High throughput screening and identification of coagulopathic snake venom proteins and peptides using nanofractionation and proteomics approaches.

Autor: Julien Slagboom, Marija Mladić, Chunfang Xie, Taline D Kazandjian, Freek Vonk, Govert W Somsen, Nicholas R Casewell, Jeroen Kool

Publikováno v: PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0007802 (2020)

Snakebite is a neglected tropical disease that results in a variety of systemic and local pathologies in envenomed victims and is responsible for around 138,000 deaths every year. Many snake venoms cause severe coagulopathy that makes victims vulnera

Externí odkaz: https://doaj.org/article/db84340e92474074a8a0fa671ebe61e4

Detection and identification of antibacterial proteins in snake venoms using at-line nanofractionation coupled to LC-MS

Autor: Julien Slagboom, Gilles P. van Wezel, Jeroen Kool, Marija Mladic, Freek J. Vonk, Michael K. Richardson

Publikováno v: Toxicon, 155, 66-74. PERGAMON-ELSEVIER SCIENCE LTD
Mladic, M, Slagboom, J, Kool, J, Vonk, F J, van Wezel, G P & Richardson, M K 2018, ' Detection and identification of antibacterial proteins in snake venoms using at-line nanofractionation coupled to LC-MS ', Toxicon, vol. 155, pp. 66-74 . https://doi.org/10.1016/j.toxicon.2018.08.009
Toxicon, 155, 66-74. Elsevier Limited

This study describes the application of at-line nanofractionation to the screening of snake venoms for antibacterial activity against Gram-positive and Gram-negative bacteria, the detection of proteins of interest, and their partial or full identific

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::816aa28945c3fc72f974a8913cbdcf78
https://doi.org/10.1016/j.toxicon.2018.08.009

At-Line Cellular Screening Methodology for Bioactives in Mixtures Targeting the α7-Nicotinic Acetylcholine Receptor

Autor: Gabriela Arias-Alpizar, Jeroen Kool, Wilfried M. A. Niessen, August B. Smit, Marija Mladic, Reka A. Otvos, Govert W. Somsen

Publikováno v: SLAS Discovery. 21:459-467

The α7-nicotinic acetylcholine receptor (α7-nAChR) is a ligand-gated ion channel expressed in different regions of the central nervous system (CNS). The α7-nAChR has been associated with Alzheimer's disease, epilepsy, and schizophrenia, and theref

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bd952303d58f486d27b0ba50a05b2ad
https://doi.org/10.1177/1087057115625307

Metabolic profiling of ligands for the chemokine receptor CXCR3 by liquid chromatography-mass spectrometry coupled to bioaffinity assessment

Autor: David Falck, Maikel Wijtmans, Martine J. Smit, Rob Leurs, Jeroen Kool, Danny J. Scholten, Marija Mladic, Wilfried M. A. Niessen

Publikováno v: Analytical and Bioanalytical Chemistry, 407(23), 7067-81. Analytical and Bioanalytical Chemistry
Mladic, M, Scholten, D J, Wijtmans, M, Falck, D, Leurs, R, Niessen, W M A, Smit, M J & Kool, J 2015, ' Metabolic profiling of ligands for the chemokine receptor CXCR3 by liquid chromatography-mass spectrometry coupled to bioaffinity assessment ', Analytical and Bioanalytical Chemistry, vol. 407, no. 23, pp. 7067-81 . https://doi.org/10.1007/s00216-015-8867-z
Analytical and Bioanalytical Chemistry, 407(23), 7067-7081
Analytical and Bioanalytical Chemistry

Chemokine receptors belong to the class of G protein-coupled receptors and are important in the host defense against infections and inflammation. However, aberrant chemokine signaling is linked to different disorders such as cancer, central nervous s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::680602957da63a1aa59f82c4df40b072
https://hdl.handle.net/1871.1/6645112a-8ab2-4805-9f4a-87e5b18543b2

Rapid screening and identification of ACE inhibitors in snake venoms using at-line nanofractionation LC-MS

Autor: Lindsey Burggraaff, Govert W. Somsen, Julien Slagboom, Marija Mladic, Wilfried M. A. Niessen, R. Manjunatha Kini, Jeroen Kool, Tessa de Waal

Publikováno v: Mladic, M, de Waal, T, Burggraaff, L, Slagboom, J, Somsen, G W, Niessen, W M A, Kini, R M & Kool, J 2017, ' Rapid screening and identification of ACE inhibitors in snake venoms using at-line nanofractionation LC-MS ', Analytical and Bioanalytical Chemistry, vol. 409, no. 25, pp. 5987-5997 . https://doi.org/10.1007/s00216-017-0531-3
Analytical and Bioanalytical Chemistry, 409(25), 5987-5997. Analytical and Bioanalytical Chemistry
Analytical and Bioanalytical Chemistry

This study presents an analytical method for the screening of snake venoms for inhibitors of the angiotensin-converting enzyme (ACE) and a strategy for their rapid identification. The method is based on an at-line nanofractionation approach, which co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db349b06abcd9b182c45743171c42e8c
https://hdl.handle.net/1871.1/55953182-d00f-4b83-ab13-ea9e03e006fd