand for faster, more cost efficient,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a2068e4f271f683fec0a4be3d0e75a3
https://doi.org/10.1016/j.chroma.2013.08.034