Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Marie C. Ruiz-Martinez"'
Autor:
Darin R. Latimer, James A. Ball, John W. Simpson, Greg T. Went, Jonathan M. Rothberg, Jan Berka, Marie C. Ruiz-Martinez, Greg T. Mulhern
Publikováno v:
Electrophoresis. 21:135-149
In this paper we present the development of a DNA analysis system using a microfabricated channel device and a novel transmission imaging spectrograph which can be efficiently incorporated into a high throughput genomics facility for both sizing and
Publikováno v:
Analytical Chemistry. 70:1516-1527
A method for the cleanup of Sanger DNA sequencing reaction products for capillary electrophoresis analysis with replaceable polymer solutions has been developed. A poly(ether sulfone) ultrafiltration membrane pretreated with linear polyacrylamide was
Autor:
Wolfgang Goetzinger, Igor P. Smirnov, Arthur W. Miller, David J. Brady, Marie C. Ruiz-Martinez, Barry L. Karger, Emanuel Carrilho, Jan Berka
Publikováno v:
Analytical Chemistry. 68:3305-3313
The read length for DNA sequencing using capillary electrophoresis and replaceable linear polyacrylamide (LPA) solutions has been extended to more than 1000 bases with a run time of 80 min. This result was successfully achieved through the combined u
Publikováno v:
Analytical chemistry. 70(8)
In the previous paper, a sample cleanup procedure for DNA sequencing reaction products was developed, in which template DNA was removed by ultrafiltration and the total concentration of salts (chloride and di- and deoxynucleotides) was decreased belo
Autor:
Emanuel Carrilho, Oscar Salas-Solano, Wolfgang Goetzinger, Lev Kotler, Barry L. Karger, Marie C. Ruiz-Martinez
Publikováno v:
Electrophoresis. 19(2)
In a previous paper, a 2% w/w replaceable high molecular mass linear polyacrylamide solution (high molecular mass LPA) was used to achieve long read-lengths for DNA sequencing by capillary electrophoresis (E. Carrilho et al., Anal. Chem. 1996, 68, 33
Autor:
Lev Kotler, Jeffrey Kesilman, Marie C. Ruiz-Martinez, Emanuel Carrilho, Barry L. Karger, Arthur W. Miller
Publikováno v:
SPIE Proceedings.
Factors to be considered for robust high-throughput automated dna sequencing using a multiple-capillary array instrumentEmanuel Carri1ho, Arthur W. Miller, Marie C. RuizMartinezt, Lev Kotler, Jeffrey Kesilman, and Barry L. Karger*Northeastern Univers
Autor:
Barry L. Karger, Marie C. Ruiz-Martinez, Emanuel Carrilho, J. Kieleczawa, S. Carson, Arthur W. Miller, František Foret, Jan Berka
Publikováno v:
ResearcherID
Two strategies for DNA sequencing by primer walking using short oligonucleotide primer libraries have been successfully employed along with capillary electrophoresis using replaceable polymer solutions of linear polyacrylamide and fluorescence detect
Autor:
Aharon S. Cohen, Jan Berka, Barry L. Karger, S. Carson, Alexei. Belenkii, Marie C. Ruiz-Martinez
Publikováno v:
Analytical chemistry. 65(22)
This paper presents the principles of an instrument designed for DNA sequencing using the standard four-dye-labeled primer approach. The method is based on capillary electrophoresis with laser-induced fluorescence and an intensified diode array detec
Autor:
Arthur W. Miller, František Foret, Barry L. Karger, Jan Berka, Alexei. Belenkii, Marie C. Ruiz-Martinez
Publikováno v:
Analytical chemistry. 65(20)
Replaceable linear polyacrylamide (LPA) has been utilized as a sieving matrix for DNA sequencing by capillary electrophoresis (CE). Difficulties associated with cross-linked polyacrylamide gel stability have been overcome for the routine application