Fast and scalable purification of a therapeutic full‐length antibody based on process crystallization

Autor: Bernhard Helk, Matthias Mechelke, Dariusch Hekmat, Marion Giffard, Benjamin Smejkal, Neeraj Jagdish Agrawal, Philipp Heckmeier, Franziska Ortner, Henk Schulz, Bernhardt L. Trout

Publikováno v: Biotechnology and Bioengineering. 110:2452-2461

The potential of process crystallization for purification of a therapeutic monoclonal IgG1 antibody was studied. The purified antibody was crystallized in non-agitated micro-batch experiments for the first time. A direct crystallization from clarifie

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ff813f25f46fd23f542d2b3583525a5
https://doi.org/10.1002/bit.24908

Surfactant Poloxamer 188 as a New Crystallizing Agent for Urate Oxidase

Autor: Vanessa Delfosse, Bertrand Castro, Françoise Bonneté, Claudine Mayer, Marion Giffard, Giuliano Sciara, Mohamed El Hajji, Christian Cambillau

Publikováno v: Crystal Growth & Design
Crystal Growth & Design, 2009, 9 (9), pp.4199-4206
Crystal Growth & Design, American Chemical Society, 2009, 9 (9), pp.4199-4206. ⟨10.1021/cg900473n⟩
Crystal Growth & Design, American Chemical Society, 2009, 9 (9), pp.4199-4206
HAL
Crystal Growth & Design, 2009, 9 (9), pp.4199-4206. ⟨10.1021/cg900473n⟩
Crystal Growth and Design
Crystal Growth and Design, American Chemical Society, 2009, 9 (9), pp.4199-4206. ⟨10.1021/cg900473n⟩

International audience; We characterize a new class of crystallizing agent for soluble protein crystallization compatible with both pharmaceutical processes and high-resolution structure determination in biocrystallography. Poloxamers are amphiphilic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f1f1f2b9ac644e6ed9ef729e172fce2
https://doi.org/10.1021/cg900473n

High-throughput phase diagram mapping of urate oxidase via powder diffraction

Autor: Richard A. Kahn, Françoise Bonneté, Jonathan P. Wright, Sotonye Dagogo, Ines E. Collings, Irene Margiolaki, Yves Watier, Andrew N. Fitch, Marion Giffard

Publikováno v: Acta Crystallographica Section a Foundations of Crystallography
Acta Crystallographica Section a Foundations of Crystallography, Wiley-Blackwell, 2009, 65 (a1), pp.s321-s322. ⟨10.1107/s0108767309093143⟩
Acta Crystallographica Section A : Foundations of Crystallography [1968-2013]
Acta Crystallographica Section A : Foundations of Crystallography [1968-2013], 2009, 65 (a1), pp.s321-s322. ⟨10.1107/s0108767309093143⟩

International audience

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3881aece7fd09fd7d13caa4d301df928
https://hal.archives-ouvertes.fr/hal-02549526

Salting-In Effects on Urate Oxidase Crystal Design

Autor: Nathalie Colloc’h, Françoise Bonneté, Marion Giffard, Bertrand Castro, Natalie Ferté

Publikováno v: Crystal Growth & Design
Crystal Growth & Design, American Chemical Society, 2008, 8 (12), pp.4220-4226
Crystal Growth & Design, 2008, 8 (12), pp.4220-4226

In this paper, solubility and interactions in solution of the recombinant urate oxidase from Aspergillus flavus, rasburicase, are studied both in the absence and in the presence of salt at a pH close to the pI. An intense salting-in effect is demonst

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ddd12c1f7aabbbe3851e9b17adb3086
https://hal.archives-ouvertes.fr/hal-00386894