The encephalomyocarditis virus Leader promotes the release of virions inside extracellular vesicles via the induction of secretory autophagy

Autor: Susanne G. van der Grein, Kyra A. Y. Defourny, Huib H. Rabouw, Soenita S. Goerdayal, Martijn J. C. van Herwijnen, Richard W. Wubbolts, Maarten Altelaar, Frank J. M. van Kuppeveld, Esther N. M. Nolte-‘t Hoen

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)

Picornaviruses can escape infected cells via packaging in extracellular vesicles (EVs). Here, van der Grein et al. show that the non-structural Leader protein of encephalomyocarditis virus (EMCV) promotes the release of EV-enclosed virus particles an

Externí odkaz: https://doaj.org/article/532460d12a0d4231b60592e26e89f23d

Human milk extracellular vesicles preserve bronchial epithelial barrier integrity and reduce TLR3‐induced inflammation in vitro

Autor: Nikita Karra, Martijn J. C. Van Herwijnen, Marca H. M. Wauben, Emily Jane Swindle, Hywel Morgan

Publikováno v: Journal of Extracellular Biology, Vol 1, Iss 9, Pp n/a-n/a (2022)

Abstract Breast milk is essential for facilitating the growth and development of infants and for providing immune protection against viral infections in the infant's airways. Yet, regulation of inflammation by milk components may be needed to reduce

Externí odkaz: https://doaj.org/article/c6ecc34500054c1ba0e7c25223d9f866

Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions

Autor: Andrea Zendrini, Lucia Paolini, Sara Busatto, Annalisa Radeghieri, Miriam Romano, Marca H. M. Wauben, Martijn J. C. van Herwijnen, Peter Nejsum, Anne Borup, Andrea Ridolfi, Costanza Montis, Paolo Bergese

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 7 (2020)

This protocol paper describes how to assign a purity grade and to subsequently titrate extracellular vesicle (EV) solutions of a few microliters in volume by microplate COlorimetric NANoplasmonic (CONAN) assay. The CONAN assay consists of a solution

Externí odkaz: https://doaj.org/article/215b279b0dd448029713e62e0d6610dd

Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures

Autor: Marijke I. Zonneveld, Alain R. Brisson, Martijn J. C. van Herwijnen, Sisareuth Tan, Chris H. A. van de Lest, Frank A. Redegeld, Johan Garssen, Marca H. M. Wauben, Esther N. M. Nolte-'t Hoen

Publikováno v: Journal of Extracellular Vesicles, Vol 3, Iss 0, Pp 1-12 (2014)

Extracellular vesicles (EV) in breast milk carry immune relevant proteins and could play an important role in the instruction of the neonatal immune system. To further analyze these EV and to elucidate their function it is important that native popul

Externí odkaz: https://doaj.org/article/026de1ff281a47fa93fa3d36eb3f8037

Large-scale production of extracellular vesicles:Report on the 'massivEVs' ISEV workshop

Autor: Lucia Paolini, Marta Monguió‐Tortajada, Marta Costa, Fabio Antenucci, Mario Barilani, Marta Clos‐Sansalvador, André Cronemberger Andrade, Tom A. P. Driedonks, Sara Giancaterino, Stephanie M. Kronstadt, Rachel R. Mizenko, Muhammad Nawaz, Xabier Osteikoetxea, Carla Pereira, Surya Shrivastava, Anders Toftegaard Boysen, Simonides Immanuel van de Wakker, Martijn J. C. van Herwijnen, Xiaoqin Wang, Dionysios C. Watson, Mario Gimona, Maria Kaparakis‐Liaskos, Konstantin Konstantinov, Sai Kiang Lim, Nicole Meisner‐Kober, Michiel Stork, Peter Nejsum, Annalisa Radeghieri, Eva Rohde, Nicolas Touzet, Marca H. M. Wauben, Kenneth W. Witwer, Antonella Bongiovanni, Paolo Bergese

Publikováno v: Paolini, L, Monguió-Tortajada, M, Costa, M, Antenucci, F, Barilani, M, Clos-Sansalvador, M, Andrade, A C, Driedonks, T A P, Giancaterino, S, Kronstadt, S M, Mizenko, R R, Nawaz, M, Osteikoetxea, X, Pereira, C, Shrivastava, S, Boysen, A T, van de Wakker, S I, van Herwijnen, M J C, Wang, X, Watson, D C, Gimona, M, Kaparakis-Liaskos, M, Konstantinov, K, Lim, S K, Meisner-Kober, N, Stork, M, Nejsum, P, Radeghieri, A, Rohde, E, Touzet, N, Wauben, M H M, Witwer, K W, Bongiovanni, A & Bergese, P 2022, ' Large-scale production of extracellular vesicles : Report on the “massivEVs” ISEV workshop ', Journal of Extracellular Biology, vol. 1, no. 10, e63 . https://doi.org/10.1002/jex2.63

Extracellular vesicles (EVs) large-scale production is a crucial point for the translation of EVs from discovery to application of EV-based products. In October 2021, the International Society for Extracellular Vesicles (ISEV), along with support by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bf556333843d4cb4aafedac1e74902e
https://pure.au.dk/portal/da/publications/largescale-production-of-extracellular-vesicles(6a8684e1-1452-43fa-b88a-e1655cc73d85).html