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pro vyhledávání: '"Mark C Gebhard"'
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1319
There is significant interest in engineering proteases with desired proteolytic properties. We describe a high-throughput fluorescence-activated cell sorting (FACS) assay for detecting altered proteolytic activity of protease in yeast, at the single
Publikováno v:
Methods in Molecular Biology ISBN: 9781493927470
There is significant interest in engineering proteases with desired proteolytic properties. We describe a high-throughput fluorescence-activated cell sorting (FACS) assay for detecting altered proteolytic activity of protease in yeast, at the single
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ba51ab403925b4bf741c1342089ffeb5
https://doi.org/10.1007/978-1-4939-2748-7_5
https://doi.org/10.1007/978-1-4939-2748-7_5
Myriad new applications of proteases would be enabled by an ability to fine-tune substrate specificity and activity. Herein we present a general strategy for engineering protease selectivity and activity by capitalizing on sequestration of the protea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cfc7da1ac09a23882c5175457b35adf
https://europepmc.org/articles/PMC3645551/
https://europepmc.org/articles/PMC3645551/
Publikováno v:
The FASEB Journal. 27
Autor:
Panagiotis A Konstantinopoulos, Elena Fountzilas, Jeffrey D Goldsmith, Manoj Bhasin, Kamana Pillay, Nancy Francoeur, Towia A Libermann, Mark C Gebhardt, Dimitrios Spentzos
Publikováno v:
PLoS ONE, Vol 5, Iss 4, p e9747 (2010)
BACKGROUND: Diagnosis of soft tissue sarcomas (STS) is challenging. Many remain unclassified (not-otherwise-specified, NOS) or grouped in controversial categories such as malignant fibrous histiocytoma (MFH), with unclear therapeutic value. We analyz
Externí odkaz:
https://doaj.org/article/1287b21eeb1b4f89a0bf4c17e502a4e7