Plasma membrane H+-ATPases sustain pollen tube growth and fertilization

Autor: Robert D. Hoffmann, Maria Teresa Portes, Lene Irene Olsen, Daniel Santa Cruz Damineli, Maki Hayashi, Custódio O. Nunes, Jesper T. Pedersen, Pedro T. Lima, Cláudia Campos, José A. Feijó, Michael Palmgren

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020)

Cytosolic ion gradients in growing pollen tubes are thought to be required for polar growth. Here the authors show that the Arabidopsis plasma membrane H+ ATPases, AHA6, AHA8, and AHA9, maintain tip-to-shank proton gradients, oscillations in cytosoli

Externí odkaz: https://doaj.org/article/19bd39f44f6b4640a9a224a9ee668f06

Measuring Extracellular Proton and Anionic Fluxes in Arabidopsis Pollen Tubes

Autor: Maria Teresa Portes, José Feijó

Publikováno v: Bio-Protocol, Vol 11, Iss 3 (2021)

The ion-selective vibrating probe has been used to detect and quantify the magnitude and direction of transmembrane fluxes of several ions in a wide range of biological systems. Inherently non-invasive, vibrating probes have been essential to access

Externí odkaz: https://doaj.org/article/6e8fcf3ef2814343b6457a1cc750e166

Electrifying rhythms in plant cells

Autor: Daniel S.C. Damineli, Maria Teresa Portes, José A. Feijó

Publikováno v: Curr Opin Cell Biol
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Physiological oscillations (or rhythms) pervade all spatiotemporal scales of biological organization, either because they perform critical functions or simply because they can arise spontaneously and may be difficult to prevent. Regardless of the cas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16bf96e3f675183aed90441d1c445130
https://pubmed.ncbi.nlm.nih.gov/35809387

Measuring Extracellular Proton and Anionic Fluxes in Arabidopsis Pollen Tubes

Autor: José A. Feijó, Maria Teresa Portes

Publikováno v: BIO-PROTOCOL. 11

The ion-selective vibrating probe has been used to detect and quantify the magnitude and direction of transmembrane fluxes of several ions in a wide range of biological systems. Inherently non-invasive, vibrating probes have been essential to access

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d0ec59e21cc70e7e3a9a5e8fb0dd45f2
https://doi.org/10.21769/bioprotoc.3908

Plasma membrane H+-ATPases sustain pollen tube growth and fertilization

Autor: Pedro Lima, Custódio Oliveira Nunes, Jesper Torbøl Pedersen, Maria Teresa Portes, Michael G. Palmgren, Lene Irene Olsen, Daniel S. C. Damineli, José A. Feijó, Maki Hayashi, Claudia Guimarães Camargo Campos, Robert D. Hoffmann

Publikováno v: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
Hoffmann, R D, Portes, M T, Olsen, L I, Damineli, D S C, Hayashi, M, Nunes, C O, Pedersen, J T, Lima, P T, Campos, C, Feijó, J A & Palmgren, M 2020, ' Plasma membrane H +-ATPases sustain pollen tube growth and fertilization ', Nature Communications, vol. 11, no. 1, 2395 . https://doi.org/10.1038/s41467-020-16253-1

Pollen tubes are highly polarized tip-growing cells that depend on cytosolic pH gradients for signaling and growth. Autoinhibited plasma membrane proton (H+) ATPases (AHAs) have been proposed to energize pollen tube growth and underlie cell polarity,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9a43ff7f857ee585b099fc8f6ee7c89
https://doi.org/10.1038/s41467-020-16253-1