Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes

Autor: Renata S, Tavares, Sara, Escada-Rebelo, Maria M, Soares, Andreia F, Silva, Teresa, Almeida-Santos, Sandra, Amaral, Ana Paula, Sousa, João, Ramalho-Santos

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2310

A positive relationship between mitochondrial functionality and gamete quality, ultimately contributing to fertilization success and normal embryo development has been established for some years now. Both mitochondrial membrane potential (MMP) and re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::eaf97198954d5f325ffb9650e5f1347c
https://pubmed.ncbi.nlm.nih.gov/34095998

Chemical reversion of age-related oocyte dysfunction fails to enhance embryo development in a bovine model of postovulatory aging.

Autor: Ferreira AF; Reproductive Medicine Unit, Gynecology, Obstetrics, Reproduction and Neonatology Department, Unidade Local de Saúde de Coimbra, Praceta, R. Prof. Mota Pinto, Coimbra, 3004-561, Portugal. filipaferreira44@gmail.com.; Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, Coimbra, 3000-548, Portugal. filipaferreira44@gmail.com.; CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal. filipaferreira44@gmail.com.; CIBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal. filipaferreira44@gmail.com.; EUGIN Coimbra, Filipe Hodart N° 12, 3000-185, Coimbra, Portugal. filipaferreira44@gmail.com., Machado-Simões J; Institute for Interdisciplinary Research, Doctoral Programme in Experimental Biology and Biomedicine (PDBEB), University of Coimbra, Coimbra, Portugal., Moniz I; CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; Institute for Interdisciplinary Research, Doctoral Programme in Experimental Biology and Biomedicine (PDBEB), University of Coimbra, Coimbra, Portugal., Soares M; CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; Institute for Interdisciplinary Research, Doctoral Programme in Experimental Biology and Biomedicine (PDBEB), University of Coimbra, Coimbra, Portugal., Carvalho A; Reproductive Medicine Unit, Gynecology, Obstetrics, Reproduction and Neonatology Department, Unidade Local de Saúde de Coimbra, Praceta, R. Prof. Mota Pinto, Coimbra, 3004-561, Portugal.; CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal., Diniz P; CIISA - Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477, Lisbon, Portugal., Ramalho-Santos J; CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; Department of Life Sciences, Calçada Martim de Freitas, University of Coimbra, 3000-456, Coimbra, Portugal., Sousa AP; Reproductive Medicine Unit, Gynecology, Obstetrics, Reproduction and Neonatology Department, Unidade Local de Saúde de Coimbra, Praceta, R. Prof. Mota Pinto, Coimbra, 3004-561, Portugal.; CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; EUGIN Coimbra, Filipe Hodart N° 12, 3000-185, Coimbra, Portugal., Lopes-da-Costa L; CIISA - Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477, Lisbon, Portugal.; AL4AnimalS - Associate Laboratory for Animal and Veterinary Science, Avenida da Universidade Técnica, 1300-477, Lisbon, Portugal., Almeida-Santos T; Reproductive Medicine Unit, Gynecology, Obstetrics, Reproduction and Neonatology Department, Unidade Local de Saúde de Coimbra, Praceta, R. Prof. Mota Pinto, Coimbra, 3004-561, Portugal.; Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba, Celas, Coimbra, 3000-548, Portugal.; CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; CIBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal.; EUGIN Coimbra, Filipe Hodart N° 12, 3000-185, Coimbra, Portugal.

Publikováno v: Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2024 Aug; Vol. 41 (8), pp. 1997-2009. Date of Electronic Publication: 2024 Jun 01.

Optimization of Platelet-Rich Plasma Preparation for Regenerative Medicine: Comparison of Different Anticoagulants and Resuspension Media.

Autor: Carvalho A; Reproductive Medicine Unit, Gynecology, Obstetrics, Reproduction and Neonatology Department, Centro Hospitalar e Universitário de Coimbra, 3004-561 Coimbra, Portugal.; CICS-UBI-Health Sciences Research Centre, University of Beira Interior, 6200-506 Covilhã, Portugal.; CNC-UC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal., Ferreira AF; Reproductive Medicine Unit, Gynecology, Obstetrics, Reproduction and Neonatology Department, Centro Hospitalar e Universitário de Coimbra, 3004-561 Coimbra, Portugal.; CNC-UC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.; Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal., Soares M; CNC-UC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.; Doctoral Programme in Experimental Biology and Biomedicine (PDBEB), Institute for Interdisciplinary Research, University of Coimbra, 3000-456 Coimbra, Portugal., Santos S; Flow Cytometry Unit, Clinical Pathology Department, Centro Hospitalar e Universitário de Coimbra EPE, 3000-075 Coimbra, Portugal., Tomé P; Flow Cytometry Unit, Clinical Pathology Department, Centro Hospitalar e Universitário de Coimbra EPE, 3000-075 Coimbra, Portugal., Machado-Simões J; CNC-UC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.; Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal., Pais AS; Reproductive Medicine Unit, Gynecology, Obstetrics, Reproduction and Neonatology Department, Centro Hospitalar e Universitário de Coimbra, 3004-561 Coimbra, Portugal.; CNC-UC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.; Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal., Sousa AP; Reproductive Medicine Unit, Gynecology, Obstetrics, Reproduction and Neonatology Department, Centro Hospitalar e Universitário de Coimbra, 3004-561 Coimbra, Portugal.; CNC-UC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal., Paiva A; Flow Cytometry Unit, Clinical Pathology Department, Centro Hospitalar e Universitário de Coimbra EPE, 3000-075 Coimbra, Portugal.; Center for Innovative Biomedicine and Biotechnology (CIBB), Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal.; Ciências Biomédicas Laboratoriais, ESTESC-Coimbra Health School, Instituto Politécnico de Coimbra, 3046-854 Coimbra, Portugal., Almeida-Santos T; Reproductive Medicine Unit, Gynecology, Obstetrics, Reproduction and Neonatology Department, Centro Hospitalar e Universitário de Coimbra, 3004-561 Coimbra, Portugal.; CNC-UC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.; Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal.

Publikováno v: Bioengineering (Basel, Switzerland) [Bioengineering (Basel)] 2024 Feb 23; Vol. 11 (3). Date of Electronic Publication: 2024 Feb 23.

Aging and oocyte competence: A molecular cell perspective.

Autor: Ferreira AF; Reproductive Medicine Unit, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal.; Faculty of Medicine, Azinhaga de Santa Comba, University of Coimbra, Coimbra, Portugal.; CNC-Center for Neuroscience and Cell Biology, CIBB, University of Coimbra, Coimbra, Portugal., Soares M; CNC-Center for Neuroscience and Cell Biology, CIBB, University of Coimbra, Coimbra, Portugal.; PhD Programme in Experimental Biology and Biomedicine (PDBEB), Institute for Interdisciplinary Research (IIIUC), University of Coimbra, Coimbra, Portugal., Almeida-Santos T; Reproductive Medicine Unit, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal.; Faculty of Medicine, Azinhaga de Santa Comba, University of Coimbra, Coimbra, Portugal.; CNC-Center for Neuroscience and Cell Biology, CIBB, University of Coimbra, Coimbra, Portugal., Ramalho-Santos J; CNC-Center for Neuroscience and Cell Biology, CIBB, University of Coimbra, Coimbra, Portugal.; Department of Life Sciences, Calçada Martim de Freitas, University of Coimbra, Coimbra, Portugal., Sousa AP; Reproductive Medicine Unit, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal.; CNC-Center for Neuroscience and Cell Biology, CIBB, University of Coimbra, Coimbra, Portugal.

Publikováno v: WIREs mechanisms of disease [WIREs Mech Dis] 2023 Sep-Oct; Vol. 15 (5), pp. e1613. Date of Electronic Publication: 2023 May 29.

Brumadinho dam collapse induces changes in the microbiome and the antibiotic resistance of the Paraopeba River (Minas Gerais, Brazil).

Autor: Thompson C; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil. Electronic address: thompsoncristiane@gmail.com., Garcia G; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil; State UniversityDarcy Ribeiro (UENF), Centro de Biociências e Biotecnologia, Laboratório de Ciências Ambientais, Campos dos Goytacazes, Rio de Janeiro, Brazil., Masi BP; Laboratório de Ecologia Marinha e Oceanografia Pesqueira da Amazônia (LEMOPA), Instituto Socioambiental e dos Recursos Hídricos (ISARH), Universidade Federal Rural da Amazônia (UFRA), Belém, PA 66077-830, Brazil., Freitas T; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Paz PHC; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Leal CV; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Otsuki K; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Tschoeke D; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil; Health Systems Engineering Laboratory, Alberto Luiz Coimbra Institute of Graduate Studies and Engineering Research (COPPE), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Salazar V; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Soares M; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Lopes G; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Bacha L; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Cosenza C; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Vieira VV; Interdisciplinary Laboratory of Medical Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil., Botelho ACN; Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Oliveira BCV; Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Rezende CE; State UniversityDarcy Ribeiro (UENF), Centro de Biociências e Biotecnologia, Laboratório de Ciências Ambientais, Campos dos Goytacazes, Rio de Janeiro, Brazil., Teixeira L; Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Thompson F; Institute of Biology and SAGE/COPPE, Centro de Gestão Tecnológica-CT2, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil. Electronic address: fabianothompson1@gmail.com.

Publikováno v: The Science of the total environment [Sci Total Environ] 2023 Mar 20; Vol. 865, pp. 161278. Date of Electronic Publication: 2022 Dec 30.