Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Markus Halfar"'
Autor:
Peter J. Brixius, Ole Elvang Jensen, Markus Halfar, Inger Mollerup, Maria-Regina Kula, Jörg Thömmes
Publikováno v:
Biotechnology and Bioengineering. 93:14-20
Expanded bed adsorption (EBA) was evaluated for the isolation of the human insulin precursor MI3, expressed and secreted by the yeast Saccharomyces cerevisiae. The isoelectric point of the insulin precursor (pH 5.3) makes cation exchange a prime cand
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0866d7f05a58f032a3095463989f64e0
https://doi.org/10.1002/bit.20531
https://doi.org/10.1002/bit.20531
Autor:
Rolf Müller, Markus Halfar, Simon Esser, Michael Müller, Jörg Thömmes, Dirk Franke, Ralf Takors, Georg A. Sprenger, Christian Dose, Volker Lorbach
Publikováno v:
Chemistry - A European Journal. 9:4188-4196
Cyclohexadiene-trans-5,6-diols such as (S,S)-2,3-dihydroxy-2,3-dihydrobenzoic acid (2,3-trans-CHD) have been shown to be of importance as chiral starting materials for the syntheses of bioactive substances, especially for the syntheses of carbasugars
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4efc9511aa0525a4df49f2ab3665f3dc
https://doi.org/10.1002/chem.200204265
https://doi.org/10.1002/chem.200204265
Autor:
Dirk Weuster-Botz, S. Dilsen, D. Tippe, Markus Halfar, A. Sandgathe, Jörg Thömmes, Roland Freudl, M.-R. Kula, Jochen Meens
Publikováno v:
Process Biochemistry. 38:1351-1363
Recombinant human calcitonin (hCT) can be expressed by Staphylococcus carnosus as a precursor peptide being part of a secreted fusion protein which contains the propeptide of a Staphylococcus hyicus lipase as secretion carrier and multiple repeats of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07df98ebd72c4ab03c701797843f70b8
https://doi.org/10.1016/s0032-9592(02)00332-1
https://doi.org/10.1016/s0032-9592(02)00332-1
Publikováno v:
Process Biochemistry. 34:159-165
The interaction of a mammalian cell culture broth with two commercially available adsorbents for the use in expanded bed adsorption (EBA) has been studied. A cation exchange resin (Streamline SP) and an affinity adsorbent (Streamline rProtein A) were
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a6a3632692d58edb4eb464691da3ed13
https://doi.org/10.1016/s0032-9592(98)00083-1
https://doi.org/10.1016/s0032-9592(98)00083-1
Publikováno v:
Journal of Chromatography A. 752:111-122
A novel expanded bed adsorbent carrying a recombinant protein A ligand was used for the isolation of monoclonal antibodies from cell containing hybridoma fermentation broth. The untreated effluent from a continuous hybridoma cultivation was applied t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9d95909d39d83ffcbd1e6463f81f340
https://doi.org/10.1016/s0021-9673(96)00504-3
https://doi.org/10.1016/s0021-9673(96)00504-3
Publikováno v:
Biotechnology and Bioengineering. 45:205-211
To achive the coarse purification of a monoclonal antibody from whole hybridoma fermentation broth a fluidized bed cation exchange process was used. The procedure consisted of application of the crude sample and washing of the bed in a fluidized mode
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::177c352cfe59bdbe8e128deea5e39a0c
https://doi.org/10.1002/bit.260450304
https://doi.org/10.1002/bit.260450304
Autor:
Maria-Regina Kula, Jörg Thömmes, Holger Gieren, Simon Curvers, Ralf Takors, Markus Halfar, Rainer Brunschier
Publikováno v:
Biotechnology progress 17, 503-512 (2001). doi:10.1021/bp990296g
The purification of human chymotrypsinogen B (hCTRB) after expression and secretion by the yeast Pichia pastoris is described based on two different approaches using integrated initial recovery. Extraction employing aqueous two-phase systems (ATPS) f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adf192f6d6e8b605c6a3fc3879c1c094
https://hdl.handle.net/2128/2439
https://hdl.handle.net/2128/2439
Autor:
Dirk Franke, Volker Lorbach, Simon Esser, Christian Dose, Georg A. Sprenger, Markus Halfar, Jörg Thömmes, Rolf Müller, Ralf Takors, Michael Müller
Publikováno v:
Chemistry - A European Journal; Sep2003, Vol. 9 Issue 17, p4188-4196, 9p