An engineered PET depolymerase to break down and recycle plastic bottles

Autor: B. David, Michel Chateau, Christopher M. Topham, M. Cot, E. Guémard, Vincent Tournier, E. Kamionka, A. Gilles, Gianluca Cioci, M. Dalibey, Elisabeth Moya-Leclair, C. Folgoas, Sophie Barbe, Alain Marty, Marie-Laure Desrousseaux, S. Gavalda, J. Nomme, Sophie Duquesne, Isabelle André, Helene Texier

Publikováno v: Nature
Nature, Nature Publishing Group, 2020, 580 (7802), pp.216-219. ⟨10.1038/s41586-020-2149-4⟩
Nature, 2020, 580 (7802), pp.216-219. ⟨10.1038/s41586-020-2149-4⟩

Present estimates suggest that of the 359 million tons of plastics produced annually worldwide1, 150–200 million tons accumulate in landfill or in the natural environment2. Poly(ethylene terephthalate) (PET) is the most abundant polyester plastic,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c83c2b04ebf907a18a0a32992c7aa3bd
https://doi.org/10.1038/s41586-020-2149-4

A 1H NMR study of the specificity of α-l-arabinofuranosidases on natural and unnatural substrates

Autor: Vinciane Borsenberger, Emmie Dornez, Stéphane Massou, Marie-Laure Desrousseaux, Michael J. O’Donohue, Régis Fauré, Claire Dumon, Maija Tenkanen, Christophe M. Courtin

Publikováno v: Biochimica et Biophysica Acta (BBA)-General Subjects
Biochimica et Biophysica Acta (BBA)-General Subjects, Elsevier, 2014, 1840 (10), pp.3106-3114. ⟨10.1016/j.bbagen.2014.07.001⟩
Biochimica et Biophysica Acta (BBA)-General Subjects, 2014, 1840 (10), pp.3106-3114. ⟨10.1016/j.bbagen.2014.07.001⟩

Background The detailed characterization of arabinoxylan-active enzymes, such as double-substituted xylan arabinofuranosidase activity, is still a challenging topic. Ad hoc chromogenic substrates are useful tools and can reveal subtle differences in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e88b6699c210f052d7d5e234f0b6f86b
https://hal.insa-toulouse.fr/hal-02146175

A substrate for the detection of broad specificity alpha-L-arabinofuranosidases with indirect release of a chromogenic group

Autor: Vinciane Borsenberger, Marie-Laure Desrousseaux, Régis Fauré, Emmie Dornez, Michael J. O’Donohue, Christophe M. Courtin

Publikováno v: Tetrahedron Letters
Tetrahedron Letters, Elsevier, 2013, 54 (24), pp.3063-3066. ⟨10.1016/j.tetlet.2013.03.136⟩
Tetrahedron Letters, 2013, 54 (24), pp.3063-3066. ⟨10.1016/j.tetlet.2013.03.136⟩

The synthesis of a compound containing a 4-nitrocatechol bound to two vicinal alpha-L-arabinofuranosyl moieties through a linker arm was achieved using a sulfate protecting group to facilitate selective alkylation of one aromatic hydroxyl. Several al

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd450fb5653ddc42941453c349023ee5
https://hal.archives-ouvertes.fr/hal-01268100

The GH51 α-l-arabinofuranosidase from Paenibacillus sp. THS1 is multifunctional, hydrolyzing main-chain and side-chain glycosidic bonds in heteroxylans

Autor: Mohamed Manai, Caroline Rémond, Hanen Bouraoui, Narcis Fernandez-Fuentes, Eleni Ioannou, Claire Dumon, Michael J. O’Donohue, Pablo Alvira, Marie-Laure Desrousseaux

Publikováno v: Biotechnology for Biofuels
Biotechnology for Biofuels, BioMed Central, 2016, 9 (1), pp.1-14. ⟨10.1186/s13068-016-0550-x⟩
Biotechnology for Biofuels 1 (9), 1-14. (2016)
Biotechnology for Biofuels, 2016, 9 (1), pp.1-14. ⟨10.1186/s13068-016-0550-x⟩

Background Conceptually, multi-functional enzymes are attractive because in the case of complex polymer hydrolysis having two or more activities defined by a single enzyme offers the possibility of synergy and reduced enzyme cocktail complexity. Neve

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24e2e1621600ad5588b5e746cb858d2d