Výsledky vyhledávání - "Marilyn Fukushima"

Akademický článek

DMSO and betaine greatly improve amplification of GC-rich constructs in de novo synthesis.

Autor: Michael A Jensen, Marilyn Fukushima, Ronald W Davis

Publikováno v: PLoS ONE, Vol 5, Iss 6, p e11024 (2010)

In Synthetic Biology, de novo synthesis of GC-rich constructs poses a major challenge because of secondary structure formation and mispriming. While there are many web-based tools for codon optimizing difficult regions, no method currently exists tha

Externí odkaz: https://doaj.org/article/505a4e8eecf9401b8d5e87419f55f775

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Allelic resolution NGS HLA typing of Class I and Class II loci and haplotypes in Cape Town, South Africa

Autor: Ming Li, Virginie Rozot, Yvonne R. Thorstenson, Michael N. Mindrinos, Mark M. Davis, Farbod Babrzadeh, Tracy T. Nguyen, Chunlin Wang, Raquel Kuehn, Marcelo A. Fernandez Viña, Thomas J. Scriba, Huang Huang, Sujatha Krishnakumar, Sandeep Kancharla, Marilyn Fukushima, Lisa E. Creary

Publikováno v: Human Immunology. 79:839-847

The development of next-generation sequencing (NGS) methods for HLA genotyping has already had an impact on the scope and precision of HLA research. In this study, allelic resolution HLA typing was obtained for 402 individuals from Cape Town, South A

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c00f8d30b330a2847b546d05ace2846
https://doi.org/10.1016/j.humimm.2018.09.004

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Targeted and Highly Multiplexed Detection of Microorganisms by Employing an Ensemble of Molecular Probes

Autor: Marilyn Fukushima, Sujatha Krishnakumar, Molly Miranda, Robert P. St.Onge, Ronald W. Davis, Eula Fung, Richard W. Hyman, Curtis J. Palm, Weihong Xu, Michael A. Jensen

Publikováno v: Applied and Environmental Microbiology. 80:4153-4161

The vast majority of microscopic life on earth consists of microbes that do not grow in laboratory culture. To profile the microbial diversity in environmental and clinical samples, we have devised and employed molecular probe technology, which detec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::260443cf59db745004621b86e8346ce3
https://doi.org/10.1128/aem.00666-14

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Diversity of the Vaginal Microbiome Correlates With Preterm Birth

Autor: Joan F. Hilton, Kim Chi Vo, Larry Rand, Linda C. Giudice, Marilyn Fukushima, Richard W. Hyman, Aaron B. Caughey, Ronald W. Davis, Brittni Johnson, Eula Fung, Hui Jiang

Publikováno v: Reproductive sciences (Thousand Oaks, Calif.), vol 21, iss 1
Hyman, RW; Fukushima, M; Jiang, H; Fung, E; Rand, L; Johnson, B; et al.(2014). Diversity of the vaginal microbiome correlates with preterm birth. Reproductive Sciences, 21(1), 32-40. doi: 10.1177/1933719113488838. UCSF: Retrieved from: http://www.escholarship.org/uc/item/2nn7r4nc

Reproductive tract infection is a major initiator of preterm birth (PTB). The objective of this prospective cohort study of 88 participants was to determine whether PTB correlates with the vaginal microbiome during pregnancy. Total DNA was purified f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::897aeced8664d5eabb5f3dc81c5445c6
https://doi.org/10.1177/1933719113488838

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CHANGES IN THE HUMAN SKIN MICROBIOME OVER ONE YEARâS TIME

Autor: Curtis J. Palm, Marilyn Fukushima, Ronald W. Davis, Richard W. Hyman, Farbod Babrzadeh, Chunlin Wang

Publikováno v: American Journal of Microbiology. 3:18-30

Human skin comprises a large number of distinguishable ec ological niches. To describe fully the human skin microbiome, it will be necessary to identify t he bacteria in each niche and to distinguish the co mmensal bacteria from the temporary residen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3c60878754a6026c25c716df4f8d4a47
https://doi.org/10.3844/ajmsp.2012.18.30

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The dynamics of the vaginal microbiome during infertility therapy with in vitro fertilization-embryo transfer

Autor: Marilyn Fukushima, Denise Bernstein, Christopher N. Herndon, Zara Zelenko, Ronald W. Davis, Hui Jiang, Linda C. Giudice, Curtis J. Palm, Kim Chi Vo, Richard W. Hyman

Publikováno v: Journal of Assisted Reproduction and Genetics. 29:105-115

To determine the vaginal microbiome in women undergoing IVF-ET and investigate correlations with clinical outcomes.Thirty patients had blood drawn for estradiol (E(2)) and progesterone (P(4)) at four time points during the IVF-ET cycle and at 4-6 wee

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e1d2e6733f6b5b49095e81f588ee5c7
https://doi.org/10.1007/s10815-011-9694-6

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Bacteria on Human Skin

Autor: Richard W. Hyman, Curtis J. Palm, Ronald W. Davis, Farbod Babrzadeh, Marilyn Fukushima, Chunlin Wang

Publikováno v: American Journal of Microbiology. 2:25-34

Problem statement: Human skin is a large (~ 1.75 m2) organ containing a large number of ecologically distinct sites. These sites harbor a variety of distinct microbiomes. One challenge is to define the microbiome at every site. We chose two interesti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1debc155d16d878caa8bd1b237ff1da8
https://doi.org/10.3844/ajmsp.2011.25.34

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Multiplex Identification of Microbes

Autor: Ronald W. Davis, Robert P. St.Onge, Marilyn Fukushima, Molly Miranda, Richard W. Hyman, Edward A. Allen, Ana Aparicio

Publikováno v: Applied and Environmental Microbiology. 76:3904-3910

We have adapted molecular inversion probe technology to identify microbes in a highly multiplexed procedure. This procedure does not require growth of the microbes. Rather, the technology employs DNA homology twice: once for the molecular probe to hy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e745ea4dde697c21d4961669b59be7c
https://doi.org/10.1128/aem.02785-09

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Microbes on the human vaginal epithelium

Autor: Ronald W. Davis, Marilyn Fukushima, Richard W. Hyman, Jochen Kumm, Linda C. Giudice, Lisa Diamond

Publikováno v: Proceedings of the National Academy of Sciences. 102:7952-7957

Using solely a gene-based procedure, PCR amplification of the 16S ribosomal RNA gene coupled with very deep sequencing of the amplified products, the microbes on 20 human vaginal epithelia of healthy women have been identified and quantitated. The La

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1bf57abcdb459c762ce1851d924f0fe
https://doi.org/10.1073/pnas.0503236102

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Direct oligonucleotide synthesis onto super-paramagnetic beads

Autor: Michael S. Akhras, Marilyn Fukushima, Nader Pourmand, Michael A. Jensen, Ronald W. Davis

Publikováno v: Journal of biotechnology, vol 167, iss 4

Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::873ce205bf9a83f4d7bda2369ec4a8cb
https://escholarship.org/uc/item/2x03z495

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