Advanced Fluorescent Polymer Probes for the Site-Specific Labeling of Proteins in Live Cells Using the HaloTag Technology

Autor: Thomas Berki, Anush Bakunts, Damien Duret, Laura Fabre, Catherine Ladavière, Andrea Orsi, Marie-Thérèse Charreyre, Andrea Raimondi, Eelco van Anken, Arnaud Favier

Publikováno v: ACS Omega, Vol 4, Iss 7, Pp 12841-12847 (2019)

Externí odkaz: https://doaj.org/article/f9d33a9fb29245b19753f38f593544a9

Labeling of native proteins with fluorescent RAFT polymer probes: application to the detection of a cell surface protein using flow cytometry

Autor: Philippe Bouvet, Yann Leverrier, T. Berki, Marie-Thérèse Charreyre, M. Carretier, Zofia Haftek-Terreau, Damien Duret, Jacqueline Marvel, Catherine Ladavière, Arnaud Favier, K. Monier

Publikováno v: Polymer Chemistry. 9:1857-1868

Conjugation of fluorescent polymer chains synthesized by RAFT controlled radical polymerization with native proteins was achieved by introducing an activated ester function at their ω-chain-end via a very efficient thiol–ene click post-polymerizat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c2e78bc1378438e4273b80349cb0e080
https://doi.org/10.1039/c7py02064c

Innovative particle standards and long-lived imaging for 2D and 3D dSTORM

Autor: Angelina Provost, Corentin Rousset, Laura Bourdon, Sarra Mezhoud, Emma Reungoat, Camille Fourneaux, Timothée Bresson, Marine Pauly, Nicolas Béard, Laura Possi-Tchouanlong, Boyan Grigorov, Philippe Bouvet, Jean-Jacques Diaz, Christophe Chamot, Eve-Isabelle Pécheur, Catherine Ladavière, Marie-Thérèse Charreyre, Arnaud Favier, Christophe Place, Karine Monier

Publikováno v: Scientific Reports
Scientific Reports, Nature Publishing Group, 2019, 9 (1), ⟨10.1038/s41598-019-53528-0⟩
Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.17967. ⟨10.1038/s41598-019-53528-0⟩
Scientific Reports, 2019, 9 (1), pp.17967. ⟨10.1038/s41598-019-53528-0⟩
Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)

International audience; Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional micro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::156e9a2e00aa763fd4551b02a60f7c20
https://hal.archives-ouvertes.fr/hal-02387253

Fluorescent gold nanoparticles with chain-end grafted RAFT copolymers: influence of the polymer molecular weight and type of chromophore

Autor: Yann Leverrier, Cécile Chamignon, Cristina Cepraga, Cyrille Monnereau, Arnaud Favier, Sophie Marotte, Chantal Andraud, Pierre-Henri Lanoë, Pierre Alcouffe, Frédéric Lerouge, Edna Ben Daoud, Jacqueline Marvel, Marie-Thérèse Charreyre, Stephane Parola

Publikováno v: Polymer Chemistry
Polymer Chemistry, Royal Society of Chemistry-RSC, 2016, 7, pp.6812-6825. ⟨10.1039/C6PY01625A⟩
Polymer Chemistry, 2016, 7, pp.6812-6825. ⟨10.1039/C6PY01625A⟩

International audience; Fluorescent gold nanoparticles (GNPs) were prepared by chain-end grafting of RAFT copolymers bearing multiple fluorophores distributed along the chain. Two different synthetic approaches in water were first studied with well-d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01bec32909ea6b12f009c343df0ad9be
https://doi.org/10.1039/c6py01625a

'Polymultivalent' Polymer-Peptide Cluster Conjugates for an Enhanced Targeting of Cells Expressing α

Autor: Damien, Duret, Adrien, Grassin, Maxime, Henry, Thibault, Jacquet, Fabien, Thoreau, Sandrine, Denis-Quanquin, Jean-Luc, Coll, Didier, Boturyn, Arnaud, Favier, Marie-Thérèse, Charreyre

Publikováno v: Bioconjugate chemistry. 28(9)

A new class of "polymultivalent" ligands combining several ligand clusters and a water-soluble biocompatible polymer is introduced. These original conjugates bear two levels of multivalency. They are prepared by covalent coupling of a controlled numb

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::652e83dcbad08f2fc306ca63478305d8
https://pubmed.ncbi.nlm.nih.gov/28767233