Pilus-1 Backbone Protein RrgB of Streptococcus pneumoniae Binds Collagen I in a Force-Dependent Way

Autor: Tanja D Becke, Benedikt K Kaufmann, Markus Hilleringmann, Bastian Hartmann, Arndt F. Schilling, Stefanie Sudhop, Stefan Ness, Hauke Clausen-Schaumann

Publikováno v: ACS Nano. 13:7155-7165

Attachment to host tissue is a prerequisite for successful host colonization and invasion of pathogens. Many pathogenic bacteria use surface appendices, called pili, to bind and firmly attach to host tissue surfaces. Although it has been speculated t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d2950460026b1617e8455e35ac8119d
https://doi.org/10.1021/acsnano.9b02587

Pneumococcal pili are composed of protofilaments exposing adhesive clusters of Rrg A.

Autor: Markus Hilleringmann, Fabiola Giusti, Barbara C Baudner, Vega Masignani, Antonello Covacci, Rino Rappuoli, Michèle A Barocchi, Ilaria Ferlenghi

Publikováno v: PLoS Pathogens, Vol 4, Iss 3, p e1000026 (2008)

Pili have been identified on the cell surface of Streptococcus pneumoniae, a major cause of morbidity and mortality worldwide. In contrast to Gram-negative bacteria, little is known about the structure of native pili in Gram-positive species and thei

Externí odkaz: https://doaj.org/article/afa7033223844b56ace88d4b3edfeb98

Nanometer-scale colocalization microscopy of Streptococcus pneumoniae filaments

Autor: Thomas Hellerer, Stefan Ness, Joachim O. Rädler, Markus Hilleringmann, Christoph Polzer, Mojtaba Mohseni

Publikováno v: Multiphoton Microscopy in the Biomedical Sciences XIX.

We present numerical simulations and experiments employing two-photon excited fluorescence in a sum-frequency mixing scheme which could be used for colocalization experiments in biophysics. By means of numerical calculations using the Debye approxima

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::961319a1a512a43b871030f67e387960
https://doi.org/10.1117/12.2509927

Single molecule force spectroscopy reveals two-domain binding mode of pilus-1 tip protein RrgA of $Streptococcus\ pneumoniae$ to fibronectin

Autor: Hauke Clausen-Schaumann, Raimund Gürster, Arndt F. Schilling, Tanja D Becke, Markus Hilleringmann, Stefanie Sudhop, Anne-Marie di Guilmi, Stefan Ness

Publikováno v: ACS Nano
ACS Nano, 2018, 12 (1), pp.549-558. ⟨10.1021/acsnano.7b07247⟩
ACS Nano, American Chemical Society, 2018, 12 (1), pp.549-558. ⟨10.1021/acsnano.7b07247⟩

International audience; For host cell adhesion and invasion, surface piliation procures benefits for bacteria. A detailed investigation of how pili adhere to host cells is therefore a key aspect inunderstanding their role during infection. Streptococ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ab9ce550f916d8b951192ac624ac7e9
https://hal.science/hal-01871819

The Two Variants of the Streptococcus pneumoniae Pilus 1 RrgA Adhesin Retain the Same Function and Elicit Cross-Protection In Vivo

Autor: Michèle A. Barocchi, Mariagrazia Pizza, Claudio Donati, Massimiliano Biagini, Silvia Maccari, Nathalie Norais, Werner Pansegrau, Carla Emolo, Monica Moschioni, Paolo Ruggiero, Markus Hilleringmann, Ilaria Ferlenghi, Antonia Sinisi, Vega Masignani

Publikováno v: Infection and Immunity. 78:5033-5042

Thirty percent of Streptococcus pneumoniae isolates contain pilus islet 1, coding for a pilus composed of the backbone subunit RrgB and two ancillary proteins, RrgA and RrgC. RrgA is the major determinant of in vitro adhesion associated with pilus 1,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b51f9c6e1279eb9228ddf359442b1952
https://doi.org/10.1128/iai.00601-10