Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Markus Lubeck"'
Autor:
Denys Oliinyk, Andreas Will, Felix R Schneidmadel, Maximilian Böhme, Jenny Rinke, Andreas Hochhaus, Thomas Ernst, Nina Hahn, Christian Geis, Markus Lubeck, Oliver Raether, Sean J Humphrey, Florian Meier
Publikováno v:
Molecular Systems Biology, Vol 20, Iss 8, Pp 972-995 (2024)
Abstract Mass spectrometry has revolutionized cell signaling research by vastly simplifying the analysis of many thousands of phosphorylation sites in the human proteome. Defining the cellular response to perturbations is crucial for further illumina
Externí odkaz:
https://doaj.org/article/6e562a7488544cb3987d5a02f54ee0a2
Autor:
Andreas‐David Brunner, Marvin Thielert, Catherine Vasilopoulou, Constantin Ammar, Fabian Coscia, Andreas Mund, Ole B Hoerning, Nicolai Bache, Amalia Apalategui, Markus Lubeck, Sabrina Richter, David S Fischer, Oliver Raether, Melvin A Park, Florian Meier, Fabian J Theis, Matthias Mann
Publikováno v:
Molecular Systems Biology, Vol 18, Iss 3, Pp n/a-n/a (2022)
Abstract Single‐cell technologies are revolutionizing biology but are today mainly limited to imaging and deep sequencing. However, proteins are the main drivers of cellular function and in‐depth characterization of individual cells by mass spect
Externí odkaz:
https://doaj.org/article/ac8470a27f154fe790c1489104ae340a
Autor:
Soumya Mukherjee, Andris Jankevics, Florian Busch, Markus Lubeck, Yang Zou, Gary Kruppa, Albert J. R. Heck, Richard A. Scheltema, Karli R. Reiding
Spatial separation of ions in the gas-phase, providing information about their size as collisional cross-sections, can readily be achieved through ion mobility. The timsTOF Pro series combines a trapped ion mobility device with a quadrupole, collisio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1e81fbc05954b74870780057c9d8acd7
https://doi.org/10.1101/2022.07.04.498688
https://doi.org/10.1101/2022.07.04.498688
Autor:
Ruedi Aebersold, Ben C. Collins, Annie Ha, Stephanie Kaspar-Schoenefeld, Oliver Raether, Eugenia Voytik, Andreas-David Brunner, Max Frank, Matthias Mann, Florian Meier, Isabell Bludau, Nicolai Bache, Hannes L. Röst, Markus Lubeck
Publikováno v:
Nature Methods, 17 (12)
Meier, F, Brunner, A-D, Frank, M, Ha, A, Bludau, I, Voytik, E, Kaspar-Schoenefeld, S, Lubeck, M, Raether, O, Bache, N, Aebersold, R, Collins, B C, Roest, H L & Mann, M 2020, ' diaPASEF : parallel accumulation-serial fragmentation combined with data-independent acquisition ', Nature Methods, vol. 17, no. 12, pp. 1229-1236 . https://doi.org/10.1038/s41592-020-00998-0
Nature Methods
Meier, F, Brunner, A-D, Frank, M, Ha, A, Bludau, I, Voytik, E, Kaspar-Schoenefeld, S, Lubeck, M, Raether, O, Bache, N, Aebersold, R, Collins, B C, Röst, H L & Mann, M 2020, ' diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition ', Nature Methods, vol. 17, pp. 1229-1236 . https://doi.org/10.1038/s41592-020-00998-0
Meier, F, Brunner, A-D, Frank, M, Ha, A, Bludau, I, Voytik, E, Kaspar-Schoenefeld, S, Lubeck, M, Raether, O, Bache, N, Aebersold, R, Collins, B C, Roest, H L & Mann, M 2020, ' diaPASEF : parallel accumulation-serial fragmentation combined with data-independent acquisition ', Nature Methods, vol. 17, no. 12, pp. 1229-1236 . https://doi.org/10.1038/s41592-020-00998-0
Nature Methods
Meier, F, Brunner, A-D, Frank, M, Ha, A, Bludau, I, Voytik, E, Kaspar-Schoenefeld, S, Lubeck, M, Raether, O, Bache, N, Aebersold, R, Collins, B C, Röst, H L & Mann, M 2020, ' diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition ', Nature Methods, vol. 17, pp. 1229-1236 . https://doi.org/10.1038/s41592-020-00998-0
diaPASEF makes use of the correlation between the ion mobility and the m/z of peptides to trap and release precursor ions in a TIMS-TOF mass spectrometer for an almost complete sampling of the precursor ion beam with data-independent acquisition.Data
Autor:
Nicolai Bache, Melvin A. Park, Ole Bjeld Horning, Constantin Ammar, Florian Meier, Andreas Mund, Fabian Coscia, David S. Fischer, Marvin Thielert, Matthias Mann, Oliver Raether, Amalia Apalategui, Catherine G. Vasilopoulou, Andreas-David Brunner, Markus Lubeck, Fabian J. Theis, Sabrina Richter
Publikováno v:
bioRxiv
Mol. Syst. Biol. 18:e10798 (2022)
Brunner, A-D, Thielert, M, Vasilopoulou, C, Ammar, C, Coscia, F, Mund, A, Hoerning, O B, Bache, N, Apalategui, A, Lubeck, M, Richter, S, Fischer, D S, Raether, O, Park, M A, Meier, F, Theis, F J & Mann, M 2022, ' Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation ', Molecular Systems Biology, vol. 18, no. 3, e10798 . https://doi.org/10.15252/msb.202110798
Mol. Syst. Biol. 18:e10798 (2022)
Brunner, A-D, Thielert, M, Vasilopoulou, C, Ammar, C, Coscia, F, Mund, A, Hoerning, O B, Bache, N, Apalategui, A, Lubeck, M, Richter, S, Fischer, D S, Raether, O, Park, M A, Meier, F, Theis, F J & Mann, M 2022, ' Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation ', Molecular Systems Biology, vol. 18, no. 3, e10798 . https://doi.org/10.15252/msb.202110798
Single-cell technologies are revolutionizing biology but are today mainly limited to imaging and deep sequencing1–3. However, proteins are the main drivers of cellular function and in-depth characterization of individual cells by mass spectrometry
Publikováno v:
International Journal of Mass Spectrometry. 425:22-35
Trapped ion mobility spectrometry (TIMS) hybridized with mass spectrometry (MS) is a relatively recent advance in the field of ion mobility mass spectrometry (IMMS). The basic idea behind TIMS is the reversal of the classic drift cell analyzer. Rathe
Autor:
Hannes L. Röst, Annie Ha, Max Frank, Ruedi Aebersold, Markus Lubeck, Oliver Raether, Matthias Mann, Eugenia Voytik, Ben C. Collins, Isabell Bludau, Stephanie Kaspar-Schoenefeld, Florian Meier, Andreas-David Brunner
Bottom-up proteomics produces complex peptide populations that are identified and quantified at the precursor or fragment ion level. Data dependent acquisition methods sequentially isolate and fragment particular precursors, whereas data independent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d704f82191541c78edfd016120644b2
https://doi.org/10.1101/656207
https://doi.org/10.1101/656207
Autor:
Thomas Kosinski, Heiner Koch, Florian Meier, Melvin A. Park, Jürgen Cox, Markus Lubeck, Jens Decker, Niels Goedecke, Matthias Mann, Oliver Raether, Nicolai Bache, Michael Krause, Scarlet Koch, Andreas-David Brunner, Ole Hoerning
Publikováno v:
Molecular & Cellular Proteomics : MCP
Molecular and Cellular Proteomics
Meier, F, Brunner, A-D, Koch, S, Koch, H, Lubeck, M, Krause, M, Goedecke, N, Decker, J, Kosinski, T, Park, M A, Bache, N, Hoerning, O, Cox, J, Räther, O & Mann, M 2018, ' Online parallel accumulation-serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer ', Molecular and Cellular Proteomics, vol. 17, no. 12, pp. 2534-2545 . https://doi.org/10.1074/mcp.TIR118.000900
Molecular and Cellular Proteomics
Meier, F, Brunner, A-D, Koch, S, Koch, H, Lubeck, M, Krause, M, Goedecke, N, Decker, J, Kosinski, T, Park, M A, Bache, N, Hoerning, O, Cox, J, Räther, O & Mann, M 2018, ' Online parallel accumulation-serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer ', Molecular and Cellular Proteomics, vol. 17, no. 12, pp. 2534-2545 . https://doi.org/10.1074/mcp.TIR118.000900
PASEF multiplies the sequencing speed without any loss in sensitivity and is implemented in the timsTOF Pro instrument introduced here. Sequencing speeds above 100 Hz enable single run proteome analysis at a depth of 6000 proteins, making the instrum
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b10bebb2ea55414baa2ed538121fd70e
Autor:
Markus Lubeck, Niklas Grassl, Oliver Raether, Florian Meier, Matthias Mann, Melvin A. Park, Scarlet Beck
Publikováno v:
JOURNAL OF PROTEOME RESEARCH
In liquid chromatography-mass spectrometry (LC-MS)-based proteomics, many precursors elute from the column simultaneously. In data-dependent analyses, these precursors are fragmented one at a time, whereas the others are discarded entirely. Here we e
Autor:
Anton Pervukhin, Andreas Bertsch, Carsten Baessmann, Oliver Kohlbacher, Sebastian Böcker, Markus Lubeck, Christian G. Huber, Andreas Leinenbach, Yasser A. Elnakady, Rolf Müller, Ralf Hartmer
Publikováno v:
ELECTROPHORESIS. 30:3736-3747
De novo sequencing of peptides using tandem MS is difficult due to missing fragment ions in the spectra commonly obtained after CID of peptide precursor ions. Complementing CID spectra with spectra obtained in an ion-trap mass spectrometer upon elect