A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in Escherichia coli

Autor: Adrian J. Jervis, Erik K.R. Hanko, Mark S. Dunstan, Christopher J. Robinson, Eriko Takano, Nigel S. Scrutton

Publikováno v: Microbial Biotechnology, Vol 14, Iss 3, Pp 1120-1129 (2021)

Summary CRISPR technologies have become standard laboratory tools for genetic manipulations across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR tools for engineering bacteria has been slower than for eukaryotes;

Externí odkaz: https://doaj.org/article/93c6321b959a442383f2e02ce5235557

Supplementary Tables 1 - 2 from In Silico Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling

Autor: Ian J. Stratford, David Leys, Katherine A. Scott, Mary C. Caraher, Mark S. Dunstan, Karen A. Nolan

PDF file - 162KB

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91637ee495634b28dc1386478c47e286
https://doi.org/10.1158/1535-7163.22497628.v1

Data from In Silico Screening Reveals Structurally Diverse, Nanomolar Inhibitors of NQO2 That Are Functionally Active in Cells and Can Modulate NF-κB Signaling

Autor: Ian J. Stratford, David Leys, Katherine A. Scott, Mary C. Caraher, Mark S. Dunstan, Karen A. Nolan

The National Cancer Institute chemical database has been screened using in silico docking to identify novel nanomolar inhibitors of NRH:quinone oxidoreductase 2 (NQO2). The inhibitors identified from the screen exhibit a diverse range of scaffolds an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0795f7e8755ff509725dbaeeab6bba29
https://doi.org/10.1158/1535-7163.c.6535672

SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution

Autor: Joanna C, Sadler, Neil, Swainston, Mark S, Dunstan, Andrew, Currin, Douglas B, Kell

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2461

Directed evolution of proteins is a highly effective strategy for tailoring biocatalysts to a particular application, and is capable of engineering improvements such as k

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4f7317bd2ee04563d65bfe0d4988268e
https://pubmed.ncbi.nlm.nih.gov/35727444

Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers

Autor: William Rowe, Kirk J. Malone, Nigel S. Scrutton, Jason Micklefield, Perdita E. Barran, Rainer Breitling, Adrian J. Jervis, Katherine A. Hollywood, Reynard Spiess, Philip Shapira, Sandra Taylor, Eriko Takano, Pablo Carbonell, Christopher J. Robinson, Andrew Currin, Nicholas J. Turner, Steven Parker, Neil Swainston, Rosalind Le Feuvre, Nicholas E. Matthews, Mark S. Dunstan, Paul Mulherin, Cunyu Yan

Publikováno v: Metabolic Engineering
Robinson, C, Carbonell, P, Jervis, A, Yan, C, Hollywood, K, Currin, A, Swainston, N, Speiss, R, Taylor, S, Mulherin, P, Parker, S, Rowe, W, Matthews, N, Malone, K, Le Feuvre, R, Shapira, P, Barran, P, Turner, N, Micklefield, J, Breitling, R, Takano, E & Scrutton, N 2020, ' Rapid prototyping of microbial production strains for the biomanufacture of potential material monomers ', Metabolic Engineering . https://doi.org/10.1016/j.ymben.2020.04.008

Bio-based production of industrial chemicals using synthetic biology can provide alternative green routes from renewable resources, allowing for cleaner production processes. To efficiently produce chemicals on-demand through microbial strain enginee

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fb2bb76545a1cee3c07d3fd5e981a13
http://europepmc.org/articles/PMC7225752