Quantitative co-expression of proteins at the single cell level--application to a multimeric FRET sensor.

Autor: Joachim Goedhart, Laura van Weeren, Merel J W Adjobo-Hermans, Ies Elzenaar, Mark A Hink, Theodorus W J Gadella

Publikováno v: PLoS ONE, Vol 6, Iss 11, p e27321 (2011)

BACKGROUND: Co-expression of proteins is generally achieved by introducing two (or more) independent plasmids into cells, each driving the expression of a different protein of interest. However, the relative expression levels may vary strongly betwee

Externí odkaz: https://doaj.org/article/b4af9cd9a1fb4c00a7d31d143907ddba

Dynamics of Germinosome Formation and FRET-Based Analysis of Interactions between GerD and Germinant Receptor Subunits in Bacillus cereus Spores

Autor: Yan Wang, Ronald M. P. Breedijk, Mark A. Hink, Lars Bults, Norbert O. E. Vischer, Peter Setlow, Stanley Brul

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 20, p 11230 (2021)

Spores of the bacterium Bacillus cereus can cause disease in humans due to contamination of raw materials for food manufacturing. These dormant, resistant spores can survive for years in the environment, but can germinate and grow when their surround

Externí odkaz: https://doaj.org/article/73d4d0561e0d4a20bae2e9a670752770

Temporal tracking of quantum-dot apatite across in vitro mycorrhizal networks shows how host demand can influence fungal nutrient transfer strategies

Autor: E. Toby Kiers, Loreto Oyarte Gálvez, Malin Klein, Marten Postma, Thomas S. Shimizu, Anouk van ’t Padje, Mark A. Hink

Publikováno v: ISME Journal, 15(2), 435-449. Nature Publishing Group
The ISME Journal, 15(2). Nature Publishing Group
The ISME Journal
ISME Journal 15 (2021)
ISME Journal, 15, 435-449
van’t Padje, A, Oyarte Galvez, L, Klein, M, Hink, M A, Postma, M, Shimizu, T & Kiers, E T 2021, ' Temporal tracking of quantum-dot apatite across in vitro mycorrhizal networks shows how host demand can influence fungal nutrient transfer strategies ', ISME Journal, vol. 15, no. 2, pp. 435-449 . https://doi.org/10.1038/s41396-020-00786-w

Arbuscular mycorrhizal fungi function as conduits for underground nutrient transport. While the fungal partner is dependent on the plant host for its carbon (C) needs, the amount of nutrients that the fungus allocates to hosts can vary with context.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bd22f2f10fa2851a9cca1d8dcd5d3fa
https://doi.org/10.1038/s41396-020-00786-w

Erratum for Meiresonne et al., 'Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by

Autor: Nils Y, Meiresonne, René, van der Ploeg, Mark A, Hink, Tanneke, den Blaauwen

Publikováno v: mBio

One of the mechanisms of β-lactam antibiotic resistance requires the activity of d,d-carboxypeptidases (d,d-CPases) involved in peptidoglycan (PG) synthesis, making them putative targets for new antibiotic development. The activity of PG-synthesizin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5d9c219922a7bda8730e477031fb7e4e
https://pubmed.ncbi.nlm.nih.gov/28900026

Quantitative live-cell imaging and computational modelling shed new light on endogenous WNT/CTNNB1 signaling dynamics

Autor: Tanne van der Wal, Gooitzen Zwanenburg, Renée van Amerongen, Saskia Ma de Man, Mark A. Hink

Publikováno v: eLife, 10:e66440. eLife Sciences Publications
eLife

WNT/CTNNB1 signaling regulates tissue development and homeostasis in all multicellular animals, but the underlying molecular mechanism remains incompletely understood. Specifically, quantitative insight into endogenous protein behavior is missing. He

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::200d6034fc4d2af1dbf13cd48a6fef6b
https://dare.uva.nl/personal/pure/en/publications/quantitative-livecell-imaging-and-computational-modelling-shed-new-light-on-endogenous-wntctnnb1-signaling-dynamics(680250d0-be55-4e65-beea-191099940b0b).html