Zobrazeno 1 - 10
of 112
pro vyhledávání: '"Maria Alvarado"'
Publikováno v:
MethodsX, Vol 13, Iss , Pp 102860- (2024)
In conventional cell lysate protocols, cell debris is typically discarded to obtain a cleaner lysate. However, this approach has limitations, as it may overlook vital cellular components. By discarding cell debris, researchers may inadvertently exclu
Externí odkaz:
https://doaj.org/article/2d2183ef21f0482b878ba9687d38aba3
Autor:
Matthieu Corvaisier, Jingkai Zhou, Darina Malycheva, Nicola Cornella, Dimitrios Chioureas, Nina M. S. Gustafsson, Catalina Ana Rosselló, Silvia Ayora, Tongbin Li, Kristina Ekström-Holka, Karin Jirström, Lisa Lindström, Maria Alvarado-Kristensson
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-18 (2021)
Corvaisier et al discover that γ-tubulin and replication protein PCNA forms a complex and that this facilitates recruitment of PCNA to chromatin both during cell division and during the DSB repair response. They identify a PCNA binding motif in γ-t
Externí odkaz:
https://doaj.org/article/d469a9dc7bd3404abb6d4c62729c07e8
Publikováno v:
MethodsX, Vol 8, Iss , Pp 101517- (2021)
Production of a protein of interest in bacteria and its purification from bacterial lysates are valuable tools for the purification of larger amounts of recombinant proteins. The low cost of culturing, and the rapid cell growth of bacteria make this
Externí odkaz:
https://doaj.org/article/c31348b7283140319a2f496f4e3117f2
Autor:
Maria Alvarado-Kristensson
Publikováno v:
Heliyon, Vol 6, Iss 1, Pp e03238- (2020)
More than a century ago, the centrosome was discovered and described as “the true division organ of the cell”. Electron microscopy revealed that a centrosome is an amorphous structure or pericentriolar protein matrix that surrounds a pair of well
Externí odkaz:
https://doaj.org/article/10bc3374f1ad48acbf5bbc229862d195
Autor:
Lisa Lindström, Tongbin Li, Darina Malycheva, Arun Kancharla, Helén Nilsson, Neelanjan Vishnu, Hindrik Mulder, Martin Johansson, Catalina Ana Rosselló, Maria Alvarado-Kristensson
Publikováno v:
Communications Biology, Vol 1, Iss 1, Pp 1-18 (2018)
Lisa Lindström et al. find that the gamma-tubulin cellular network is required to maintain mitochondrial function and organization in the cell. Knockdown of gamma-tubulin or loss of its GTPase domain disrupts the mitochondrial network and alters res
Externí odkaz:
https://doaj.org/article/d8b682b135e44aecbc719b783aa778cd
Autor:
Maria Alvarado-Kristensson
Publikováno v:
MethodsX, Vol 5, Iss , Pp 227-233 (2018)
When using fluorescence microscope techniques to study cells, it is essential that the cell structure and contents are preserved after preparation of the samples, and that the preparation method employed does not create artefacts that can be perceive
Externí odkaz:
https://doaj.org/article/b75100fa4aec4c03b1d2d69b4b811680
Autor:
Maria Alvarado Kristensson
Publikováno v:
Cells, Vol 10, Iss 4, p 745 (2021)
Members of the tubulin superfamily are GTPases; the activities of GTPases are necessary for life. The members of the tubulin superfamily are the constituents of the microtubules and the γ-tubulin meshwork. Mutations in members of the tubulin superfa
Externí odkaz:
https://doaj.org/article/4290337b1d1a43f28417c448bc08bcae
Autor:
Sujinna Lekmeechai, Yu-Ching Su, Marta Brant, Maria Alvarado-Kristensson, Anna Vallström, Ikenna Obi, Anna Arnqvist, Kristian Riesbeck
Publikováno v:
Frontiers in Microbiology, Vol 9 (2018)
Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory
Externí odkaz:
https://doaj.org/article/6da247d1e5684967898d1200e042fe42
Autor:
Luz H Patino, Claudia Mendez, Omaira Rodriguez, Yanira Romero, Daniel Velandia, Maria Alvarado, Julie Pérez, Maria Clara Duque, Juan David Ramírez
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 11, Iss 8, p e0005876 (2017)
In Colombia, the cutaneous leishmaniasis (CL) is the most common manifestation across the army personnel. Hence, it is mandatory to determine the species associated with the disease as well as the association with the clinical traits. A total of 273
Externí odkaz:
https://doaj.org/article/f7ae6999ffcd4c838c8dd223d3d779a7
Autor:
Catalina Ana Rosselló, Lisa Lindström, Johan Glindre, Greta Eklund, Maria Alvarado-Kristensson
Publikováno v:
Heliyon, Vol 2, Iss 9 (2016)
The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as th
Externí odkaz:
https://doaj.org/article/94c18f5494884bd19556800edac78dea