Zobrazeno 1 - 10
of 208
pro vyhledávání: '"Mark C Leake"'
Autor:
Adam JM Wollman, Sviatlana Shashkova, Erik G Hedlund, Rosmarie Friemann, Stefan Hohmann, Mark C Leake
Publikováno v:
eLife, Vol 8 (2019)
Externí odkaz:
https://doaj.org/article/cc2758e872644534a57b41aedc54452f
Autor:
Victoria A Lund, Katarzyna Wacnik, Robert D Turner, Bryony E Cotterell, Christa G Walther, Samuel J Fenn, Fabian Grein, Adam JM Wollman, Mark C Leake, Nicolas Olivier, Ashley Cadby, Stéphane Mesnage, Simon Jones, Simon J Foster
Publikováno v:
eLife, Vol 7 (2018)
The bacterial cell wall is essential for viability, but despite its ability to withstand internal turgor must remain dynamic to permit growth and division. Peptidoglycan is the major cell wall structural polymer, whose synthesis requires multiple int
Externí odkaz:
https://doaj.org/article/e7bf4c1659a047a0972561a7ebc7c0c9
Publikováno v:
Bio-Protocol, Vol 8, Iss 2 (2018)
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before the
Externí odkaz:
https://doaj.org/article/4ed997ab2db24d3eb8a1a41cd98f89f5
Autor:
Adam JM Wollman, Sviatlana Shashkova, Erik G Hedlund, Rosmarie Friemann, Stefan Hohmann, Mark C Leake
Publikováno v:
eLife, Vol 6 (2017)
Transcription is regulated through binding factors to gene promoters to activate or repress expression, however, the mechanisms by which factors find targets remain unclear. Using single-molecule fluorescence microscopy, we determined in vivo stoichi
Externí odkaz:
https://doaj.org/article/2d1389e7eb224ba9b980cd7ee56e54a3
Autor:
Thomas R Beattie, Nitin Kapadia, Emilien Nicolas, Stephan Uphoff, Adam JM Wollman, Mark C Leake, Rodrigo Reyes-Lamothe
Publikováno v:
eLife, Vol 6 (2017)
The replisome is a multiprotein machine that carries out DNA replication. In Escherichia coli, a single pair of replisomes is responsible for duplicating the entire 4.6 Mbp circular chromosome. In vitro studies of reconstituted E. coli replisomes hav
Externí odkaz:
https://doaj.org/article/8c4da7c5d9c146bbb1eeaeb0b9daa265
Autor:
Dominique C.S. Evans, Amanda B. Khamas, Alex Payne-Dwyer, Adam J.M. Wollman, Kristian S. Rasmussen, Janne K. Klitgaard, Birgitte Kallipolitis, Mark C. Leake, Rikke L. Meyer
Publikováno v:
Biofilm, Vol 8, Iss , Pp 100233- (2024)
The major human pathogen Staphylococcus aureus forms biofilms comprising of a fibrin network that increases attachment to surfaces and shields bacteria from the immune system. It secretes two coagulases, Coagulase (Coa) and von Willebrand factor bind
Externí odkaz:
https://doaj.org/article/d950255ce3c94156a393ac185af24026
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 20, Iss , Pp 5264-5274 (2022)
The integration host factor (IHF) is a prominent example of indirect readout as it imposes one of the strongest bends on relaxed linear DNA. However, the relation between IHF and torsionally constrained DNA, as occurs physiologically, remains unclear
Externí odkaz:
https://doaj.org/article/04956f119e9247879e540c6867ee53d7
Publikováno v:
iScience, Vol 25, Iss 12, Pp 105675- (2022)
Summary: The ability of tumors to establish a pro-tumorigenic microenvironment is an important point of investigation in the search for new therapeutics. Tumors form microenvironments in part by the “education” of immune cells attracted via chemo
Externí odkaz:
https://doaj.org/article/c95b28ec4d9a499896bfde69dec8b93e
Autor:
Mark C. Leake
Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of th
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 19, Iss , Pp 4049-4058 (2021)
As camera pixel arrays have grown larger and faster, and optical microscopy techniques ever more refined, there has been an explosion in the quantity of data acquired during routine light microscopy. At the single-molecule level, analysis involves mu
Externí odkaz:
https://doaj.org/article/e818b14a467e4b4d9e57f197a09aa9ce