Transfer of mesenchymal stem cell mitochondria to CD4+ T cells contributes to repress Th1 differentiation by downregulating T-bet expression

Autor: Waseem Akhter, Jean Nakhle, Loïc Vaillant, Geneviève Garcin, Cécile Le Saout, Matthieu Simon, Carole Crozet, Farida Djouad, Christian Jorgensen, Marie-Luce Vignais, Javier Hernandez

Publikováno v: Stem Cell Research & Therapy, Vol 14, Iss 1, Pp 1-16 (2023)

Abstract Background Mesenchymal stem/stromal cells (MSCs) are multipotent cells with strong tissue repair and immunomodulatory properties. Due to their ability to repress pathogenic immune responses, and in particular T cell responses, they show ther

Externí odkaz: https://doaj.org/article/c2887cd5e244455180eb189946c64c6d

Transfer of Cardiac Mitochondria Improves the Therapeutic Efficacy of Mesenchymal Stem Cells in a Preclinical Model of Ischemic Heart Disease

Autor: Marie-Luce Vignais, Jennyfer Levoux, Pierre Sicard, Khattar Khattar, Catherine Lozza, Marianne Gervais, Safia Mezhoud, Jean Nakhle, Frederic Relaix, Onnik Agbulut, Jeremy Fauconnier, Anne-Marie Rodriguez

Publikováno v: Cells, Vol 12, Iss 4, p 582 (2023)

Background: The use of mesenchymal stem cells (MSCs) appears to be a promising therapeutic approach for cardiac repair after myocardial infarction. However, clinical trials have revealed the need to improve their therapeutic efficacy. Recent evidence

Externí odkaz: https://doaj.org/article/f8d01c54ff974646bb0e21bf16e5fa7d

Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring

Autor: Jean Nakhle, Khattar Khattar, Tülin Özkan, Adel Boughlita, Daouda Abba Moussa, Amelie Darlix, Frédérique Lorcy, Valérie RIGAU, Luc BAUCHET, Sabine Gerbal-Chaloin, Martine Daujat-Chavanieu, Floriant Bellvert, Laurent Turchi, Thierry Virolle, Jean-Philippe Hugnot, Nicolas Buisine, Mireille Galloni, Valerie Dardalhon, Anne-Marie Rodriguez, Marie-Luce Vignais

Publikováno v: Cancer Research Communications
Cancer Research Communications, 2023, 3 (6), pp.1041-1056. ⟨10.1158/2767-9764.CRC-23-0144⟩
Cancer Research Communications, 2023, 3, pp.1041-1056. ⟨10.1158/2767-9764.crc-23-0144⟩

International audience; Glioblastomas (GBM) are heterogeneous tumors with high metabolic plasticity. Their poor prognosis is linked to the presence of glioblastoma stem cells (GSC), which support resistance to therapy, notably to temozolomide (TMZ).

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a82ca59d992aa68ebf144b2b728720ed
https://hal.science/hal-04154698

Supplementary Materials and Methods from Mitochondria transfer from mesenchymal stem cells confers chemoresistance to Glioblastoma stem cells through metabolic rewiring

Autor: Marie-Luce Vignais, Anne-Marie Rodriguez, Valerie Dardalhon, Mireille Galloni, Nicolas Buisine, Jean-Philippe Hugnot, Thierry Virolle, Laurent Turchi, Floriant Bellvert, Martine Daujat-Chavanieu, Sabine Gerbal-Chaloin, Luc BAUCHET, Valérie RIGAU, Frédérique Lorcy, Amelie Darlix, Daouda Abba Moussa, Adel Boughlita, Tülin Özkan, Khattar Khattar, Jean Nakhle

TMZ dose-response assay Caspase-GloⓇ 3/7 assay Cytotox assay Extracellular Flux Assays Cell Proliferation Western Blot Flow Cytometry Metabolite usage Mitoplates Mass Spectrometry Quantification 13C stable isotope tracing experiments Preparation an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5721f3708ee485a20a9e29d39d09e8b7
https://doi.org/10.1158/2767-9764.23192200.v1

Data from Mitochondria transfer from mesenchymal stem cells confers chemoresistance to Glioblastoma stem cells through metabolic rewiring

Autor: Marie-Luce Vignais, Anne-Marie Rodriguez, Valerie Dardalhon, Mireille Galloni, Nicolas Buisine, Jean-Philippe Hugnot, Thierry Virolle, Laurent Turchi, Floriant Bellvert, Martine Daujat-Chavanieu, Sabine Gerbal-Chaloin, Luc BAUCHET, Valérie RIGAU, Frédérique Lorcy, Amelie Darlix, Daouda Abba Moussa, Adel Boughlita, Tülin Özkan, Khattar Khattar, Jean Nakhle

Glioblastomas (GBM) are heterogeneous tumors with high metabolic plasticity. Their poor prognosis is linked to the presence of glioblastoma stem cells (GSCs), which support resistance to therapy, notably to temozolomide (TMZ). Mesenchymal stem cells

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3920829ce074ee71aeddfb94bb7003f0
https://doi.org/10.1158/2767-9764.c.6664816.v1