Zobrazeno 1 - 10
of 99
pro vyhledávání: '"Markus A. Seeger"'
Autor:
Benedikt T. Kuhn, Jonathan Zöller, Iwan Zimmermann, Tim Gemeinhardt, Dogukan H. Özkul, Julian D. Langer, Markus A. Seeger, Eric R. Geertsma
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-12 (2024)
Abstract Uptake of nucleobases and ascorbate is an essential process in all living organisms mediated by SLC23 transport proteins. These transmembrane carriers operate via the elevator alternating-access mechanism, and are composed of two rigid domai
Externí odkaz:
https://doaj.org/article/ff069676171e4d63a4d2f96eb75cc12a
Autor:
J. Josephine Botsch, Roswitha Junker, Michèle Sorgenfrei, Patricia P. Ogger, Luca Stier, Susanne von Gronau, Peter J. Murray, Markus A. Seeger, Brenda A. Schulman, Bastian Bräuning
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024)
Abstract Transmembrane E3 ligases play crucial roles in homeostasis. Much protein and organelle quality control, and metabolic regulation, are determined by ER-resident MARCH6 E3 ligases, including Doa10 in yeast. Here, we present Doa10/MARCH6 struct
Externí odkaz:
https://doaj.org/article/83b8992d9946492ea98c04163ae4e8c3
Autor:
Sille Remm, Dario De Vecchis, Jendrik Schöppe, Cedric A. J. Hutter, Imre Gonda, Michael Hohl, Simon Newstead, Lars V. Schäfer, Markus A. Seeger
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-17 (2023)
Abstract Mycobacterium tuberculosis is protected from antibiotic therapy by a multi-layered hydrophobic cell envelope. Major facilitator superfamily (MFS) transporter Rv1410 and the periplasmic lipoprotein LprG are involved in transport of triacylgly
Externí odkaz:
https://doaj.org/article/1960b492e8a54571b9bda14e84f5dd05
Autor:
Mathieu Botte, Dongchun Ni, Stephan Schenck, Iwan Zimmermann, Mohamed Chami, Nicolas Bocquet, Pascal Egloff, Denis Bucher, Matilde Trabuco, Robert K. Y. Cheng, Janine D. Brunner, Markus A. Seeger, Henning Stahlberg, Michael Hennig
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Lateral opening of the LptDE transporter in the outer membrane of Gram-negative bacteria is necessary for insertion of lipopolysaccharides. Here, Botte et al. report a cryo-EM structure of a partially opened LptDE transporter, in complex with rigid c
Externí odkaz:
https://doaj.org/article/25e3d626c5e9434d8732b67049815a5b
Autor:
Markus A. Seeger
Publikováno v:
CHIMIA, Vol 76, Iss 12 (2022)
Externí odkaz:
https://doaj.org/article/6117caf1280548e6b02a2ac5d097af04
Autor:
Dawid Deneka, Sonja Rutz, Cedric A. J. Hutter, Markus A. Seeger, Marta Sawicka, Raimund Dutzler
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Volume-regulated anion channels (VRACs) are heteromers of LRRC8 proteins, all containing the obligatory subunit LRRC8A. Here, the authors develop and characterize nanobodies that bind LRRC8A and allosterically modulate the function of homomeric LRRC8
Externí odkaz:
https://doaj.org/article/4caeef50636c4f189a4fe60799bf32be
Autor:
Tingting Li, Hongmin Cai, Hebang Yao, Bingjie Zhou, Ning Zhang, Martje Fentener van Vlissingen, Thijs Kuiken, Wenyu Han, Corine H. GeurtsvanKessel, Yuhuan Gong, Yapei Zhao, Quan Shen, Wenming Qin, Xiao-Xu Tian, Chao Peng, Yanling Lai, Yanxing Wang, Cedric A. J. Hutter, Shu-Ming Kuo, Juan Bao, Caixuan Liu, Yifan Wang, Audrey S. Richard, Hervé Raoul, Jiaming Lan, Markus A. Seeger, Yao Cong, Barry Rockx, Gary Wong, Yuhai Bi, Dimitri Lavillette, Dianfan Li
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Here, the authors report the engineering, structural and biological characterization of synthetic nanobodies (sybodies) that display potent therapeutic activity against SARS-CoV-2 infection in animal models via targeting the virus receptor-binding do
Externí odkaz:
https://doaj.org/article/e2d2ef9db7c042d5ae0d4a35e2484adb
Autor:
Gianluca Trinco, Valentina Arkhipova, Alisa A. Garaeva, Cedric A. J. Hutter, Markus A. Seeger, Albert Guskov, Dirk J. Slotboom
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
Trinco et al. measure aspartate uptake rates in proteoliposomes containing purified prokaryotic Na+-coupled aspartate transporter GltTk. To overcome limitation of protein orientation, they use synthetic nanobody that blocks transporters from outside
Externí odkaz:
https://doaj.org/article/76bc5140efca4c9482b7af7a5ab080f1
Autor:
Hongmin Cai, Hebang Yao, Tingting Li, Cedric A. J. Hutter, Yanfang Li, Yannan Tang, Markus A. Seeger, Dianfan Li
Publikováno v:
Communications Biology, Vol 3, Iss 1, Pp 1-16 (2020)
In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels
Externí odkaz:
https://doaj.org/article/baab4a34e2bb410bbf23d2caba607e37
Autor:
Tânia F. Custódio, Hrishikesh Das, Daniel J. Sheward, Leo Hanke, Samuel Pazicky, Joanna Pieprzyk, Michèle Sorgenfrei, Martin A. Schroer, Andrey Yu. Gruzinov, Cy M. Jeffries, Melissa A. Graewert, Dmitri I. Svergun, Nikolay Dobrev, Kim Remans, Markus A. Seeger, Gerald M. McInerney, Ben Murrell, B. Martin Hällberg, Christian Löw
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Here, the authors isolate several nanobodies from a synthetic library that bind the receptor-binding domain (RBD) of SARS-CoV-2 spike protein (S) and neutralize S pseudotyped viruses. Cryo-EM structure of Spike with one nanobody and further biophysic
Externí odkaz:
https://doaj.org/article/b79a9adc234e460f9baa5ef6524bc6bf