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pro vyhledávání: '"Maria Kielanowska"'
Autor:
Maria Kielanowska, David Shugar
Publikováno v:
Nucleic Acids Research. 3:817-824
Poly 2'0-ethylcytidylic acid (poly (Ce)) was prepared by polymerization of 2'-0-ethylcytidine-5'-pyrophosphate with Escherichia coli polynucleotide phosphorylase in the presence of Mn++, and its properties compared with those of poly (rC), poly (Cm)
Publikováno v:
Biochemical and biophysical research communications. 53(2)
2′ (3′)-O-ethyl-CMP was prepared by alkylation of CMP with diethylsulphate in alkaline medium and deaminated to give 2′(3′)-O-ethyl-UMP, which was phosphorylated to 2′(3′)-O-ethyl-UDP. About 90% of the product consisted of the 2′ isomer