Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Mark S. Berninger"'
Publikováno v:
Analytical Biochemistry. 206:91-97
A simple and rapid method for cloning of amplification products directly from the polymerase chain reaction (PCR) has been developed. The method is based on the addition of a 12-base dUMP-containing sequence (CUACUACUACUA) to the 5' end of PCR primer
Autor:
Mark S. Berninger, Irena Bronstein, John D. Burczak, Eric Buxton, Jeanne Carr, Max A. Chernesky, Shanfun Ching, Anne Daigle, M.S. Dey, J.A. Down, Ann M. Drevon, Chris Duffy, Eoin Fahy, Francois Ferre, Soumitra S. Ghosh, Thomas R. Gingeras, Richard L. Hodinka, D.R. Howard, Hsiang-Yun Hu, S.R. Jurgensen, W.E. Keating, Helen H. Lee, M.C. Little, James B. Mahony, Lawrence T. Malek, Annie Marchese, Bruce J. McCreedy, Nelson L. Michael, J.G. Nadeau, V.R. Neece, Gerard J. Nuovo, C.M. Nycz, Corinne E.M. Olesen, Patrick Pezzoli, Ayoub Rashtchian, David M. Schuster, Roy Sooknanan, C.A. Spargo, Jay Stoerker, Daniel L. Stoler, Mickey S. Urdea, Bob van Gemen, G.T. Walker, A.H. Walters, Danny L. Wiedbrauk, Judith C. Wilbur, P. Zwadyk
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::18a3f596031a09d84e56af79267df559
https://doi.org/10.1016/b978-012748920-9/50000-5
https://doi.org/10.1016/b978-012748920-9/50000-5
Publikováno v:
Molecular Methods for Virus Detection
This chapter describes an isothermal method for amplification of nucleic acids based on ligation of a promoter sequence to a target nucleic acid, resulting in target amplification through a transcribed RNA intermediate. This target amplification meth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d16b68a079a48aa004298e244f229b18
https://doi.org/10.1016/b978-012748920-9/50017-0
https://doi.org/10.1016/b978-012748920-9/50017-0
Publikováno v:
Gene. 93(1)
Polymerase chain reactions (PCRs) synthesize abundant amplification products. Contamination of new PCRs with trace amounts of these products, called carry-over contamination, yields false positive results. Carry-over contamination from some previous
Publikováno v:
Analytical biochemistry. 135(2)
A strategy employing T4 DNA polymerase replacement synthesis is described whereby only the insert portion of recombinant plasmids are radioisotopically labeled. Prior purification of the inserted DNA is not required. The recombinant plasmid is first
Publikováno v:
Gene. 49(3)
A novel method for detecting specific DNA sequences is described. The method uses a non-radioactive DNA probe, called a probe-vector, that can transform competent Escherichia coli cells at high efficiency only when it has hybridized to a specific DNA