Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce

Autor: Flavia Soledad Darqui, Laura Mabel Radonic, Valeria Cecilia Beracochea, H. Esteban Hopp, Marisa López Bilbao

Publikováno v: Frontiers in Plant Science, Vol 12 (2021)

The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represent

Externí odkaz: https://doaj.org/article/824d95050e3f41e3b5d358043d6bc031

CRISPR/Cas9-mediated knockout of SPL13 radically increases lettuce yield

Autor: Valeria Beracochea, Margarita Stritzler, Laura Radonic, Emilia Bottero, Cintia Jozefkowicz, Flavia Darqui, Nicolás Ayub, Marisa López Bilbao, Gabriela Soto

Publikováno v: Plant Cell Reports. 42:645-647

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5a4198bca49c46ba35f4b7fd00d41b0
https://doi.org/10.1007/s00299-022-02952-0

The ALS Gene as Genetic Target in CRISPR/ Cas Approaches: What Have We Learned So Far?

Autor: Flavia Soledad Darqui, Marisa López Bilbao, Horacio Esteban Hopp

Publikováno v: Modern Concepts & Developments in Agronomy 7 (2) : 700-711 (Agosto 2020)
INTA Digital (INTA)
Instituto Nacional de Tecnología Agropecuaria
instacron:INTA

Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cc59d8ee177d824f6d1657cc22087b9
https://doi.org/10.31031/mcda.2020.07.000656

Simplified methodology for large scale isolation of homozygous transgenic lines of lettuce

Autor: Flavia Soledad Darqui, H. Esteban Hopp, Marisa López Bilbao, Nilda López, Laura M. Radonic

Publikováno v: Electronic Journal of Biotechnology 31 : 1-9 (January 2018)
INTA Digital (INTA)
Instituto Nacional de Tecnología Agropecuaria
instacron:INTA
Electronic Journal of Biotechnology, Vol 31, Iss C, Pp 1-9 (2018)

Background: Lettuce is a globally important leafy vegetable and a model plant for biotechnology due to its adaptability to tissue culture and stable genetic transformation. Lettuce is also crucial for functional genomics research in the Asteraceae wh

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bfa04ae32c982f2856f24c374ce457b
https://www.sciencedirect.com/science/article/pii/S0717345817300611

Sunflower (Helianthus annuus L.)

Autor: Laura M, Radonic, Dalia M, Lewi, Nilda E, López, H Esteban, Hopp, Alejandro S, Escandón, Marisa López, Bilbao

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1224

Sunflower (Helianthus annuus L.) is still considered as a recalcitrant species to in vitro culture and transformation in spite of the publication of different protocols. Here we describe a routine transformation system of this crop which requires mat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::cf341624565c5c911fcd3b79b87d3af4
https://pubmed.ncbi.nlm.nih.gov/25416248