Zobrazeno 1 - 10
of 83
pro vyhledávání: '"Marialuisa, Zerbini"'
Autor:
Donatella Santini, Daniela Barbieri, Marialuisa Zerbini, M. Nocera, Elisa Leo, Simona Venturoli, Silvano Costa, Monica Cricca
Publikováno v:
Journal of Clinical Virology. 53:121-124
Background The Abbott RealTi m e High Risk HPV assay (ART) is an automated multiplex real-time PCR test for detection of DNA from 14 high risk (HR) HPV types in cervical specimens and simultaneous distinction of HPV16 and HPV18 from other HR-HPV. Obj
Autor:
Marialuisa Zerbini, Nunzio Salfi, Chiara Puccetti, Brunella Guerra, Francesca Bonvicini, Giorgio Gallinella, Nicola Rizzo
Publikováno v:
Journal of Clinical Microbiology. 49:3514-3518
Parvovirus B19 infection during pregnancy is a potential hazard to the fetus because of the virus' ability to infect fetal erythroid precursor cells and fetal tissues. Fetal complications range from transitory fetal anemia and nonimmune fetal hydrops
Functional analysis and quantitative determination of the expression profile of human parvovirus B19
Autor:
Elisabetta Manaresi, Claudia Filippone, Francesca Bonvicini, Marialuisa Zerbini, Monica Musiani, Giorgio Gallinella
Publikováno v:
Virology. 381(2):168-177
Comprehension of the pathogenetic potential of human parvovirus B19 requires the definition of the complete spectrum of cellular tropism and a functional analysis of the viral genome in infected cells. In this study, we carried out a systematic funct
Autor:
Elisa Leo, Simone Ambretti, Monica Cricca, Silvano Costa, Monica Musiani, Simona Venturoli, Marialuisa Zerbini
Publikováno v:
Journal of Medical Virology. 80:1434-1440
The evidence on genotype-specific risk in women infected with human papillomavirus (HPV) with normal cytology and the importance of the distinction of high-risk (HR)-HPV genotypes in the management of low-grade lesions suggest that the distinction of
Peptide Nucleic Acid–Based In Situ Hybridization Assay for Detection of Parvovirus B19 Nucleic Acids
Autor:
Elisabetta Manaresi, Giovanna Angela Gentilomi, Marialuisa Zerbini, Giorgio Gallinella, Claudia Filippone, Monica Musiani, Francesca Bonvicini
Publikováno v:
Clinical Chemistry. 52:973-978
Background: Peptide nucleic acid (PNA) molecules are known to bind complementary nucleic acid sequences with a much stronger affinity and with more stable binding than DNA or RNA molecules. We chose parvovirus B19, which is diagnosed by detection of
Autor:
Marialuisa Zerbini, Claudia Filippone, Francesca Bonvicini, Elisabetta Manaresi, Monica Musiani, Stefania Delbarba, Giorgio Gallinella
Publikováno v:
Virology. 347:447-454
The variation in the amount of parvovirus B19 DNA and different classes of RNA in permissive and non-permissive infected cells was analysed by means of quantitative real-time PCR and RT-PCR assays. In the permissive bone marrow mononuclear cells, UT7
Autor:
Marialuisa Zerbini, Alberta Azzi, R. DeSantis, Elisabetta Manaresi, Monica Musiani, K. Zakrzewska
Publikováno v:
Journal of Medical Virology. 72:679-682
Parvovirus B19 infection occurs very frequently in patients with haemophilia on account of its transmission with plasma derivatives. In order to achieve a more defined serological pattern for the study of the role of B19 infection in haemophilic arth
Efficient treatment of paraffin-embedded cervical tissue for HPV DNA testing by HC-II and PCR assays
Autor:
Francesca Bonvicini, Monica Musiani, Simone Ambretti, Giovanna Angela Gentilomi, Simona Venturoli, Monica Cricca, Marialuisa Zerbini, Giorgio Gallinella
Publikováno v:
Journal of Clinical Virology. 29:137-140
Background and objectives: High-risk human papillomaviruses (HR-HPVs) are the primary cause of cervical cancer. In order to meet with clinical requirements, a direct capture test with signal amplification (HC-II), able to detect the 13 prevalent HR-H
Autor:
Giovanna Gentilomi, Lisa Zeri, Severino Ghini, Elisa Zuffi, Stefano Girotti, Marialuisa Zerbini, Monica Musiani
Publikováno v:
Luminescence. 18:207-213
In this study we describe an efficient stable genetic transformation of the phytopathogenic bacterium Erwinia amylovora using a recombinant expression vector encoding the firefly luciferase gene of Photinus pyralis, which is further controlled by IPT
Autor:
Giorgio Gallinella, Giovanna Gentilomi, Monica Cricca, Monica Musiani, Marialuisa Zerbini, Simona Venturoli
Publikováno v:
Clinica Chimica Acta. 302:79-87
Highly sensitive assay systems are necessary for large-scale virological screenings. We evaluated the use of tyramide signal amplification (TSA) for biotinylated probe in dot-blot hybridization assay to detect B19 DNA in serum samples. The probe was