Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Mark H, Harpster"'
Publikováno v:
Analyst
A microscale biosensing platform using rehydration-mediated swelling of bio-functionalized hydrogel structures and rapid target analyte capture is described. Induced convective flow mitigates diffusion limited incubation times, enabling model assays
Autor:
Teresa E. Lehmann, Mark H. Harpster, Kevin E. Macartney, Shelby E. Follett, Teresa M. Reilly, Azure D. Ingersoll, Sally A. Murray
Publikováno v:
JBIC Journal of Biological Inorganic Chemistry. 22:121-136
The antibiotics known as bleomycins constitute a family of natural products clinically employed for the treatment of a wide spectrum of cancers. The drug acts as an antitumor agent by virtue of the ability of a metal complex of the antibiotic to clea
Publikováno v:
Langmuir. 28:4030-4037
A magnetic capture-based, surface-enhanced Raman scattering (SERS) assay for DNA detection has been developed which utilizes Au-coated paramagnetic nanoparticles (Au@PMPs) as both a SERS substrate and effective bioseparation reagent for the selective
Publikováno v:
Analytical Chemistry. 83:254-260
A model paramagnetic nanoparticle (MNP) assay is demonstrated for surface-enhanced Raman scattering (SERS) detection of DNA oligonucleotides derived from the West Nile virus (WNV) genome. Detection is based on the capture of WNV target sequences by h
Autor:
James O. Mecham, William C. Wilson, Patrick A. Johnson, Hao Zhang, Jing Neng, Mark H. Harpster
Publikováno v:
Biosensors and Bioelectronics. 26:1009-1015
A surface enhanced Raman scattering (SERS) immunoassay for antibody detection in serum is described in the present work. The developed assay is conducted in solution and utilizes Au nanoparticles coated with the envelope (E) protein of West Nile Viru
Autor:
Joseph M. Palys, Mark H. Harpster, John M. Labavitch, David A. Brummell, David S. Reid, Mary S. Kalamaki
Publikováno v:
Journal of Agricultural and Food Chemistry. 51:7456-7464
Processing tomato lines suppressed in the accumulation of ripening-related polygalacturonase or expansin were generated by introduction of transgenes to silence expression of the LePG and LeExp1 genes, respectively. The rheological properties of juic
Autor:
Joseph M. Palys, Mark H. Harpster, Neal Gutterson, David A. Brummell, Peter J. Balint-Kurti, Paul Oeller
Publikováno v:
The Plant Journal. 33:793-800
This report describes a method for the easy generation of inverted repeat constructs for the silencing of genes of unknown sequence which is applicable to high-throughput studies. This improved procedure for high-efficiency gene silencing is specific
Publikováno v:
Plant Molecular Biology. 50:345-355
The function of the ripening-related endo-1,4-β-D-glucanase (EGase) CaCel1 in fruit softening was investigated by suppression of CaCel1 gene expression in transgenic pepper (Capsicum annuum L.) plants using constitutive expression of a truncated sen
Publikováno v:
Plant Molecular Biology. 50:357-369
The ripening-related pepper endo-1,4-β-D-glucanase (EGase) CaCel1 was over-expressed in transgenic tomato plants under the control of the constitutive 35S promoter to investigate the effects on plant growth and fruit softening of high levels of a po
Autor:
David A. Brummell, Mark H. Harpster
Publikováno v:
Plant Molecular Biology. 47:311-339
Excessive softening is the main factor limiting fruit shelf life and storage. Transgenic plants modified in the expression of cell wall modifying proteins have been used to investigate the role of particular activities in fruit softening during ripen