Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Markus Durm"'
Autor:
Michael Hausmann, I. Sorokine-Durm, Markus Durm, Frank-Martin Haar, Christoph Cremer, Philippe Voisin, Horst Ludwig
Publikováno v:
Cytometry. 31:153-162
Fluorescence in situ hybridization (FISH) has become a powerful tool in chromosome analysis. This report describes the systematic optimization of the Fast-FISH technique for centromere labeling of human metaphase chromosomes for radiobiological dosim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3b12cd84cb6bc74ff03c718079896b2
https://doi.org/10.1002/(sici)1097-0320(19980301)31:3<153::aid-cyto2>3.0.co
https://doi.org/10.1002/(sici)1097-0320(19980301)31:3<153::aid-cyto2>3.0.co
Publikováno v:
Zeitschrift für Naturforschung C. 52:82-88
In all DNA-DNA in situ hybridization (ISH) procedures described so far in the literature, the production of single-stranded target DNA sequences plays a decisive role. This can be achieved either by enzymatic treatment at physiological temperatures o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c75466d5c7b8617f512fe4465aad71f5
https://doi.org/10.1515/znc-1997-1-215
https://doi.org/10.1515/znc-1997-1-215
Publikováno v:
Brazilian Journal of Medical and Biological Research. 30:15-22
It has been shown for several DNA probes that the recently introduced Fast-FISH (fluorescence in situ hybridization) technique is well suited for quantitative microscopy. For highly repetitive DNA probes the hybridization (renaturation) time and the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f19f1d087e9dc8c604de9785359e14e0
https://doi.org/10.1590/s0100-879x1997000100003
https://doi.org/10.1590/s0100-879x1997000100003
Publikováno v:
Zeitschrift für Naturforschung C. 51:435-440
The technique of chromosome-in-situ suppression (ClSS)-hybridization (chromosome painting) has now been well established. However, all standard protocols so far require long renaturation times (typically 12 hours and more). Here, we describe a new, e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9038936da7a3ed87207238dcdfcbed40
https://doi.org/10.1515/znc-1996-5-625
https://doi.org/10.1515/znc-1996-5-625
Publikováno v:
Zeitschrift für Naturforschung C. 51:253-262
A rapid FISH (fluorescence in situ hybridization) technique (Fast-FISH) for quantitative microscopy has been recently introduced. For highly repetitive D N A probes the hybridization (renaturation) time and the number of necessary washing steps were
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c29d77720c3ef9af5f72db3ce0c3bbdf
https://doi.org/10.1515/znc-1996-3-418
https://doi.org/10.1515/znc-1996-3-418
Autor:
Ralph Winkler, Alexander Rapp, Markus Durm, Birgit Perner, Christoph Cremer, Karl Otto Greulich, Michael Hausmann
Publikováno v:
Journal of microscopy. 209(Pt 1)
Summary A non-enzymatic, low temperature fluorescence in situ hybridization (LTFISH) procedure was applied to metaphase spreads and interphase cell nuclei. In this context ‘low temperature’ means that the denaturation procedure of the chromosomal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e4b89c818d558ff7e3fa970eed9907a
https://pubmed.ncbi.nlm.nih.gov/12535181
https://pubmed.ncbi.nlm.nih.gov/12535181
Autor:
Christoph Cremer, Horst Ludwig, Michael Hausmann, H Münch, Markus Durm, L. Schussler, Jeffrey M. Craig
Publikováno v:
BioTechniques. 24(5)
For chromosome painting, in situ suppression of repetitive DNA sequences has been well established. Such standard protocols usually require large amounts of Cot-1 DNA®. Recently, it has become possible to deplete repetitive DNA sequences from librar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef3bfe97a796aad41d6fbc5ce1c542b2
https://pubmed.ncbi.nlm.nih.gov/9591132
https://pubmed.ncbi.nlm.nih.gov/9591132
Publikováno v:
Journal of biochemical and biophysical methods. 33(1)
It has been shown for several highly repetitive DNA probes that the newly introduced Fast-FISH (fast-fluorescence in situ hybridization) technique is well suited for quantitative microscopy. The advantage of omitting denaturing chemical agents (e.g.,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2071b5267fbf8506ec44294d38d5e2fc
https://pubmed.ncbi.nlm.nih.gov/8905467
https://pubmed.ncbi.nlm.nih.gov/8905467
'In Situ' Estimates of the Spatial Resolution for 'Practical' Fluorescence Microscopy of Cell Nuclei
Autor:
Joachim Bradl, Horst Ludwig, Bernhard Schneider, Michael Hausmann, Markus Durm, Christoph Cremer, Bernd Rinke
Publikováno v:
Fluorescence Microscopy and Fluorescent Probes ISBN: 9781489918680
Axial and lateral responses obtained from ideal point objects through a fluorescence light microscope can be used to calculate the spatial resolution of the system from the point spread function. In practice, however, the experimental conditions give
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2b80eeebdc0e211a8e02228a5878413d
https://doi.org/10.1007/978-1-4899-1866-6_24
https://doi.org/10.1007/978-1-4899-1866-6_24
Autor:
Michael Hausmann, Horst Ludwig, Klaus Aldinger, Christoph Cremer, Markus Durm, Frank-Martin Haar, Dino Celeda
Publikováno v:
Cytometry. 17(1)
Fluorescence in situ hybridization (FISH) has become an important tool not only in cytogenetic research but also in routine clinical chromosome diagnostics. Here, results of a quantification of fluorescence signals after in situ hybridization with re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6535bf4fe61d8d2040b2aa65f298c162
https://pubmed.ncbi.nlm.nih.gov/8001456
https://pubmed.ncbi.nlm.nih.gov/8001456