Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Mark Tingey"'
Publikováno v:
Frontiers in Cellular Neuroscience, Vol 18 (2024)
The cilium, a pivotal organelle crucial for cell signaling and proper cell function, relies on meticulous macromolecular transport from the cytoplasm for its formation and maintenance. While the intraflagellar transport (IFT) pathway has traditionall
Externí odkaz:
https://doaj.org/article/3123d609d8a542908da595d800807866
Publikováno v:
STAR Protocols, Vol 5, Iss 1, Pp 102790- (2024)
Summary: Here, we present a protocol for single-molecule super-resolution imaging of the nuclear export of pre-ribosomal subunits pre-40S and pre-60S through nuclear pore complexes. We describe steps for plating cells and co-transfecting cells. We th
Externí odkaz:
https://doaj.org/article/ecb031eb0df04a1da9a76ad6b3c4e97f
Publikováno v:
Chemical & Biomedical Imaging, Vol 1, Iss 4, Pp 356-371 (2023)
Super-resolution imaging techniques have broken the diffraction-limited resolution of light microscopy. However, acquiring three-dimensional (3D) super-resolution information about structures and dynamic processes in live cells at high speed remains
Externí odkaz:
https://doaj.org/article/6804c6c4544e459eae67e1045dcd1e39
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 11 (2023)
The nuclear pore complex (NPC) serves as a pivotal subcellular structure, acting as a gateway that orchestrates nucleocytoplasmic transport through a selectively permeable barrier. Nucleoporins (Nups), particularly those containing phenylalanine–gl
Externí odkaz:
https://doaj.org/article/e2d165ac76db4095986e3a98bba0a801
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 21, Iss , Pp 1424-1432 (2023)
Super-resolution imaging techniques have provided unprecedentedly detailed information by surpassing the diffraction-limited resolution of light microscopy. However, in order to derive high quality spatial resolution, many of these techniques require
Externí odkaz:
https://doaj.org/article/982b1c5198934d87b443381314f0e02c
Autor:
Samuel L. Junod, Mark Tingey, Joseph M. Kelich, Alexander Goryaynov, Karl Herbine, Weidong Yang
Publikováno v:
iScience, Vol 26, Iss 8, Pp 107445- (2023)
Summary: We present a study on the nuclear export efficiency and time of pre-ribosomal subunits in live mammalian cells, using high-speed single-molecule tracking and single-molecule fluorescence resonance energy transfer techniques. Our findings rev
Externí odkaz:
https://doaj.org/article/3b2355e5d5fb4310a9da2de9470f1453
Publikováno v:
Nucleus, Vol 13, Iss 1, Pp 170-193 (2022)
The Nuclear Pore Complex (NPC) represents a critical passage through the nuclear envelope for nuclear import and export that impacts nearly every cellular process at some level. Recent technological advances in the form of Auxin Inducible Degron (AID
Externí odkaz:
https://doaj.org/article/e6631a46618544c8b5097a23d257fa73
Autor:
Charles R. Dixon, Poonam Malik, Jose I. de las Heras, Natalia Saiz-Ros, Flavia de Lima Alves, Mark Tingey, Eleanor Gaunt, A. Christine Richardson, David A. Kelly, Martin W. Goldberg, Greg J. Towers, Weidong Yang, Juri Rappsilber, Paul Digard, Eric C. Schirmer
Publikováno v:
iScience, Vol 24, Iss 9, Pp 103055- (2021)
Summary: STimulator of INterferon Genes (STING) is an adaptor for cytoplasmic DNA sensing by cGAMP/cGAS that helps trigger innate immune responses (IIRs). Although STING is mostly localized in the ER, we find a separate inner nuclear membrane pool of
Externí odkaz:
https://doaj.org/article/7c77bb3feb48478187feaae2f2cec97e
Autor:
Mark Tingey, Steven J. Schnell, Wenlan Yu, Jason Saredy, Samuel Junod, Dhrumil Patel, Abdullah A. Alkurdi, Weidong Yang
Publikováno v:
Cells, Vol 11, Iss 19, p 3079 (2022)
The transient nature of RNA has rendered it one of the more difficult biological targets for imaging. This difficulty stems both from the physical properties of RNA as well as the temporal constraints associated therewith. These concerns are further
Externí odkaz:
https://doaj.org/article/4c5d090503524a7ba2f22229197a1587
Publikováno v:
STAR Protocols, Vol 2, Iss 2, Pp 100490- (2021)
Summary: Single-molecule fluorescence recovery after photobleaching (smFRAP) is a newly developed technique that combines single-molecule super-resolution microscopy and traditional FRAP microscopy. smFRAP enables researchers to measure the dynamics,
Externí odkaz:
https://doaj.org/article/a0365e73a53641ada0e9cee32f4ddc3b