Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Marlies E. Oomen"'
Publikováno v:
Nature methods
Chromosome segregation requires both compaction and disentanglement of sister chromatids. We describe SisterC, a chromosome conformation capture assay that distinguishes interactions between and along identical sister chromatids. SisterC employs 5-br
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a09e40875de45a23772255274ba2e21
https://doi.org/10.1038/s41592-020-0930-9
https://doi.org/10.1038/s41592-020-0930-9
Publikováno v:
Genome Research. 29:236-249
CCCTC-binding factor (CTCF) plays a key role in the formation of topologically associating domains (TADs) and loops in interphase. During mitosis TADs are absent, but how TAD formation is dynamically controlled during the cell cycle is not known. Sev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ef42207089694445b650eb7083ed1e5
https://doi.org/10.1101/gr.241547.118
https://doi.org/10.1101/gr.241547.118
Autor:
René Maehr, Hui Mao, Job Dekker, Johan H. Gibcus, Ryan M.J. Genga, Leonid A. Mirny, Ankita Nand, Oliver J. Rando, Sergey V. Venev, Liyan Yang, Krishna Mohan Parsi, Marlies E. Oomen, Nils Krietenstein, Sameer Abraham, Betul Akgol Oksuz, Hakan Ozadam
Publikováno v:
Nature Methods
Chromosome conformation capture (3C) assays are used to map chromatin interactions genome-wide. Chromatin interaction maps provide insights into the spatial organization of chromosomes and the mechanisms by which they fold. Hi-C and Micro-C are widel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59d27ba7e69a87d6222e4a9730e95c98
https://doi.org/10.1101/2020.12.26.424448
https://doi.org/10.1101/2020.12.26.424448
Autor:
Jérôme Salignon, Alexey Chernobrovkin, Roman A. Zubarev, Benjamin A. Garcia, Marlies E. Oomen, Lluís Millan-Ariño, Christian G. Riedel, Simone Brandenburg, Lioba Körner, Zuo-Fei Yuan
Publikováno v:
Current Protocols in Protein Science, 102(1):e114
Current Protocols in Protein Science
Current Protocols in Protein Science
Histones are the major proteinaceous component of chromatin in eukaryotic cells and an important part of the epigenome, affecting most DNA‐related events, including transcription, DNA replication, and chromosome segregation. The properties of histo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ecaf74cbaf1e4c6bef93730c2cfb2aa
https://research.rug.nl/en/publications/cce5bc78-5f41-4e9e-9f5a-5ac7c0fe3ba3
https://research.rug.nl/en/publications/cce5bc78-5f41-4e9e-9f5a-5ac7c0fe3ba3
Current chromosome conformation capture techniques are not able to distinguish sister chromatids. Here we describe the protocol of SisterC1: a novel Hi-C technique that leverages BrdU incorporation and UV/Hoechst-induced single strand breaks to ident
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b33c469bd314c72dfbf50718755c1f84
https://doi.org/10.21203/rs.3.pex-1021/v1
https://doi.org/10.21203/rs.3.pex-1021/v1
Accurate chromosome segregation requires chromosome compaction with concordant disentanglement of the two sister chromatids. This process has been studied extensively by microscopy but has remained a challenge for genomic methods, such as Hi-C, becau
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d615ede7ac0f9829fb830c079f3ca468
Autor:
Victor W.T. Liu, Nicholas Rhind, Michael P. Moazami, Job Dekker, Jonathan K. Watts, Marlies E. Oomen, Adam K. Hedger
Publikováno v:
Canadian Journal of Chemistry. 96:636-645
The ability to metabolically label DNA in a way that produces a latent change from one nucleobase to another would create a signal that can be amplified by PCR — this in turn would allow studies of newly synthesized DNA using high-throughput sequen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d8482dfaaebfd2dda39fa8feda5ed67a
https://doi.org/10.1139/cjc-2017-0732
https://doi.org/10.1139/cjc-2017-0732
CTCF plays a key role in formation of topologically associating domains (TADs) and loops in interphase. During mitosis TADs are absent, but how TAD formation is dynamically controlled during the cell cycle is not known. Several contradicting observat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a09d5901beb932308dab72f9304c50d6
Autor:
Tom Misteli, Leonid A. Mirny, Gianluca Pegoraro, Hugo B. Brandão, Marlies E. Oomen, Elizabeth H. Finn, Anne-Laure Valton, Job Dekker
Publikováno v:
PMC
Several general principles of global 3D genome organization have recently been established, including non-random positioning of chromosomes and genes in the cell nucleus, distinct chromatin compartments, and topologically associating domains (TADs).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eaab159923a5e814e694455589089eb7
https://pubmed.ncbi.nlm.nih.gov/30833794
https://pubmed.ncbi.nlm.nih.gov/30833794
Autor:
Hugo B. Brandão, Gianluca Pegoraro, Elizabeth H. Finn, Job Dekker, Tom Misteli, Leonid A. Mirny, Marlies E. Oomen, Anne-Laure Valton
The genome is hierarchically organized in 3D space and its architecture is altered in differentiation, development and disease. Some of the general principles that determine global 3D genome organization have been established. However, the extent and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f2caa13d896bf462f93b6bb6c10a8ea
https://doi.org/10.1101/171801
https://doi.org/10.1101/171801