Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Marija Vrljic"'
Autor:
Jiajie Diao, Patricia Grob, Daniel J Cipriano, Minjoung Kyoung, Yunxiang Zhang, Sachi Shah, Amie Nguyen, Mark Padolina, Ankita Srivastava, Marija Vrljic, Ankita Shah, Eva Nogales, Steven Chu, Axel T Brunger
Publikováno v:
eLife, Vol 4 (2015)
Externí odkaz:
https://doaj.org/article/ee9ef692ee1c4ab4b79608e2cc275b41
Autor:
Jiajie Diao, Patricia Grob, Daniel J Cipriano, Minjoung Kyoung, Yunxiang Zhang, Sachi Shah, Amie Nguyen, Mark Padolina, Ankita Srivastava, Marija Vrljic, Ankita Shah, Eva Nogales, Steven Chu, Axel T Brunger
Publikováno v:
eLife, Vol 1 (2012)
The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle–vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence
Externí odkaz:
https://doaj.org/article/7439d9935d5b41ab9eb3efd1ac2fd2d9
Publikováno v:
Biochemistry
Synaptotagmin 1 (Syt1) is a Ca(2+) sensor for SNARE-mediated, Ca(2+)-triggered synaptic vesicle fusion in neurons. It is composed of luminal, transmembrane, linker, and two Ca(2+)-binding (C2) domains. Here we describe expression and purification of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b11e6e22f4e4b5b625a3dce292dac5d9
http://europepmc.org/articles/PMC3217305
http://europepmc.org/articles/PMC3217305
Publikováno v:
Nature structural & molecular biology
Synchronous neurotransmission is triggered when Ca(2+) binds to synaptotagmin 1 (Syt1), a synaptic-vesicle protein that interacts with SNAREs and membranes. We used single-molecule fluorescence resonance energy transfer (FRET) between synaptotagmin's
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3b7c05211bd24069cc0a012308497d2
https://doi.org/10.1038/nsmb.1763
https://doi.org/10.1038/nsmb.1763
Autor:
Akihiro Kusumi, Yasuhiro Umemura, Takahiro K. Fujiwara, Kenichi G. N. Suzuki, Marija Vrljic, Stefanie Y. Nishimura
Publikováno v:
Biophysical Journal. 95(1):435-450
Previously, investigations using single-fluorescent-molecule tracking at frame rates of up to 65 Hz, showed that the transmembrane MHC class II protein and its GPI-anchored modified form expressed in CHO cells undergo simple Brownian diffusion, witho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8002b0d1ffcfe7a616b7a2704d05ba8
The Structure of the Yeast Plasma Membrane SNARE Complex Reveals Destabilizing Water-filled Cavities
Publikováno v:
Journal of Biological Chemistry. 283:1113-1119
SNARE proteins form a complex that leads to membrane fusion between vesicles, organelles, and plasma membrane in all eukaryotic cells. We report the 1.7A resolution structure of the SNARE complex that mediates exocytosis at the plasma membrane in the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e335381e388b356e9155b11d177691d
https://doi.org/10.1074/jbc.m707912200
https://doi.org/10.1074/jbc.m707912200
Publikováno v:
Biophysical Journal. 90:927-938
Glycosylphosphatidylinositol-linked and transmembrane major histocompatibility complex (MHC) class II I-E(k) proteins, as well as N-(6-tetramethylrhodaminethiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (Tritc-DHPE), are used as pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb1bed478569af127060d3d6375b5606
https://doi.org/10.1529/biophysj.105.070524
https://doi.org/10.1529/biophysj.105.070524
Publikováno v:
Biophysical Journal. 88(1):334-347
Glycosylphosphatidylinositol (GPI)-linked and native major histocompatibility complex class II I-E(k) were used as probes to determine the effect of varying cholesterol concentration on the mobility of proteins in the plasma membrane. These proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee447d6dc5c100433a8b52367a20573a
Autor:
Harden M. McConnell, Marija Vrljic
Publikováno v:
Annual Review of Biophysics and Biomolecular Structure. 32:469-492
▪ Abstract The observation of liquid-liquid immiscibility in cholesterol-phospholipid mixtures in monolayers and bilayers has opened a broad field of research into their physical chemistry. Some mixtures exhibit multiple immiscibilities. This unusu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::035912595da3ff37d1be4af79682f9d7
https://doi.org/10.1146/annurev.biophys.32.110601.141704
https://doi.org/10.1146/annurev.biophys.32.110601.141704
Publikováno v:
Biophysical Journal. 83(5):2681-2692
Single-molecule epifluorescence microscopy was used to observe the translational motion of GPI-linked and native I-E(k) class II MHC membrane proteins in the plasma membrane of CHO cells. The purpose of the study was to look for deviations from Brown
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df268a7f625ed3dbde0ed53c5aa9de42