Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Maria Erali"'
Autor:
Genevieve Pont-Kingdon, Lan-Szu Chou, Kristy Damjanovich, Kelli Sumner, Mark Herrmann, Maria Erali, Elaine Lyon
Publikováno v:
BioTechniques, Vol 42, Iss 2, Pp 193-197 (2007)
Multiplexing genotyping technologies usually require as many probes as genetic variants. Oligonucleotides that span multiple loci—loci spanning probes (LSProbes)—hybridize to two or more noncontiguous DNA sequences present in a template and can b
Externí odkaz:
https://doaj.org/article/71a00d49ad354f13b2b04833af11ecf7
Autor:
Maria Erali, Carl T. Wittwer
Publikováno v:
Methods. 50:250-261
High resolution melting is a new method of genotyping and variant scanning that can be seamlessly appended to PCR amplification. Limitations of genotyping by amplicon melting can be addressed by unlabeled probe or snapback primer analysis, all perfor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9234aea3b3b0899dfa468fcdfb9e6b9f
https://doi.org/10.1016/j.ymeth.2010.01.013
https://doi.org/10.1016/j.ymeth.2010.01.013
Publikováno v:
The Journal of Molecular Diagnostics. 11:439-445
The INFINITI HPV-QUAD assay is a commercially available genotyping platform for human papillomavirus (HPV) that uses multiplex PCR, followed by automated processing for primer extension, hybridization, and detection. The analytical performance of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a23926f701f0f800f2c15c5b7a33652
https://doi.org/10.2353/jmoldx.2009.080154
https://doi.org/10.2353/jmoldx.2009.080154
Publikováno v:
Experimental and Molecular Pathology. 85:50-58
Separation of the two strands of DNA with heat (melting) is a fundamental property of DNA that is conveniently monitored with fluorescence. Conventional melting is performed after PCR on any real-time instrument to monitor product purity (dsDNA dyes)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::571d1f2421475eb048c76b8f0777b58f
https://doi.org/10.1016/j.yexmp.2008.03.012
https://doi.org/10.1016/j.yexmp.2008.03.012
Autor:
Maria Erali, Carl T. Wittwer, Rebecca L. Margraf, Elaine Lyon, Sheri Mitchell, Lesa Nelson, Rong Mao, Michael Liew
Publikováno v:
The Journal of Molecular Diagnostics. 8:97-104
High-resolution melting techniques are a simple and cost-effective alternative to other closed-tube genotyping methods. Here, we genotyped human platelet antigens (HPAs) 1 to 6 and 15 by high-resolution melting methods that did not require labeled pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::605d465390278f29991fc1b49141ee9f
https://doi.org/10.2353/jmoldx.2006.050053
https://doi.org/10.2353/jmoldx.2006.050053
Autor:
Shale Dames, L. Kathryn Bromley, Roger E. Smith, Marc G. Elgort, Karl V. Voelkerding, Maria Erali, Mark G. Herrmann
Publikováno v:
The Journal of Molecular Diagnostics. 8:16-21
A disposable 0.2-ml polymerase chain reaction (PCR) tube modified with an aluminum oxide membrane (AOM) has been developed for the extraction, amplification, and detection of nucleic acids. To assess the dynamic range of AOM tubes for real-time PCR,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::492085afd26fa15dbd0cfe78173841ae
https://doi.org/10.2353/jmoldx.2006.040398
https://doi.org/10.2353/jmoldx.2006.040398
Publikováno v:
Journal of Clinical Microbiology. 43:596-603
Performance characteristics of the COBAS Amplicor HBV Monitor test (Roche Diagnostics), which measures hepatitis B virus (HBV) DNA quantitatively, were evaluated and compared with the Ultrasensitive HBV Hybrid Capture 2 (HC2; Digene Corporation) assa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::702ef3a4c970a75f27b8928275e2728f
https://doi.org/10.1128/jcm.43.2.596-603.2005
https://doi.org/10.1128/jcm.43.2.596-603.2005
Autor:
Karl V. Voelkerding, Roger E. Smith, Maria Erali, Jacob D. Durtschi, Marc G. Elgort, Mark G. Herrmann
Publikováno v:
Journal of Membrane Science. 248:81-87
An optically improved fluid filter for localization and microscopic imaging was prepared by electroless deposition of nickel–boron (Ni–B) nanoparticle structures in the approximately 0.2 um channel walls of alumina-based Anopore™ fluid filter m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a334338d77f7ae20f9dda9c9a00a5d93
https://doi.org/10.1016/j.memsci.2004.09.024
https://doi.org/10.1016/j.memsci.2004.09.024
Publikováno v:
Journal of Clinical Microbiology. 42:4545-4551
The genotype of the infecting hepatitis C virus (HCV) helps determine the patient's prognosis and the duration of treatment. Heteroduplex mobility analysis (HMA) is a rapid, inexpensive method for genotyping of HCV that does not require sequencing. W
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b98cbbf5d6441b4b27f020a50e30ac77
https://doi.org/10.1128/jcm.42.10.4545-4551.2004
https://doi.org/10.1128/jcm.42.10.4545-4551.2004
Publikováno v:
Clinical Chemistry. 49:732-739
Background: Genetic risk factors associated with venous thrombosis include mutations in the factor V (Leiden), factor II (prothrombin), and methylenetetrahydrofolate reductase (MTHFR) genes. We evaluated a method using electronically addressable micr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::163de82916ab5e6f2cedce8609a64098
https://doi.org/10.1373/49.5.732
https://doi.org/10.1373/49.5.732