Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Maria Candida M Mellado"'
Autor:
António Roldão, Maria Candida M Mellado, J C Lima, Manuel J T Carrondo, Paula M Alves, R Oliveira
Publikováno v:
PLoS Computational Biology, Vol 8, Iss 2, p e1002367 (2012)
Previous studies have reported the production of malformed virus-like-particles (VLP) in recombinant host systems. Here we computationally investigate the case of a large triple-layered rotavirus VLP (RLP). In vitro assembly, disassembly and reassemb
Externí odkaz:
https://doaj.org/article/80e36f055f204d6cb3bbb5ae56e4caf0
Publikováno v:
Talanta. 80:1561-1568
A new application of intact cell MALDI-TOF MS (ICM-MS) methodology is described for monitoring the production of viral proteins and viral like particles using the baculovirus/insect cells expression system. Various MALDI matrices, cell preparation me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4319f50b64743ef705e89e691cfe0acc
https://doi.org/10.1016/j.talanta.2009.06.081
https://doi.org/10.1016/j.talanta.2009.06.081
Autor:
Paula M. Alves, Cristina Peixoto, Maria Candida M. Mellado, Pedro E. Cruz, Manuel J.T. Carrondo
Publikováno v:
Journal of Chromatography B. 874:89-94
Rotavirus VP7 is a glycoprotein that forms the viral capsid outerlayer and is essential to the correct assembly of triple-layered rotavirus-like particles (RLPs). In this work, a novel purification strategy was designed to allow obtaining highly pure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d4af35410df6719a5e9fb430b208b78
https://doi.org/10.1016/j.jchromb.2008.09.002
https://doi.org/10.1016/j.jchromb.2008.09.002
Publikováno v:
Journal of Chemical Technology & Biotechnology. 82:636-645
In this work affinity membrane adsorbers were investigated for the chromatographic purification of recombinant human erythropoietin (rhEPO) produced in mammalian cells. Cibacron Blue (CB), IDA-Cu+2, wheat germ agglutinin (WGA), concanavalin A (ConA)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0ed28d69a22e1d6c915a6c7bdebfddc3
https://doi.org/10.1002/jctb.1720
https://doi.org/10.1002/jctb.1720
Autor:
João C. Lima, Paula M. Alves, António Roldão, Maria Candida M. Mellado, Manuel J.T. Carrondo, Rui Oliveira
Publikováno v:
PLoS Computational Biology
PLoS Computational Biology, Vol 8, Iss 2, p e1002367 (2012)
PLoS Computational Biology, Vol 8, Iss 2, p e1002367 (2012)
Previous studies have reported the production of malformed virus-like-particles (VLP) in recombinant host systems. Here we computationally investigate the case of a large triple-layered rotavirus VLP (RLP). In vitro assembly, disassembly and reassemb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43feb444af59b5b51a1e453f2e01d647
http://europepmc.org/articles/PMC3280969
http://europepmc.org/articles/PMC3280969
Publikováno v:
Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009 ISBN: 9789400708839
In vitro disassembly and reassembly of virus-like particles (VLPs) can be a powerful tool for optimization of particle quality and product homogeneity. In this work single-, double- and triple-layered rotavirus-like particles (SLP, DLP and TLP, respe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b713e3e8daeeb057d1834507a1befaf5
https://doi.org/10.1007/978-94-007-0884-6_104
https://doi.org/10.1007/978-94-007-0884-6_104
Autor:
Maria Candida M. Mellado, Manuel J.T. Carrondo, António Roldão, Paula M. Alves, Leda R. Castilho
Publikováno v:
Expert review of vaccines. 9(10)
Virus-like particles (VLPs) are multiprotein structures that mimic the organization and conformation of authentic native viruses but lack the viral genome, potentially yielding safer and cheaper vaccine candidates. A handful of prophylactic VLP-based
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0806227f2a5ce30a012722f1279b3445
https://pubmed.ncbi.nlm.nih.gov/20923267
https://pubmed.ncbi.nlm.nih.gov/20923267
Autor:
Manuel J.T. Carrondo, Maria Candida M. Mellado, Paula M. Alves, Jimmy A. Mena, Laura A. Palomares, Octavio T. Ramírez
Publikováno v:
Cells and Culture ISBN: 9789048134182
Virus-like particles (VLPs) are of interest in vaccination, gene therapy and drug delivery. Their assembly process needs to be understood in order to improve their yield and quality. Triple-layered Rotavirus-like particle (TLP 2/6/7), a candidate vac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::17a52d6e45ce5ac9394ab82f72ddc0e2
https://doi.org/10.1007/978-90-481-3419-9_55
https://doi.org/10.1007/978-90-481-3419-9_55
Autor:
Paula M. Alves, Ana Varela Coelho, Catarina Franco, Maria Candida M. Mellado, Ana Luísa Simplício
Publikováno v:
Journal of chromatography. A. 1192(1)
This work describes the application of a sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) method for the analysis of triple 2/6/7 and double-layered 2/6 rotavirus-like particles (RLPs), candidate vaccines against rotavirus infection. SD
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af9254fd8e9af11171b3478e8c2e0816
https://pubmed.ncbi.nlm.nih.gov/18378247
https://pubmed.ncbi.nlm.nih.gov/18378247