GenoMine: a CRISPR-Cas9-based kill switch for biocontainment of Pseudomonas putida

Autor: Enrique Asin-Garcia, Maria Martin-Pascual, Claudia de Buck, Max Allewijn, Alexandra Müller, Vitor A. P. Martins dos Santos

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)

Synthetic genetic circuits have revolutionised our capacity to control cell viability by conferring microorganisms with programmable functionalities to limit survival to specific environmental conditions. Here, we present the GenoMine safeguard, a CR

Externí odkaz: https://doaj.org/article/b3281f6fb79b4276a41246913351885d

SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, BioBricks and Type IIS restriction enzyme standards

Autor: Stamatios G. Damalas, Christos Batianis, Maria Martin‐Pascual, Victor deLorenzo, Vitor A. P. Martins dos Santos

Publikováno v: Microbial Biotechnology, Vol 13, Iss 6, Pp 1793-1806 (2020)

Summary Robust synthetic biology applications rely heavily on the design and assembly of DNA parts with specific functionalities based on engineering principles. However, the assembly standards adopted by different communities vary considerably, thus

Externí odkaz: https://doaj.org/article/5748065e788943498a2c31f69221222f

Breaking the Restriction Barriers and Applying CRISPRi as a Gene Silencing Tool in Pseudoclostridium thermosuccinogenes

Autor: Joyshree Ganguly, Maria Martin-Pascual, Diego Montiel González, Alkan Bulut, Bram Vermeulen, Ivo Tjalma, Athina Vidaki, Richard van Kranenburg

Publikováno v: Microorganisms, Vol 10, Iss 4, p 698 (2022)

Pseudoclostridium thermosuccinogenes is a thermophilic bacterium capable of producing succinate from lignocellulosic-derived sugars and has the potential to be exploited as a platform organism. However, exploitation of P. thermosuccinogenes has been

Externí odkaz: https://doaj.org/article/96075e0800de4ba38326a983f086d370

ReScribe: An Unrestrained Tool Combining Multiplex Recombineering and Minimal-PAM ScCas9 for Genome Recoding Pseudomonas putida

Autor: Luis Garcia-Morales, Maria Martin-Pascual, Richard van Kranenburg, Enrique Asin-Garcia, Vitor A. P. Martins dos Santos

Publikováno v: ACS synthetic biology, 10(10), 2672-2688
ACS synthetic biology 10 (2021) 10

Genome recoding enables incorporating new functions into the DNA of microorganisms. By reassigning codons to noncanonical amino acids, the generation of new-to-nature proteins offers countless opportunities for bioproduction and biocontainment in ind

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fe50ee5d8772d803f33f52c10ec7199
https://research.wur.nl/en/publications/rescribe-an-unrestrained-tool-combining-multiplex-recombineering-

An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida

Autor: Maria Martin-Pascual, Pablo I. Nikel, Daniel C. Volke, Stamatios G. Damalas, Ekaterina Kozaeva, Christos Batianis, Vitor A. P. Martins dos Santos

Publikováno v: Microbial Biotechnology 13 (2020) 2
Microbial Biotechnology, Vol 13, Iss 2, Pp 368-385 (2020)
Microbial Biotechnology
Microbial Biotechnology, 13(2), 368-385
Batianis, C, Kozaeva, E, Damalas, S G, Martín-Pascual, M, Volke, D C, Nikel, P I & Martins dos Santos, V A P 2020, ' An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida ', Microbial Biotechnology, vol. 13, no. 2, pp. 368-385 . https://doi.org/10.1111/1751-7915.13533

Summary Owing to its wide metabolic versatility and physiological robustness, together with amenability to genetic manipulations and high resistance to stressful conditions, Pseudomonas putida is increasingly becoming the organism of choice for a ran

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79ba8f723e6c11a35fb9f31be7cba666
https://research.wur.nl/en/publications/an-expanded-crispri-toolbox-for-tunable-control-of-gene-expressio