Molecular features of steroid-binding antidins and their use for assaying serum progesterone.

Autor: Nitin Agrawal, Soili I Lehtonen, Meri Uusi-Mäkelä, Purvi Jain, Sari Viitala, Juha A E Määttä, Niklas Kähkönen, Latifeh Azizi, Tiina A Riihimäki, Markku S Kulomaa, Mark S Johnson, Vesa P Hytönen, Tomi T Airenne

Publikováno v: PLoS ONE, Vol 14, Iss 2, p e0212339 (2019)

Chicken avidin (Avd) and streptavidin from Streptomyces avidinii are extensively used in bionanotechnology due to their extremely tight binding to biotin (Kd ~ 10-15 M for chicken Avd). We previously reported engineered Avds known as antidins, which

Externí odkaz: https://doaj.org/article/addaf38daa174d15b4a834c6349f099d

A novel chimeric avidin with increased thermal stability using DNA shuffling.

Autor: Barbara Taskinen, Tomi T Airenne, Janne Jänis, Rolle Rahikainen, Mark S Johnson, Markku S Kulomaa, Vesa P Hytönen

Publikováno v: PLoS ONE, Vol 9, Iss 3, p e92058 (2014)

Avidins are a family of proteins widely employed in biotechnology. We have previously shown that functional chimeric mutant proteins can be created from avidin and avidin-related protein 2 using a methodology combining random mutagenesis by recombina

Externí odkaz: https://doaj.org/article/e9426aca06ff43049770e7730ba55592

Zebavidin--an avidin-like protein from zebrafish.

Autor: Barbara Taskinen, Joanna Zmurko, Markus Ojanen, Sampo Kukkurainen, Marimuthu Parthiban, Juha A E Määttä, Jenni Leppiniemi, Janne Jänis, Mataleena Parikka, Hannu Turpeinen, Mika Rämet, Marko Pesu, Mark S Johnson, Markku S Kulomaa, Tomi T Airenne, Vesa P Hytönen

Publikováno v: PLoS ONE, Vol 8, Iss 10, p e77207 (2013)

The avidin protein family members are well known for their high affinity towards D-biotin and high structural stability. These properties make avidins valuable tools for a wide range of biotechnology applications. We have identified a new member of t

Externí odkaz: https://doaj.org/article/67b93c0e76414e249b27e076ce4178f7

Bifunctional avidin with covalently modifiable ligand binding site.

Autor: Jenni Leppiniemi, Juha A E Määttä, Henrik Hammaren, Mikko Soikkeli, Mikko Laitaoja, Janne Jänis, Markku S Kulomaa, Vesa P Hytönen

Publikováno v: PLoS ONE, Vol 6, Iss 1, p e16576 (2011)

The extensive use of avidin and streptavidin in life sciences originates from the extraordinary tight biotin-binding affinity of these tetrameric proteins. Numerous studies have been performed to modify the biotin-binding affinity of (strept)avidin t

Externí odkaz: https://doaj.org/article/1371767d19524fb4a9a0ef731d4811d5

Artificial avidin-based receptors for a panel of small molecules

Autor: Markku S. Kulomaa, Sampo Kukkurainen, Tiina A. Riihimäki, Nitin Agrawal, Masi Koskinen, Mark S. Johnson, Antti Tullila, Niklas Kähkönen, Tomi T. Airenne, Tarja K. Nevanen, Soili I. Lehtonen, Vesa P. Hytönen

Publikováno v: Lehtonen, S I, Tullila, A, Agrawal, N, Kukkurainen, S, Kähkönen, N, Koskinen, M, Nevanen, T K, Johnson, M S, Airenne, T T, Kulomaa, M S, Riihimäki, T A & Hytönen, V P 2016, ' Artificial avidin-based receptors for a panel of small molecules ', ACS Chemical Biology, vol. 11, no. 1, pp. 211-221 . https://doi.org/10.1021/acschembio.5b00906

Proteins with high specificity, affinity, and stability are needed for biomolecular recognition in a plethora of applications. Antibodies are powerful affinity tools, but they may also suffer from limitations such as low stability and high production

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6a031479973723878b09d21e72ca27c
https://cris.vtt.fi/en/publications/79e78d3d-a693-4568-8141-75eb28c1b107