Identification of Mycobacterium Species from BACTEC MGIT Positive Cultures with Oligo-FISH and PNA-FISH Methods

Autor: Gürol Emekdaş, Mark J. Fiandaca, Natuschka M. Lee, Esin Aydin, Gulay Borekci, Yusuf Ozkul, Gönül Aslan, Henrik Stender

Publikováno v: Mikrobiyoloji Bulteni. 48:385-401

Rapid and accurate diagnosis of mycobacteria is very important in the prevention and effective treatment of tuberculosis which is still a serious public health problem. Fluorescence in situ hybridization (FISH) method using rRNA targeted probes allow

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::492935682e850d87926d58f48a75b7e3
https://doi.org/10.5578/mb.7876

Pseudomonas aeruginosabiofilms in the respiratory tract of cystic fibrosis patients

Autor: C.R. Hansen, Peter Østrup Jensen, Tacjana Pressler, Thomas Bjarnsholt, Niels Høiby, Mark J. Fiandaca, Michael Givskov, Claus B. Andersen, Jette Pedersen

Publikováno v: Pediatric Pulmonology. 44:547-558

The present study was undertaken to investigate the appearance and location of Pseudomonas aeruginosa in the cystic fibrosis (CF) lung and in sputum. Samples include preserved tissues of CF patients who died due to chronic P. aeruginosa lung infectio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ca727b242a0737972fc991ec2c33a490
https://doi.org/10.1002/ppul.21011

Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures

Autor: Mark J. Fiandaca, Henrik Stender, Mette Søgaard, Dennis S. Hansen, Henrik Carl Schønheyder

Publikováno v: Aalborg University
Søgaard, M, Hansen, D S, Fiandaca, M J, Stender, H & Schønheyder, H C 2007, ' Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures ', Journal of Medical Microbiology, vol. 56, no. 7, pp. 914-917 .

This study evaluated a novel peptide nucleic acid (PNA) probe targeting a region of the 23S rRNA gene of Klebsiella pneumoniae by fluorescence in situ hybridization (FISH). Analytical performance was determined using 39 reference strains and other we

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a289f0f807dfd7a8bd7417c82cb7e31
https://doi.org/10.1099/jmm.0.46829-0

PNA Beacons for Duplex DNA

Autor: James C. Coull, Heiko Kuhn, Mark J. Fiandaca, Brian D. Gildea, Maxim D. Frank-Kamenetskii, Vadim V. Demidov

Publikováno v: Antisense and Nucleic Acid Drug Development. 11:265-270

We report here on the hybridization of peptide nucleic acid (PNA)-based molecular beacons (MB) directly to duplex DNA sites locally exposed by PNA openers. Two stemless PNA beacons were tested, both featuring the same recognition sequence and fluorop

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74b47a2248ac49bec5b809946e0e7e24
https://doi.org/10.1089/108729001317022269

Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH method: a multicenter investigation

Autor: Will Harris, Georgia Colasante, Kevin C. Hazen, Rebecca J. Buckner, Sharon Stevens, Thomas E. Davis, Michelle C. Meltzer, Mark J. Fiandaca, Martin Fuchs, Fann Wu, Benjamin Crystal, Daniel Lindao, D. Fuller, Margie Morgan, Phyllis Della Latta, Erica S. Anderson, James M. Coull, Janeen Shepard, Lisa L. Klimas, Melissa K. Deck

Publikováno v: Diagnostic microbiology and infectious disease. 78(4)

The performance of a diagnostic method for detection and identification of Enterococcus spp. directly from positive blood culture was evaluated in a clinical study. The method, Enterococcus QuickFISH BC, is a second-generation peptide nucleic acid (P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfa0e1e1967f9449cee81b0268048afa
https://pubmed.ncbi.nlm.nih.gov/24439447