Výsledky vyhledávání - "Marit E, Zwierzina"

Akademický článek

The circadian transcription factor ARNTL2 is regulated by weight-loss interventions in human white adipose tissue and inhibits adipogenesis

Autor: Markus Mandl, Hans P. Viertler, Maria Zopoglou, Maria C. Mitterberger-Vogt, Juliane Gasser, Florian M. Hatzmann, Tina Rauchenwald, Marit E. Zwierzina, Monika Mattesich, Alexander K. H. Weiss, Lorenza Mottes, Camille Brucker, Petra Waldegger, Gerhard Pierer, Werner Zwerschke

Publikováno v: Cell Death Discovery, Vol 8, Iss 1, Pp 1-12 (2022)

Abstract Misalignment of physiological circadian rhythms promotes obesity which is characterized by white adipose tissue (WAT) expansion. Differentiation of Adipose stem/progenitor cells (ASCs) contributes to WAT increase but the importance of the ce

Externí odkaz: https://doaj.org/article/66e38723e5c74f4fbeb7f4f6b0631e28

Zobrazit plný text záznamu

Akademický článek

CRISPR/Cas9-mediated gene knockout in human adipose stem/progenitor cells

Autor: Markus Mandl, Heike Ritthammer, Asim Ejaz, Sonja A. Wagner, Florian M. Hatzmann, Saphira Baumgarten, Hans P. Viertler, Marit E. Zwierzina, Monika Mattesich, Valerie Schiller, Tina Rauchenwald, Christian Ploner, Petra Waldegger, Gerhard Pierer, Werner Zwerschke

Publikováno v: Adipocyte, Vol 9, Iss 1, Pp 626-635 (2020)

The CRISPR/Cas9 system is a powerful tool to generate a specific loss-of-function phenotype by gene knockout (KO). However, this approach is challenging in primary human cells. In this technical report, we present a reliable protocol to achieve a fun

Externí odkaz: https://doaj.org/article/ea96581b76fa40a48032f2164c93487a

Zobrazit plný text záznamu

Akademický článek

CD146 (MCAM) in human cs-DLK1−/cs-CD34+ adipose stromal/progenitor cells

Autor: Susanne Hörl, Asim Ejaz, Sebastian Ernst, Monika Mattesich, Andreas Kaiser, Brigitte Jenewein, Marit E. Zwierzina, Sarina Hammerle, Carina Miggitsch, Maria C. Mitterberger-Vogt, Claudia Krautgasser, Gerhard Pierer, Werner Zwerschke

Publikováno v: Stem Cell Research, Vol 22, Iss C, Pp 1-12 (2017)

To precisely characterize CD146 in adipose stromal/progenitor cells (ASCs) we sorted the stromal vascular faction (SVF) of human abdominal subcutaneous white adipose tissue (sWAT) according to cell surface (cs) expression of CD146, DLK1 and CD34. Thi

Externí odkaz: https://doaj.org/article/4d1408babe0e4eeeb9f8a8c6d9093084

Zobrazit plný text záznamu

Akademický článek

Characterization of DLK1(PREF1)+/CD34+ cells in vascular stroma of human white adipose tissue

Autor: Marit E. Zwierzina, Asim Ejaz, Mario Bitsche, Michael J.F. Blumer, Maria C. Mitterberger, Monika Mattesich, Arno Amann, Andreas Kaiser, Elisabeth J. Pechriggl, Susanne Hörl, Ursula Rostek, Gerhard Pierer, Helga Fritsch, Werner Zwerschke

Publikováno v: Stem Cell Research, Vol 15, Iss 2, Pp 403-418 (2015)

Sorting of native (unpermeabilized) SVF-cells from human subcutaneous (s)WAT for cell surface staining (cs) of DLK1 and CD34 identified three main populations: ~10% stained cs-DLK1+/cs-CD34−, ~20% cs-DLK1+/cs-CD34+dim and ~45% cs-DLK1−/cs-CD34+.

Externí odkaz: https://doaj.org/article/b53be5860fad4c0da8bb6a672ba5a7da

Zobrazit plný text záznamu