Zobrazeno 1 - 10
of 90
pro vyhledávání: '"Mark Akeson"'
CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma
Autor:
S. John Liu, Martina Malatesta, Brian V. Lien, Parna Saha, Shivani S. Thombare, Sung Jun Hong, Leslie Pedraza, Mark Koontz, Kyounghee Seo, Max A. Horlbeck, Daniel He, Harjus S. Birk, Miten Jain, Hugh E. Olsen, Mark Akeson, Jonathan S. Weissman, Michelle Monje, Nalin Gupta, David R. Raleigh, Erik M. Ullian, Daniel A. Lim
Publikováno v:
Genome Biology, Vol 21, Iss 1, Pp 1-18 (2020)
Abstract Background Long non-coding RNAs (lncRNAs) exhibit highly cell type-specific expression and function, making this class of transcript attractive for targeted cancer therapy. However, the vast majority of lncRNAs have not been tested as potent
Externí odkaz:
https://doaj.org/article/158146c58ea3458da92657b2d5201345
Autor:
Elektra K Robinson, Pratibha Jagannatha, Sergio Covarrubias, Matthew Cattle, Valeriya Smaliy, Rojin Safavi, Barbara Shapleigh, Robin Abu-Shumays, Miten Jain, Suzanne M Cloonan, Mark Akeson, Angela N Brooks, Susan Carpenter
Publikováno v:
eLife, Vol 10 (2021)
Determining the layers of gene regulation within the innate immune response is critical to our understanding of the cellular responses to infection and dysregulation in disease. We identified a conserved mechanism of gene regulation in human and mous
Externí odkaz:
https://doaj.org/article/7623ad82df924ce0aad8a2be411d8d62
Autor:
Ashley Byrne, Anna E. Beaudin, Hugh E. Olsen, Miten Jain, Charles Cole, Theron Palmer, Rebecca M. DuBois, E. Camilla Forsberg, Mark Akeson, Christopher Vollmers
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Short-read RNA-seq is limited in its ability to resolve complex transcript isoforms since it cannot sequence full-length cDNA. Here the authors use Oxford Nanopore MinION and their Mandalorion analysis pipeline to measure complex isoforms in B1a cell
Externí odkaz:
https://doaj.org/article/47e55c463ee54d15b838dd82d9d1d77f
Publikováno v:
PLoS ONE, Vol 14, Iss 5, p e0216709 (2019)
The ribosome small subunit is expressed in all living cells. It performs numerous essential functions during translation, including formation of the initiation complex and proofreading of base-pairs between mRNA codons and tRNA anticodons. The core c
Externí odkaz:
https://doaj.org/article/9e5c8a1867784e43bb43a743fc1b13d6
Autor:
Sarah Stahl-Rommel, Miten Jain, Hang N. Nguyen, Richard R. Arnold, Serena M. Aunon-Chancellor, Gretta Marie Sharp, Christian L. Castro, Kristen K. John, Sissel Juul, Daniel J. Turner, David Stoddart, Benedict Paten, Mark Akeson, Aaron S. Burton, Sarah L. Castro-Wallace
Publikováno v:
Genes, Vol 12, Iss 1, p 106 (2021)
For the past two decades, microbial monitoring of the International Space Station (ISS) has relied on culture-dependent methods that require return to Earth for analysis. This has a number of limitations, with the most significant being bias towards
Externí odkaz:
https://doaj.org/article/22561776fb7445ccbcf7524d26d702aa
Autor:
Aaron S. Burton, Sarah E. Stahl, Kristen K. John, Miten Jain, Sissel Juul, Daniel J. Turner, Eoghan D. Harrington, David Stoddart, Benedict Paten, Mark Akeson, Sarah L. Castro-Wallace
Publikováno v:
Genes, Vol 11, Iss 1, p 76 (2020)
The MinION sequencer has made in situ sequencing feasible in remote locations. Following our initial demonstration of its high performance off planet with Earth-prepared samples, we developed and tested an end-to-end, sample-to-sequencer process that
Externí odkaz:
https://doaj.org/article/a7be82469b724b7482b8d4d7d66fbeba
Publikováno v:
ACS Nano. 15:16642-16653
We describe a method for direct tRNA sequencing using the Oxford Nanopore MinION. The principal technical advance is custom adapters that facilitate end-to-end sequencing of individual transfer RNA (tRNA) molecules at subnanometer precision. A second
Publikováno v:
RNA (New York, N.Y.), vol 27, iss 12
Understanding transcriptomes requires documenting the structures, modifications, and abundances of RNAs as well as their proximity to other molecules. The methods that make this possible depend critically on enzymes (including mutant derivatives) tha
Publikováno v:
Nature methods. 19(10)
Autor:
Camilla Ugolini, Logan Mulroney, Adrien Leger, Matteo Castelli, Elena Criscuolo, Maia Kavanagh Williamson, Andrew D Davidson, Abdulaziz Almuqrin, Roberto Giambruno, Miten Jain, Gianmaria Frigè, Hugh Olsen, George Tzertzinis, Ira Schildkraut, Madalee G Wulf, Ivan R Corrêa, Laurence Ettwiller, Nicola Clementi, Massimo Clementi, Nicasio Mancini, Ewan Birney, Mark Akeson, Francesco Nicassio, David A Matthews, Tommaso Leonardi
Publikováno v:
Ugolini, C, Mulroney, L, Leger, A, Castelli, M, Criscuolo, E, Kavanagh Williamson, M, Davidson, A D, Almuqrin, A M, Giambruno, R, Jain, M, Frige, G, Olsen, H, Tzertzinis, G, Schildkraut, I, Wulf, M G, Corrêa Jr, I R, Ettwiller, L, Clementi, N, Clementi, M, Mancini, N, Birney, E, Akeson, M, Nicassio, F, Matthews, D A & Leonardi, T 2022, ' Nanopore ReCappable sequencing maps SARS-CoV-2 5′ capping sites and provides new insights into the structure of sgRNAs ', Nucleic Acids Research, vol. 50, no. 6, gkac144, pp. 3475-3489 . https://doi.org/10.1093/nar/gkac144
The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested subgenomic RNAsused to express structural and accessory proteins. Long-read sequencing technologies such as nanopore direct RNA sequencing can recover full-length tran